Can Amino Acid Supplementation Augment the Anabolic Response in Tendon After Exercise

The goal of this study is to determine if an oral provision of amino acids will enhance exercise-induced increases in markers of Achilles tendon collagen production.

Study Overview

• Status: Completed
• Conditions: Tendinopathy
• Intervention / Treatment: Dietary supplement: Amino Acids

Detailed Description

Tendon pain is a common complaint in older adults. The mechanisms contributing to the large number of tendon problems in older adults are not known. It has been shown that tendon cross-sectional area (CSA) is reduced with aging concurrent with a significant loss of tendon collagen. Exercise is used to reverse the loss of muscle mass with aging but surprisingly exercise interventions designed to reduce tendon problems in older people are relatively unexplored. In young adults, exercise stimulates tendon collagen synthesis and enhances the production of growth factors, which stimulate tendon collagen production. It is presumed that exercise-induced increase in collagen synthesis contributes to the increase in tendon stiffness and CSA noted in young adults after chronic resistance exercise (RE). In contrast, our preliminary work indicates that RE is not effective at reducing the effects of aging on tendon. The investigators have demonstrated that 12-weeks of RE does not lead to increases in tendon CSA or stiffness, even when skeletal muscle mass and strength are greatly enhanced. To prevent tendon injury, greater tendon CSA and stiffness aid in withstanding the force generated by trained skeletal muscle. Greater CSA and stiffness also optimize force transfer through tendon to maximize musculoskeletal function. Our findings indicate that RE is not an optimal intervention for reversing age-related changes in tendon properties. Novel interventions are clearly needed that will enhance collagen synthesis leading to an increase in tendon stiffness and CSA while also improving skeletal muscle mass and strength in older individuals. This study will evaluate the effectiveness of various methods for stimulating tendon collagen synthesis during a bout of acute exercise. Specifically, the investigators propose two approaches to this problem.

1. An acute bout of cycling exercise will enhance the release of growth factors leading to a larger increase in tendon collagen synthesis.
2. A bolus of amino acids given orally will increase the release of key stimulators of tendon collagen synthesis leading to enhanced collagen synthesis after acute exercise.

• Study Type: Interventional
• Enrollment (Actual): 7
• Phase: Not Applicable

Contacts and Locations

Study Locations

• United States
  • Indiana
    • West Lafayette, Indiana, United States, 47907
      • Purdue University

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 21 years to 80 years (Adult, Older Adult)
• Accepts Healthy Volunteers: Yes

Description

Inclusion Criteria:

• 21-35 years or 60-80 years old

Exclusion Criteria:

• diabetes
• BMI>35
• acute illness, cardiac, pulmonary, liver, or kidney abnormalities, uncontrolled hypertension, metabolic disorders, arthritis, or a history of neuromuscular problems.
• chronic pain medication users
• alcoholism
• competitive athletes
• pregnant women will be excluded
• Individuals with specific allergies to amino acids

Study Plan

How is the study designed?

Design Details

• Primary Purpose: Basic Science
• Allocation: Randomized
• Interventional Model: Crossover Assignment
• Masking: Single

Number of Arms

2

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
Experimental: Young; 21-30 year old men and women Inclusion/Exclusion Criteria; All subjects will be sedentary or recreationally active (sedentary: one day or less per week of aerobic or resistance exercise for at least a year or recreationally active: not training for competitive events).; Age: 21-30 years; Body mass index <35 kg•m-2,; Chronic users of medications such as acetaminophen, ibuprofen, or prescription cyclooxygenase inhibitors will be excluded as these medications have been shown to alter tendon physiology.; Individuals with diabetes will be excluded. Diabetes is thought to change the structure of tendon.; Individuals with a history of tendon pain will be excluded, as chronic tendon pain is associated with changes in the structure of tendon.; Persons with allergies/sentivitiy to amino acids or ingedianet of Cystal Light will be excluded.	Dietary supplement: Amino Acids; Oral bolus of essential amino acids
Experimental: Older Adults; Inclusion/Exclusion Criteria; All subjects will be sedentary or recreationally active (sedentary: one day or less per week of aerobic or resistance exercise for at least a year or recreationally active: not training for competitive events).; Age: 60-75 years; Body mass index <35 kg•m-2; Chronic users of medications such as acetaminophen, ibuprofen, or prescription cyclooxygenase inhibitors will be excluded as these medications have been shown to alter tendon physiology.; Individuals with diabetes will be excluded. Diabetes is thought to change the structure of tendon.; Individuals with a history of tendon pain will be excluded, as chronic tendon pain is associated with changes in the structure of tendon.; Persons with allergies/sentivitiy to amino acids or ingedianet of Cystal Light will be excluded.	Dietary supplement: Amino Acids; Oral bolus of essential amino acids

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Achilles peritendinous concentrations of pro-collagen	Peritendinous samples will be collected using microdialysis. Pro-collagen will be assessed using enzyme immunoassay kits	0-6 hours post consumption
Amino Acid Levels	Achilles peritendinous amino acid concentrations will be assess using high performance liquid chromatography.	0-6 hours post consumption

Collaborators and Investigators

• Sponsor: Purdue University

Study record dates

Study Major Dates

• Study Start (Actual): May 9, 2017
• Primary Completion (Actual): December 13, 2022
• Study Completion (Actual): December 20, 2022

Study Registration Dates

• First Submitted: August 20, 2019
• First Submitted That Met QC Criteria: August 22, 2019
• First Posted (Actual): August 26, 2019

Study Record Updates

• Last Update Posted (Actual): April 11, 2023
• Last Update Submitted That Met QC Criteria: April 9, 2023
• Last Verified: April 1, 2023

More Information

Terms related to this study

• Keywords: exercise; tendon; amino acid
• Additional Relevant MeSH Terms: Wounds and Injuries; Musculoskeletal Diseases; Muscular Diseases; Tendon Injuries; Tendinopathy
• Other Study ID Numbers: 1704019133

Plan for Individual participant data (IPD)

• Plan to Share Individual Participant Data (IPD)?: NO

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: No
• Studies a U.S. FDA-regulated device product: No
• product manufactured in and exported from the U.S.: No