Effects of a Single Bout of Resistance Training Session on Hormonal Response in Pre-pubertal and Pubertal Boys

: Resistance training induces strength gains in both children and adolescents. Exercise training is linked to anabolic functions through the GH-IGF-I axis. This has been demonstrated in studies showing a higher activity of GH and IGF-I in fitter adolescents and adults. Exercise also stimulates pro-inflammatory cytokines (IL-1β, IL-6, TNF-α) that suppress the GH-IGF-I axis. This has been observed in endurance-type training, while resistance training has received less attention.

Boys experience a significant physiological muscle-growth change during puberty that is associated with increases in boys androgen levels. How maturation affects the hormonal response to resistance training in boys is unclear. Therefore, the purpose of the study is to compare the acute effects of a single bout of resistance training on hormones and pro-inflammatory cytokines in pre-pubertal and pubertal boys.

Study Overview

• Status: Not yet recruiting
• Conditions: Cytokines; Hormones
• Intervention / Treatment: Other: Resistance Training

Detailed Description

Preliminary measurements:

Preliminary testing will include anthropometric measurements of body mass, height, body fat percentage, and lean leg volume (LLV). The maturation stage will be determined according to the Tanner scale. There will also be an assessment of maximal strength testing to determine exercise intensity for each individual. Two familiarization sessions will be conducted for muscular strength before the muscular strength testing session.

Main trial A venous blood sample will be collected pre-exercise at resting condition. The trial will start with a standardized warm-up followed by a short (20-30 min), high-intensity whole-body resistance training session. Venous blood samples will be collected immediately after the completion of the training session and during the recovery period.

• Study Type: Interventional
• Enrollment (Anticipated): 40
• Phase: Not Applicable

Contacts and Locations

• Study Contact: Name: Apostolos Theos, PhD; Phone Number: +46702811565; Email: apostolos.theos@umu.se

Study Locations

• Sweden
  • Västerbotten
    • Umeå, Västerbotten, Sweden, 901 87
      • Umeå Movement and Exercise Laboratory, Umeå University
      • Contact: Apostolos Theos, PhD; Phone Number: +46702811565; Email: apostolos.theos@umu.se
      • Contact: Tobias Stenlund, PhD; Phone Number: +46907868040; Email: tobias.stenlund@umu.se

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 9 years to 17 years (Child)
• Accepts Healthy Volunteers: Yes
• Genders Eligible for Study: Male

Description

Inclusion Criteria:

• Healthy boys (n=40)
• Physically active, defined as a minimum of 2 years experience in a sports club
• Biological age range: 20 pre-pubertal (Tanner I-II) - 20 pubertal (Tanner III-IV) boys
• No previous experience of structured resistance training

Exclusion Criteria:

• Chronic diseases that affect full participation
• Injuries that limit full participation

Study Plan

How is the study designed?

Design Details

• Primary Purpose: Other
• Allocation: Non-Randomized
• Interventional Model: Parallel Assignment
• Masking: Single

Number of Arms

2

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
Experimental: Resistance Training Session; Resistance training. Heavy resistance training. 10 reps x 3 set of upper- and lower-body resistance exercises	Other: Resistance Training; Both groups will perform a resistance training session consisted of 10 reps x 3 set of upper- and lower-body resistance exercises.
No Intervention: Control; Control activity. None-exercising. 30 min of rest

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Growth Hormone Baseline	Blood Concentration of Growth Hormone	Baseline testing before the exercise intervention (Pre values)
Change from Baseline Growth Hormone Post exercise	Blood Concentration of Growth Hormone	Right after the completion of the exercise intervention (Post values)
Change from Baseline Growth Hormone 15 minutes post exercise	Blood Concentration of Growth Hormone	15 minutes after the completion of the exercise intervention (Post-15 values)
Change from Baseline Growth Hormone 30 minutes post exercise	Blood Concentration of Growth Hormone	30 minutes after the completion of the exercise intervention (Post-30 values)
Insulin-like Growth Factor-1 Baseline	Blood concentration of Insulin-like Growth Factor-1	Baseline testing before the exercise intervention (Pre values)
Change from Baseline Insulin-like Growth Factor-1 Post exercise	Blood concentration of Insulin-like Growth Factor-1	Right after the completion of the exercise intervention (Post values)
Change from Baseline Insulin-like Growth Factor-1 15 minutes Post exercise	Blood concentration of Insulin-like Growth Factor-1	15 minutes after the completion of the exercise intervention (Post-15 values)
Change from Baseline Insulin-like Growth Factor-1 30 minutes Post exercise	Blood concentration of Insulin-like Growth Factor-1	30 minutes after the completion of the exercise intervention (Post-30 values)

