Tissue-specific Effects of Insufficient Sleep

This study plans to learn more about how sleep loss impacts the way the body responds to sugar. This work will have important implications for development of treatments and countermeasures for people who are not able to get enough sleep for various reasons.

Study Overview

• Status: Active, not recruiting
• Conditions: Healthy
• Intervention / Treatment: Behavioral: Insufficient sleep

Detailed Description

Participants will stay in the hospital for 6 days and will not be able to leave. Sleep habits will be measured for 7 days before the study. A research diet that is designed to maintain weight will be provided for 3 days before the study and during the study. Multiple tests will be performed, including metabolic testing and muscle and fat biopsies, both when participants first arrive and have had normal sleep and again after 4 nights of only 5 hours time in bed. The visit takes place at the Clinical Translational Research Center (CTRC) at the University of Colorado Denver, Anschutz Medical campus. It will take approximately 2 weeks to complete this study.

• Study Type: Interventional
• Enrollment (Actual): 18
• Phase: Not Applicable

Contacts and Locations

Study Locations

• United States
  • Colorado
    • Aurora, Colorado, United States, 80045
      • University of Colorado Anschutz Medical Campus

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 18 years to 35 years (Adult)
• Accepts Healthy Volunteers: Yes

Description

Inclusion criteria:

1. Healthy, lean participants.
2. No regular physical activity (<20 minutes/day). No exercise will be allowed for 3 days prior to study start.
3. Habitual sleep duration between 7-9.25h. Subjects must also be willing and able to keep a 9h time-in-bed schedule for 1 week prior to study admission.
4. Potential subjects must have lived at Denver altitude or higher for at least 3 months to inpatient study.

Exclusion Criteria:

1. Current or history of any clinically significant medical, psychiatric, or sleep disorder.
2. Use or history of any drugs, medications, supplements, caffeine, and alcohol.
3. Current or history of shiftwork in six months prior to laboratory study.
4. Travel more than one time zone in three weeks prior to laboratory study.
5. Blood donation in the 30 days prior to inpatient study.

Study Plan

How is the study designed?

Design Details

• Primary Purpose: Basic Science
• Allocation: N/A
• Interventional Model: Single Group Assignment
• Masking: None (Open Label)

Number of Arms

1

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
Experimental: Insufficient sleep; Each participant will receive 4 nights of insufficient sleep.	Behavioral: Insufficient sleep; Four days of insufficient sleep

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Change in insulin sensitivity	Insulin sensitivity measured by hyperinsulinemic euglycemic clamp.	One week

Secondary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Changes in macrovascular function	Macrovascular function will be assessed with flow-mediated dilation	One week
Changes in microvascular function	Macrovascular function will be assessed with EndoPAT	One week
Changes in cognitive abilities	Cognitive computer tests performed during study	One week
Changes in circadian rhythms	Salivary dim-light melatonin offset will be assessed	One week
Changes in metabolic tissue function	Tissue biopsies will be performed	One week

Collaborators and Investigators

• Sponsor: University of Colorado, Denver
• Investigators: Principal Investigator: Josiane L. Broussard, PhD, University of Colorado, Boulder

Publications and helpful links

Helpful Links

• Full list of publications

Study record dates

Study Major Dates

• Study Start (Actual): February 12, 2018
• Primary Completion (Estimated): June 30, 2024
• Study Completion (Estimated): December 30, 2024

Study Registration Dates

• First Submitted: February 6, 2018
• First Submitted That Met QC Criteria: February 12, 2018
• First Posted (Actual): February 19, 2018

Study Record Updates

• Last Update Posted (Actual): November 30, 2023
• Last Update Submitted That Met QC Criteria: November 28, 2023
• Last Verified: November 1, 2023

More Information

Terms related to this study

• Keywords: Sleep; Blood Sugar
• Additional Relevant MeSH Terms: Mental Disorders; Nervous System Diseases; Dyssomnias; Sleep Wake Disorders; Neurologic Manifestations; Sleep Deprivation
• Other Study ID Numbers: 17-0533

Plan for Individual participant data (IPD)

• Plan to Share Individual Participant Data (IPD)?: NO

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: No
• Studies a U.S. FDA-regulated device product: No