Impact of Exercise Combined With Inulin Propionate Ester on Fat Oxidation (IPE)

Impact of Moderate Intensity Exercise Training Combined With Inulin Propionate Ester Supplementation on Whole Body Resting Fat Oxidation and Body Weigh in Overweight Women

There is evidence to suggest that responsiveness to exercise training programmes can be expected to be influenced by changes in resting fat oxidation, an important factor in the aetiology of obesity. Our previous work has shown that oral supplementation with inulin propionate ester (IPE) reduces intra-abdominal fat and prevents weight gain and that oral propionate intake enhances resting fat oxidation. The effects of IPE combined with exercise training on resting fat oxidation and body fatness are unknown. The aim of this study was to investigate the impact of 4-weeks IPE supplementation, in combination with a moderate intensity exercise training programme, on whole body fat oxidation and on plasma GLP-1 and PYY.

Study Overview

• Status: Completed
• Conditions: Exercise; Inulin Propionate Ester
• Intervention / Treatment: Behavioral: Exercise training with Inulin Propionate Ester; Behavioral: Exercise training with placebo

Detailed Description

Participants: Healthy overweight females with BMI >25 kg/m2 and 25-45 years of age were recruited through advertisements and word of mouth on the campus of the University of Glasgow or from other public places. Participants were required to be sedentary, non-smokers, with stable body weight for two months prior to the study enrollment, not pregnant, free of medication, nutritional supplementation or following any specific diet and with no antibiotic use for the past three months. Participants with chronic illness, eating disorders and history of gastrointestinal operations were excluded.

Study Design: This is a randomized study with a parallel design. Study participants underwent 4 week supervised moderate intensity exercise training combined either with IPE (EX/IPE) or cellulose as placebo (EX/Placebo) supplementation at doses of 10g / day. Supplements were provided as sachets containing white powder and participants were asked to consume one sachet per day with breakfast in a way convenient for each participant. Before the start of the study and at the end of the 4-week intervention, participants were asked to conduct a submaximal exercise test. Prior to the first and after the second submaximal exercise test, participants of both groups conducted a 7-hour experimental trial which involved collection of expired air and blood samples in fasted and postprandial states. Body weight and body composition were measured in the fasted state. Prior to the first 7-hour experimental trial, participants were asked to record their diet for 3 days and replicate this intake prior to the second 7-hour experimental trial.

• Study Type: Interventional
• Enrollment (Actual): 20
• Phase: Not Applicable

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 23 years to 43 years (Adult)
• Accepts Healthy Volunteers: No
• Genders Eligible for Study: Female

Description

Inclusion Criteria:

Healthy, sedentary and overweight females with BMI >25 kg/m2 and stable body weight for two months prior to the study.

Exclusion Criteria:

Acute or chronic illness, eating disorders, history of gastrointestinal operations, smoking, pregnancy, taking supplements, and being on specific diet for the past three months.

Study Plan

How is the study designed?

Design Details

• Primary Purpose: Basic Science
• Allocation: Randomized
• Interventional Model: Parallel Assignment
• Masking: None (Open Label)

Number of Arms

2

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
Experimental: Exercise Training with Inulin Propionate Ester; Study participants underwent 4 week supervised moderate intensity exercise training combined with Inulin Propionate Ester supplementation at doses of 10g / day.	Behavioral: Exercise training with Inulin Propionate Ester; Supervised moderate intensity exercise training combined with Inulin Propionate Ester supplementation conducted for 4 weeks.
Experimental: Exercise Training with Placebo; Study participants underwent 4 week supervised moderate intensity exercise training combined with cellulose as placebo at doses of 10g / day.	Behavioral: Exercise training with placebo; Supervised moderate intensity exercise training combined with placebo conducted for 4 weeks

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Resting fat oxidation	Changes in resting fat oxidation measured in the fasted and postprandial states by means of indirect calorimetry (Oxycon Pro, Germany).	4 weeks
Resting carbohydrate oxidation	Changes in resting fat oxidation measured in the fasted and postprandial states by means of indirect calorimetry (Oxycon Pro, Germany).	4 weeks
Metabolic rate	Changes in resting energy expenditure measured in the fasted and postprandial states by means of indirect calorimetry (Oxycon Pro, Germany).	4 weeks

Secondary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Body weight	Changes in body weight measured by TANITA scales (TBF-300).	4 weeks
Body fat (%)	Changes in body fat expressed as percentage (proportion of fat to the total body weight) measured by TANITA scales (TBF-300).	4 weeks
Maximal oxygen consumption [Time Frame: 4 weeks]	Changes in maximal oxygen consumption (ml/kg/min) measured by Servomex Gas Analyser.	4 weeks
Peptide YY (PYY)	Changes in plasma concentration of fasting and postprandial PYY measured using ELISA (Merc Millipore) kit.	4 weeks
Glucagon-like peptide-1 (GLP-1)	Changes in plasma concentration of fasting and postprandial GLP-1 measured using ELISA (Merc Millipore) kit.	4 weeks
Height	Height (meters)measured by stadiometer, a vertical ruler with a sliding horizontal paddle which is adjusted to rest on the top of the head.	4 weeks

Collaborators and Investigators

• Sponsor: University of Glasgow
• Investigators: Principal Investigator: Douglas Morrison, PhD, University of Glasgow

Publications and helpful links

General Publications

• Malkova D, Polyviou T, Rizou E, Gerasimidis K, Chambers ES, Preston T, Tedford MC, Frost G, Morrison DJ. Moderate intensity exercise training combined with inulin-propionate ester supplementation increases whole body resting fat oxidation in overweight women. Metabolism. 2020 Mar;104:154043. doi: 10.1016/j.metabol.2019.154043. Epub 2019 Nov 29.

Study record dates

Study Major Dates

• Study Start (Actual): February 5, 2015
• Primary Completion (Actual): March 10, 2017
• Study Completion (Actual): June 11, 2017

Study Registration Dates

• First Submitted: July 5, 2019
• First Submitted That Met QC Criteria: July 9, 2019
• First Posted (Actual): July 11, 2019

Study Record Updates

• Last Update Posted (Actual): July 11, 2019
• Last Update Submitted That Met QC Criteria: July 9, 2019
• Last Verified: July 1, 2019

More Information

Terms related to this study

• Keywords: Fat oxidation; Gut hormones; Exercise,; Inulin Propionate Ester; Body weigh; Overweight women
• Other Study ID Numbers: UGlasgow IPE; BB/L004259/1 (Other Grant/Funding Number: BBSRC)

Plan for Individual participant data (IPD)

• Plan to Share Individual Participant Data (IPD)?: No

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: No
• Studies a U.S. FDA-regulated device product: No