Influence of Endurance Exercise and Histamine Receptors on the Gene Expression in Skeletal Muscle

Influence of Endurance Training and Histamine Receptor Antagonists on the Transcriptome Response in Human Muscle

Blocking histamine H1/H2 receptors blunts chronic endurance training adaptations. The current study addresses a twofold research question: "What is the influence of endurance training (1) and histamine H1 and H2 signaling (2) on the gene expression in human skeletal muscle." Results from this study will yield more insights into the molecular mechanisms of adaptations to exercise training.

Study Overview

• Status: Completed
• Conditions: Exercise; Histamine
• Intervention / Treatment: Drug: Placebo: Placebo + exercise training.; Drug: H1 blockade: H1 receptor antagonist + exercise training; Drug: H2 Blockade: H2 receptor antagonist + exercise training

• Study Type: Interventional
• Enrollment (Actual): 14
• Phase: Not Applicable

Contacts and Locations

Study Locations

• Belgium
  • Oost-Vlaanderen
    • Ghent, Oost-Vlaanderen, Belgium, 9000
      • Department of movement and sports sciences, Ghent University, Belgium

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 18 years to 45 years (Adult)
• Accepts Healthy Volunteers: Yes

Description

Inclusion Criteria:

• Male and female
• 18-45 years
• not to medium physically active

Exclusion Criteria:

• Smoking
• Chronic disease
• Supplement or medication intake
• Seasonal allergies

Study Plan

How is the study designed?

Design Details

• Primary Purpose: Basic Science
• Allocation: Randomized
• Interventional Model: Crossover Assignment
• Masking: Triple

Number of Arms

3

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
Placebo Comparator: 1: Placebo; lactose	Drug: Placebo: Placebo + exercise training.; Oral placebo Exercise training: Interval-based cycling exercise
Experimental: H1 blockade; 540 mg Fexofenadine	Drug: H1 blockade: H1 receptor antagonist + exercise training; H1 blockade: oral blockade Exercise training: Interval-based cycling exercise
Experimental: H2 blockade; 40 mg Famotidine	Drug: H2 Blockade: H2 receptor antagonist + exercise training; H2 blockade: oral blockade Exercise training: Interval-based cycling exercise

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Change in muscle transcriptome (direct response)	RNA-sequencing	On each test-day: 0 minutes after the exercise training versus at rest.
Change in muscle transcriptome (delayed response)	RNA-sequencing	On each test-day: 180 minutes after the exercise training versus at rest.
Muscle glycogen depletion	Fluorometric determination of muscle glycogen levels	On each test-day: 0 minutes after the exercise training versus at rest.
Muscle glycogen resynthesis	Fluorometric determination of muscle glycogen levels	On each test-day: 180 minutes after the exercise training versus at rest.
Plasma volume change	Plasma volume based on hemoglobin and hematocrit concentration.	Change from rest to different time-points after exercise (0, 30, 60, 120 and 180 minutes after the exercise training).

Secondary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Heart rate during the exercise training	Heart rate during the exercise training.	Continuously during training on each test-day.
Blood lactate	Capillary lactate concentration.	On each test-day at 10 time-points: at rest, during exercise and 0, 30, 60, 120 and 180 minutes after the exercise training.
Blood glucose	Capillary glucose concentration.	On each test-day at 10 time-points: at rest, during exercise and 0, 30, 60, 120 and 180 minutes after the exercise training.
Blood histamine	histamine concentration in blood samples.	On each test-day at 6 time-points: at rest and 0, 30, 60, 120 and 180 minutes after the exercise training.
Blood insulin	insulin concentration in blood samples.	On each test-day at 6 time-points: at rest and 0, 30, 60, 120 and 180 minutes after the exercise training.
Muscle signaling pathways relevant for glucose metabolism and cardiovascular health	Phosphorylation status of proteins assessed by Western Blotting	On each test-day: at rest, 0 minutes and 180 minutes after the exercise training.

Collaborators and Investigators

• Sponsor: University Ghent
• Collaborators: Research Foundation Flanders
• Investigators: Principal Investigator: Wim Derave, Professor, University Ghent

Study record dates

Study Major Dates

• Study Start (Actual): August 16, 2021
• Primary Completion (Actual): December 6, 2021
• Study Completion (Actual): December 31, 2021

Study Registration Dates

• First Submitted: October 15, 2021
• First Submitted That Met QC Criteria: November 11, 2021
• First Posted (Actual): November 23, 2021

Study Record Updates

• Last Update Posted (Actual): April 12, 2024
• Last Update Submitted That Met QC Criteria: April 11, 2024
• Last Verified: April 1, 2024

More Information

Terms related to this study

• Additional Relevant MeSH Terms: Physiological Effects of Drugs; Neurotransmitter Agents; Molecular Mechanisms of Pharmacological Action; Histamine Antagonists; Histamine Agents; Histamine H1 Antagonists; Histamine H2 Antagonists
• Other Study ID Numbers: AET - BC-10237

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: No
• Studies a U.S. FDA-regulated device product: No