Secondary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Cortisol Baseline	Blood concentration of Cortisol	Baseline testing before the exercise intervention (Pre values)
Change from Baseline Cortisol Post exercise	Blood concentration of Cortisol	Right after the completion of the exercise intervention (Post values)
Change from Baseline Cortisol 15 minutes Post exercise	Blood concentration of Cortisol	15 minutes after the completion of the exercise intervention (Post-15 values)
Change from Baseline Cortisol 30 minutes Post exercise	Blood concentration of Cortisol	30 minutes after the completion of the exercise intervention (Post-30 values)
Testosterone Baseline	Blood concentration of testosterone	Baseline testing before the exercise intervention (Pre values)
Change from Baseline Testosterone Post Exercise	Blood concentration of testosterone	Right after the completion of the exercise intervention (Post values)
Change from Baseline Testosterone 15 minutes Post Exercise	Blood concentration of testosterone	15 minutes after the completion of the exercise intervention (Post-15 values)
Change from Baseline Testosterone 30 minutes Post Exercise	Blood concentration of testosterone	30 minutes after the completion of the exercise intervention (Post-30 values)
Interleukin-6 Baseline	Blood concentration of Interleukin-6	Baseline testing before the exercise intervention (Pre values)
Change from Baseline Interleukin-6 Post Exercise	Blood concentration of Interleukin-6	Right after the completion of the exercise intervention (Post values)
Change from Baseline Interleukin-6 15 minutes Post Exercise	Blood concentration of Interleukin-6	15 minutes after the completion of the exercise intervention (Post-15 values)
Change from Baseline Interleukin-6 30 minutes Post Exercise	Blood concentration of Interleukin-6	30 minutes after the completion of the exercise intervention (Post-30 values)
Interleukin-1 alpha Baseline	Blood concentration of Interleukin-1 alpha	Baseline testing before the exercise intervention (Pre values)
Change from Baseline Interleukin-1 alpha Post Exercise	Blood concentration of Interleukin-1 alpha	Right after the completion of the exercise intervention (Post values)
Change from Baseline Interleukin-1 alpha 15 minutes Post Exercise	Blood concentration of Interleukin-1 alpha	15 minutes after the completion of the exercise intervention (Post-15 values)
Change from Baseline Interleukin-1 alpha 30 minutes Post Exercise	Blood concentration of Interleukin-1 alpha	30 minutes after the completion of the exercise intervention (Post-30 values)
Tumor Necrosis Factor alpha Baseline	Blood concentration of Tumor Necrosis Factor alpha	Baseline testing before the exercise intervention (Pre values)
Change from Baseline Tumor Necrosis Factor alpha Post Exercise	Blood concentration of Tumor Necrosis Factor alpha	Right after the completion of the exercise intervention (Post values)
Change from Baseline Tumor Necrosis Factor alpha 15 minutes Post Exercise	Blood concentration of Tumor Necrosis Factor alpha	15 minutes after the completion of the exercise intervention (Post-15 values)
Change from Baseline Tumor Necrosis Factor alpha 30 minutes Post Exercise	Blood concentration of Tumor Necrosis Factor alpha	30 minutes after the completion of the exercise intervention (Post-30 values)
Sex Hormone Binding Globulin Baseline	Blood concentration of Sex Hormone Binding Globulin	Baseline testing before the exercise intervention (Pre values)
Change from Baseline Baseline Sex Hormone Binding Post Exercise	Blood concentration of Sex Hormone Binding Globulin	Right after the completion of the exercise intervention (Post values)
Change from Baseline Baseline Sex Hormone Binding 15 minutes Post Exercise	Blood concentration of Sex Hormone Binding Globulin	15 minutes after the completion of the exercise intervention (Post-15 values)
Change from Baseline Sex Hormone Binding Globulin 30 minutes Post Exercise	Blood concentration of Sex Hormone Binding Globulin	30 minutes after the completion of the exercise intervention (Post-30 values)

Collaborators and Investigators

• Sponsor: Umeå University
• Investigators: Principal Investigator: Apostolos Theos, PhD, Umeå University

Study record dates

Study Major Dates

• Study Start (Anticipated): September 1, 2021
• Primary Completion (Anticipated): December 1, 2021
• Study Completion (Anticipated): March 1, 2022

Study Registration Dates

• First Submitted: June 11, 2021
• First Submitted That Met QC Criteria: August 20, 2021
• First Posted (Actual): August 26, 2021

Study Record Updates

• Last Update Posted (Actual): August 26, 2021
• Last Update Submitted That Met QC Criteria: August 20, 2021
• Last Verified: August 1, 2021

More Information

Terms related to this study

• Other Study ID Numbers: 2020-03179

Plan for Individual participant data (IPD)

• Plan to Share Individual Participant Data (IPD)?: NO

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: No
• Studies a U.S. FDA-regulated device product: No