The Age-related Changes of Energy Metabolism in Physically Fit Adult Males

The Influence of Age and Physical Fitness Level on the Level of Energy Metabolism and Antioxidant Capacity in Adult Males

The influence of age and physical fitness level on the level of energy metabolism and antioxidant capacity in adult males.

Study Overview

• Status: Completed
• Conditions: Healthy
• Intervention / Treatment: Diagnostic test: Cardio-pulmonary exercise test

Detailed Description

Over four hundred healthy, adult males of different age categories and physical fitness levels were included in the study. There is much research convincing that in athletes of various sports disciplines the level of energy metabolism, oxidative stress, and amino acid profiles during years of training undergoes sports adaptation. The study aimed to evaluate how age and physical fitness level affects the level of purine metabolites and metabolites of nicotinamide adenine dinucleotide, the level of antioxidant capacity, and amino acid profiles. Investigators hypothesized that there will be an age-related decline in most of the physiological indices, but significant differences will appear regarding the level of an individual's sports adaptation.

• Study Type: Interventional
• Enrollment (Actual): 420
• Phase: Not Applicable

Contacts and Locations

Study Locations

• Poland
  • Poznań, Poland, 61-871
    • Poznań University of Physical Education

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 18 years and older (ADULT, OLDER_ADULT)
• Accepts Healthy Volunteers: Yes
• Genders Eligible for Study: Male

Description

Inclusion Criteria:

1. healthy
2. non-smoking
3. physically fit, including top-level athletes

Exclusion Criteria:

1. no reported history of a cardiopulmonary or renal diseases, and other chronic diseases
2. no major orthopedic injury or illness resulting in an inability to run
3. no medications that could affect cardiopulmonary functions
4. normal resting electrocardiogram
5. body mass index (BMI) below 30.0 kg·m-2
6. normal erythrocyte count and Hb content
7. no pathological states known of significantly elevated adenylate pool and concentration of ATP, e.g. sickle cell disease, diabetes, leukemia, sepsis, tuberculosis, meningococcal infection, or renal insufficiency

Study Plan

How is the study designed?

Design Details

• Primary Purpose: BASIC_SCIENCE
• Allocation: NA
• Interventional Model: SINGLE_GROUP
• Masking: NONE

Number of Arms

1

Arms and Interventions

Participant Group / Arm

Intervention / Treatment

EXPERIMENTAL: Healthy, physically fit men; All participants underwent a single CPET test with pre- and post-exercise blood measurements.

Diagnostic test: Cardio-pulmonary exercise test; An incremental running test was conducted on the treadmill. Initially, the exercise protocol included standing still for 3 min and walking for 3 min at a constant speed of 4 km/h. After this introductory part, the speed was increased to 8 km/h and then every 3 min by 2 km/h until the volitional exhaustion was reached. During all procedure oxygen consumption was measured with the use of a portable breath-by-breath ergospirometer.

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Cardio-respiratory measures	Cardiopulmonary exercise test (CPET) was conducted while an incremental running test on the treadmill using a portable breath-by-breath ergospirometer and heart rate monitor.	during the intervention

Secondary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Blood Lactate level	Lactate concentration was performed by the spectrophotometric enzymatic method.	baseline and immediately after the intervention
Plasma purine nucleotides	The plasma purine metabolites were estimated in plasma using high-performance liquid chromatography (HPLC) with UV-VIS detection. Measurements performed: Guanosine, Inosine, Adenosine, Hypoxanthine, Xanthine, Uric Acid, Allantoin, Adenine Nucleotides [adenosine triphosphate (ATP), adenosine diphosphate (ADP), adenosine monophosphate (AMP), inosine monophasphate (IMP)], Guanine Nucleotides [guanosine triphosphate (GTP), guanosine diphosphate (GDP), guanosine monophosphate (GMP)].	baseline and immediately after the intervention
Erythrocyte Purine Metabolites	The erythrocyte purine metabolites were estimated in erythrocytes using high-performance liquid chromatography (HPLC) with UV-VIS detection. Measurements performed: Adenine Nucleotides [adenosine triphosphate (ATP), adenosine diphosphate (ADP), adenosine monophosphate (AMP), inosine monophasphate (IMP)], Guanine Nucleotides [guanosine triphosphate (GTP), guanosine diphosphate (GDP), guanosine monophosphate (GMP)].	baseline and immediately after the intervention
Hypoxanthine-guanine phosphoribosyltransferase (HGPRT) activity	The HGPRT activity was estimated in whole blood and in erythrocytes using high-performance liquid chromatography (HPLC) with UV-VIS detection.	baseline and immediately after the intervention
Nicotinamide Adenine Dinucleotide (NAD) and its metabolites	Nicotinamide Adenine Dinucleotide (NAD) and its metabolites were estimated in plasma using mass spectrometric procedure. Measurements performed: Nicotinamide Adenine Dinucleotide (NAD+), Nicotinamide (NA), 1-Methylnicotinamide (MNA), 1-methyl-2-pyridone-5-carboxamide (M2PY), 1-methyl-4-pyridone-5-carboxamide (M4PY), Nicotinamide Mononucleotide (NMN), 4-pyridone-3-carboxamide ribonucleoside (4PYR)	baseline and immediately after the intervention
Total antioxidant status (TAS)	Total antioxidant status (TAS) measurement was performed using commercially available ELISA immunoassay.	baseline
Thiobarbituric-acid-reactive substances (TBARS)	The measurement of thiobarbituric-acid-reactive substances (TBARS) was performed using commercially available ELISA immunoassay.	baseline
Malondialdehyde (MDA)	The measurement of malondialdehyde (MDA) was performed using commercially available ELISA immunoassay.	baseline
Polyphenols	The measurement of polyphenols was performed using commercially available ELISA immunoassay.	baseline
Reduced glutathione (GSH)	The measurement of reduced glutathione (GSH) was performed using commercially available ELISA immunoassay.	baseline
Glutathione peroxidase (GPx) activity	The measurement of glutathione peroxidase (GPx) activity was performed using commercially available ELISA immunoassay.	baseline
Aminoacid profile	The levels of 20 aminoacids were estimated in plasma using mass spectrometric procedure.	baseline

Collaborators and Investigators

• Sponsor: Poznan University of Physical Education
• Investigators: Principal Investigator: Jacek Zieliński, Prof., Poznań University of Physical Education

Study record dates

Study Major Dates

• Study Start (ACTUAL): January 1, 2011
• Primary Completion (ACTUAL): November 30, 2019
• Study Completion (ACTUAL): December 20, 2019

Study Registration Dates

• First Submitted: October 7, 2021
• First Submitted That Met QC Criteria: October 29, 2021
• First Posted (ACTUAL): November 9, 2021

Study Record Updates

• Last Update Posted (ACTUAL): November 16, 2021
• Last Update Submitted That Met QC Criteria: November 10, 2021
• Last Verified: September 1, 2021

More Information

Terms related to this study

• Keywords: athletes; CPET; masters; purine metabolites; NAD metabolites
• Other Study ID Numbers: AWF_LA_MA865862

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: No
• Studies a U.S. FDA-regulated device product: No