- ICH GCP
- US Clinical Trials Registry
- Clinical Trial NCT03838822
Kinetics of Metabolic Cofactors in NAFLD (NAFLDCOFCAL)
Kinetics of Metabolic Cofactors After Oral Supplementation in Healthy Subjects
There is a strong correlation between major adverse health consequences of obesity and development of non-alcoholic fatty liver disease (NAFLD). NAFLD is characterized by abnormal hepatic accumulation of triglycerides and other lipids. It has become a worldwide health problem that accelerates cirrhosis, type 2 diabetes mellitus (T2DM), and especially premature cardiovascular morbidity and mortality.
The plasma level of glutathione (GSH) is typically depleted in individuals with metabolism related disorders. However, cellular GSH levels cannot be increased by supplementing GSH and it must be synthesized within the liver either de novo or by salvation pathway. The level of GSH is not enough to maintain and regulate the thiol redox status of the liver in subjects with high hepatic steatosis at fasting stage due to the depletion of glycine. Glycine can be synthesized via the interconversion of serine. It has been shown that the serine synthesis is downregulated in patients with NAFLD and supplementation of serine has attenuated alcoholic fatty liver by enhancing homocysteine metabolism in mice and rats. Depleted liver glutathione is also restored by the administration of N-acetylcystein as in acetaminophen poising. L-carnitine and nicotinamide that both stimulate the transfer of fatty acids from cytosol to mitochondria have been identified as two additional cofactors that are depleted in patients with NAFLD.
In this study, the kinetics in blood of pivotal metabolic cofactors, serine, L-carnitine, N-acetylcystein and nicotinamide after single and simultaneous dietary supplementation, are measured.
Study Overview
Detailed Description
In 10 healthy subjects with BMI <30 kg/m2 the plasma concentrations of 4 natural compounds (nicotinamide riboside, L-carnitine, L-serine and N-acetylcystein) are measured by ultra-performance liquid chromatography-tandem mass spectrometry (UPLCMSMS) after individual and combined administration, on five consecutive days and at every hour during 9 hours after administration. The study will start at 8:00 every morning and at each time point, blood samples will be collected.
The taste and any potential sensing of the co-factors such as vertigo, nausea, bowel movement will be recorded.
Each participant will receive one oral dose of Day 1: 1 g nicotinamide riboside; Day 2: 3 g L-carnitine; Day 3: 5 g N-acetylcystein; Day 4: 20 g L-serine; Day 5: combined 1 g nicotinamide riboside, 3 g L-carnitine, 5 g N-acetylcystein, and 20 g L-serine
In addition, untargeted metabolomics analysis as well as O-link proteomics analysis we be performed to study effect of the administered cofactors.
Study Type
Enrollment (Actual)
Phase
- Early Phase 1
Contacts and Locations
Study Locations
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Göteborg, Sweden, 411 31
- Hanns-Ulrich Marschall
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Göteborg, Sweden
- Sahlgrenska Academy
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Participation Criteria
Eligibility Criteria
Ages Eligible for Study
Accepts Healthy Volunteers
Genders Eligible for Study
Description
Inclusion Criteria:
Healthy without any medication, no smokers, no obesity
Exclusion Criteria:
Any known disease, obesity
Study Plan
How is the study designed?
Design Details
- Primary Purpose: TREATMENT
- Allocation: NA
- Interventional Model: SINGLE_GROUP
- Masking: NONE
Arms and Interventions
Participant Group / Arm |
Intervention / Treatment |
---|---|
EXPERIMENTAL: Intervention
Oral administration of cofactors, 1g nicotinamide riboside, 3g L-carnitine, 20g serine and 5g N-acetylcystein, first as single compounds, then combined, on 5 days
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Oral administration of 1g nicotinamide riboside, 3g L-carnitine, 20g serine and 5g N-acetylcystein, first as single compounds, then combined, on 5 days
|
What is the study measuring?
Primary Outcome Measures
Outcome Measure |
Measure Description |
Time Frame |
---|---|---|
Changes of plasma levels of nicotinamide riboside measured by mass spectrometry
Time Frame: Twenty-four hours after administration
|
Plasma levels of nicotinamide riboside by UPLCMSMS at 8 time points during 24h after administration
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Twenty-four hours after administration
|
Changes of plasma levels of L-carnitine measured by mass spectrometry
Time Frame: Twenty-four hours after administration
|
Plasma levels of L-carnitine (nM) measured by UPLCMSMS at 8 time points during 24h after administration
|
Twenty-four hours after administration
|
Changes of plasma levels of L-serine measured by mass spectrometry
Time Frame: Twenty-four hours after administration
|
Plasma levels of L-serine (nM) measured by UPLCMSMS at 8 times points during 24h after administration
|
Twenty-four hours after administration
|
Changes of plasma levels of N-acetylcystein measured by mass spectrometry
Time Frame: Twenty-four hours after administration
|
Plasma levels of N-acetylcystein (nM) measured by UPLCMSMS at 8 times points during 24h after administration
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Twenty-four hours after administration
|
Secondary Outcome Measures
Outcome Measure |
Measure Description |
Time Frame |
---|---|---|
Changes in the plasma level of metabolites associated with the supplementation of metabolic co-factors.
Time Frame: Twenty-four hours after administration of combined cofactors
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Untargeted metabolomic analysis using mass spectrometry.
(Untargeted = not pre-specified in terms of outcome)
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Twenty-four hours after administration of combined cofactors
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Changes in the plasma level of inflammation-related proteins associated with the supplementation of metabolic co-factors.
Time Frame: Twenty-four hours after administration of combined cofactors
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Changes in the plasma level of inflammation related proteins associated with the supplementation of metabolic co-factors.
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Twenty-four hours after administration of combined cofactors
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Collaborators and Investigators
Investigators
- Principal Investigator: Hanns-Ulrich Marschall, Prof, Sahlgrenska University Hospital, Sweden
Study record dates
Study Major Dates
Study Start (ACTUAL)
Primary Completion (ACTUAL)
Study Completion (ACTUAL)
Study Registration Dates
First Submitted
First Submitted That Met QC Criteria
First Posted (ACTUAL)
Study Record Updates
Last Update Posted (ACTUAL)
Last Update Submitted That Met QC Criteria
Last Verified
More Information
Terms related to this study
Other Study ID Numbers
- Cofactor Calibration 1.0
Plan for Individual participant data (IPD)
Plan to Share Individual Participant Data (IPD)?
Drug and device information, study documents
Studies a U.S. FDA-regulated drug product
Studies a U.S. FDA-regulated device product
This information was retrieved directly from the website clinicaltrials.gov without any changes. If you have any requests to change, remove or update your study details, please contact register@clinicaltrials.gov. As soon as a change is implemented on clinicaltrials.gov, this will be updated automatically on our website as well.
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