Parasitic Causes of Secretory Diarrhea in Children and Chronically Ill Adult.

Diarrhea is one of the most common reasons for people to seek medical advice - but it can range from being a mild, temporary condition, to be life threading condition. It is estimated that there are 2 billion cases of diarrheal disease every year globally, and that 1.9 million children below the age of 5 years, mostly in developing countries, die annually.

Study Overview

• Status: Completed
• Conditions: Diarrhea, Secretory
• Intervention / Treatment: Diagnostic test: stool examination; Diagnostic test: real time polymerase chain reaction

Detailed Description

Secretory diarrhea has many causes as infection with bacteria, viruses or protozoa. It results from increased chloride secretion, decreased sodium absorption, or increased mucosal permeability . Parasitic pathogens induce Secretory diarrhea as they infect and damage the absorptive villus tips, leaving Secretory crypts unbalanced, to cause net secretion and diarrhea. Parasitic causes include Cryptosporidium parvum or hominis ,Capillaria philippinensis and Giardia lamblia. Cryptosporidiosis is also recognized as a cause of prolonged and persistent diarrhea in children and persons with impaired immunity. The disease is transmitted via the fecal-oral route from infected hosts. It can be also transmitted following animal contact, ingestion of water (mainly during swimming), or through food. Cryptosporidium has emerged as the most frequently recognized cause of water-associated outbreaks of gastroenteritis. This is because of the oocyst stage in its life cycle; Cryptosporidium can resist disinfection, including chlorination, and can survive for a prolonged period in the environment.

• Study Type: Observational
• Enrollment (Actual): 100

Contacts and Locations

Study Locations

• Egypt
  • Assiut, Egypt
    • Heba gamal rashed

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: Child; Adult; Older Adult
• Accepts Healthy Volunteers: No
• Genders Eligible for Study: All

• Sampling Method: Non-Probability Sample

Study Population

-Children presented with secretory diarrhea(acute watery diarrhea) attend to out clinic lab of Assiut University pediatric hospital or admitted in pediatric hospital(gastroenterology department) during the study period.

2-adult patients having acute watery diarrhea attend to out clinic labs of Assiut University hospital or admitted in gastroenterology, nephrology and oncology department of hospital during the study period.

Description

Inclusion Criteria:

1. Children presented with secretory diarrhea(acute watery diarrhea) attend to out clinic lab of Assiut University pediatric hospital or admitted in pediatric hospital(gastroenterology department) during the study period.
2. adult patients having acute watery diarrhea attend to out clinic labs of Assiut University hospital or admitted in gastroenterology, nephrology and oncology department of hospital during the study period.

Exclusion Criteria:

1. viral and bacterial causes of diarrhea
2. Patients who were already diagnosed as intestinal inflammatory disease as irritable bowel syndrome.
3. Autoimmune conditions, such as ulcerative colitis, Crohn's disease and coeliac disease.

Study Plan

How is the study designed?

Design Details

• Observational Models: Other
• Time Perspectives: Cross-Sectional

Number of groups / cohorts

2

Cohorts and Interventions

Group / Cohort	Intervention / Treatment
study group; children with acute secretory diarrhea	Diagnostic test: stool examination; collection of stool specimen and microscopic examination for parasites; Diagnostic test: real time polymerase chain reaction; evaluation of genotype of cryptosporidium in stool specimen
Control group; normal healthy children	Diagnostic test: stool examination; collection of stool specimen and microscopic examination for parasites; Diagnostic test: real time polymerase chain reaction; evaluation of genotype of cryptosporidium in stool specimen

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
percentage of patients with positive stool analysis for cryptosporidium	Use of microscopic examination and real time polymerase chain reaction	2 days

Collaborators and Investigators

• Sponsor: Assiut University
• Investigators: Principal Investigator: Asmaa M Abd elaziz, MD, assuit university, faculty of medicine

Publications and helpful links

General Publications

• Pawlowski SW, Warren CA, Guerrant R. Diagnosis and treatment of acute or persistent diarrhea. Gastroenterology. 2009 May;136(6):1874-86. doi: 10.1053/j.gastro.2009.02.072. Epub 2009 May 7.

Study record dates

Study Major Dates

• Study Start (Actual): January 1, 2018
• Primary Completion (Actual): April 1, 2021
• Study Completion (Actual): April 12, 2021

Study Registration Dates

• First Submitted: May 24, 2017
• First Submitted That Met QC Criteria: May 24, 2017
• First Posted (Actual): May 25, 2017

Study Record Updates

• Last Update Posted (Actual): June 8, 2021
• Last Update Submitted That Met QC Criteria: June 5, 2021
• Last Verified: June 1, 2021

More Information

Terms related to this study

• Additional Relevant MeSH Terms: Pathologic Processes; Disease Attributes; Signs and Symptoms, Digestive; Diarrhea; Chronic Disease
• Other Study ID Numbers: PSDCC

Plan for Individual participant data (IPD)

• Plan to Share Individual Participant Data (IPD)?: No

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: No
• Studies a U.S. FDA-regulated device product: No