The Effect Of Anaerobic Exercise On Bone Morphogenetic Protein 9

Bone morphogenetic protein (BMP) 9 from the BMP family has an important role in the remodeling of bone and cartilage. Due to these features, BMP9 can play an important role in especially anaerobic exercise training adaptations. However, the role of anaerobic exercise in these effects is still unclear. The aim of this study is to investigate the role of BMP9 on the effects of acute and chronic anaerobic exercise on bone metabolism markers. Postprandial venous blood samples were taken pre- and post- acute YOYO intermittent recovery test (level 1) in 40 trained male athletes aged 18-35 who did anaerobic sports (such as basketball, volleyball and handball) and 41 sedentary volunteers aged 18-35. Serum BMP9, as markers of bone formation and resorption; alkaline phosphatase (ALP), C-terminal telopeptide of type 1 collagen (CTX1), cartilage oligomeric matrix protein (COMP) and calcium levels were determined. The data to be obtained as a result of our study can give information about the relationship between the skeletal and bone health of the athletes and anaerobic exercise.

Study Overview

• Status: Active, not recruiting
• Conditions: Healthy; Exercise
• Intervention / Treatment: Other: Physiological test; Other: Physical test; Other: Biochemical tests

• Study Type: Observational
• Enrollment (Actual): 81

Contacts and Locations

Study Locations

• Turkey
  • İzmir, Turkey, 35040
    • Ege University Faculty of Sport Sciences

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 18 years to 35 years (Adult)
• Accepts Healthy Volunteers: Yes
• Genders Eligible for Study: Male

• Sampling Method: Probability Sample

Study Population

Athletic group: trained male athletes who did anaerobic sports (such as basketball, volleyball and handball) Control group: sedentary males

Description

Inclusion Criteria:

• being male
• having a body mass index of less than 30 being

Exclusion Criteria:

• to have some disease or disability
• to regularly use cigarettes and alcohol
• to use any medication or antioxidant substance

Study Plan

How is the study designed?

Number of groups / cohorts

2

Cohorts and Interventions

Group / Cohort	Intervention / Treatment
Athletic group; Healthy male athletes who have been doing regular anaerobic sports (such as basketball, volleyball and handball) for at least 3 months	Other: Physiological test; This test was conducted to determine the endurance levels of the participants.; Other Names: YOYO intermittent recovery test (level 1); Other: Physical test; Height, weight, and BMI were determined; Other: Biochemical tests; BMP9, CTX1, COMP, oxLDL protein levels, ALP, GGT, TAS, TOS, OSI, NO, calcium, T-C, LDL-C, Triglyceride, HDL-C, glucose, CRP, AST, ALT, erythrocyte, mean erythrocyte volume (MCV), hematocrit, leukocyte, thrombocyte levels were determined from blood samples.
Control group; Healthy sedentary (not doing regular sports for at least 3 months) men with age and physical characteristics similar to the athlete group	Other: Physiological test; This test was conducted to determine the endurance levels of the participants.; Other Names: YOYO intermittent recovery test (level 1); Other: Physical test; Height, weight, and BMI were determined; Other: Biochemical tests; BMP9, CTX1, COMP, oxLDL protein levels, ALP, GGT, TAS, TOS, OSI, NO, calcium, T-C, LDL-C, Triglyceride, HDL-C, glucose, CRP, AST, ALT, erythrocyte, mean erythrocyte volume (MCV), hematocrit, leukocyte, thrombocyte levels were determined from blood samples.

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Change from serum BMP9 levels at pre- and post- YOYO intermittent test	Postprandial venous blood samples were obtained before and after acute YOYO IR test (level 1). The YOYO test lasted 10-20 minutes depending on the ability level of the participants. A blood sample was taken 15 minutes before and 15 minutes after the YOYO test. From these blood samples, serum Bone morphogenetic protein 9 (BMP9) was determined.	between 40-50 minutes
Change from serum CTX1 levels at pre- and post- YOYO intermittent test	Postprandial venous blood samples were obtained before and after acute YOYO IR test (level 1). The YOYO test lasted 10-20 minutes depending on the ability level of the participants. A blood sample was taken 15 minutes before and 15 minutes after the YOYO test. From these blood samples, serum C-terminal telopeptide of Type 1 collagen (CTX1) was determined.	between 40-50 minutes
Change from serum COMP levels at pre- and post- YOYO intermittent test	Postprandial venous blood samples were obtained before and after acute YOYO IR test (level 1). The YOYO test lasted 10-20 minutes depending on the ability level of the participants. A blood sample was taken 15 minutes before and 15 minutes after the YOYO test. From these blood samples, serum cartilage oligomeric matrix protein (COMP) was determined.	between 40-50 minutes

Collaborators and Investigators

• Sponsor: Ege University
• Investigators: Study Director: Faruk Turgay, Ege University Faculty of Sport Sciences

Study record dates

Study Major Dates

• Study Start (Actual): December 3, 2020
• Primary Completion (Actual): June 1, 2021
• Study Completion (Anticipated): December 1, 2021

Study Registration Dates

• First Submitted: October 11, 2021
• First Submitted That Met QC Criteria: November 2, 2021
• First Posted (Actual): November 11, 2021

Study Record Updates

• Last Update Posted (Actual): November 11, 2021
• Last Update Submitted That Met QC Criteria: November 2, 2021
• Last Verified: November 1, 2021

More Information

Terms related to this study

• Keywords: Bone morphogenetic protein; BMP9; anaerobic exercise
• Other Study ID Numbers: TDK-2020-22186 (Other Grant/Funding Number: Ege University Scientific Research Project Coordination Unit)

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: No
• Studies a U.S. FDA-regulated device product: No