- ICH GCP
- US Clinical Trials Registry
- Clinical Trial NCT06255509
Dengue Virus Infection Amongst Patients With Acute Febrile Illness
Dengue Virus Infection Amongst Patients With Acute Febrile Illness: Clinical Characteristics, Molecular and Serological Profiles for Vaccine Trial and Roll-out
Study Overview
Status
Conditions
Detailed Description
Dengue is a significant public health problem in Indonesia, with reported cases varies between 25.000-79.000 each year in 2013-2022. The disease is caused by four serotypes of Dengue virus (DENV-1, DENV-2, DENV-3, and DENV-4), transmitted primarily by the Aedes aegypti. However, dengue infection of DEN-4 are rarely reported compared to other dengue serotypes, such as in Vietnam, Philippines, and other Asian or other Latin American countries.
In Indonesia, DEN-4 was also the least serotypes reported. In contrast, DEN-4 is often found during dengue outbreaks. This findings has been reported in Jakarta and Jember outbreaks reports. The low prevalence of DEN-4 maybe because most studies was conducted in hospitals. Also, NS-1 RDT that is used for test has low sensitivity for DEN-4, particularly in secondary infection.
Overcoming the limitation the current diagnostic methods is critical for quick and precise treatment for individuals with dengue infection. Particularly, in the context for vaccine development and clinical trial. We chose Tangerang because it has a high risk of dengue virus transmission due to it is heavily populated area. Dengue remain a significant health problem as 50-100 cases reported each year from Public Health Centers, and >200 patients were hospitalized due to Dengue infection.
Therefore, this study will be conducted at 2 Public Health Centers (Kelapa Dua and Bojong Nangka) and Tangerang District Hospital for 1 year starting from 2 February 2024 to 31 January 2025.
Patient will be recruited when they satisfy inclusion and exclusion criteria and will be tested for RDT test and hematologic test. Blood and clinical data will be obtained from enrolled participant on enrollment and maximum 2 weeks after enrollment. Acute specimens from enrollment will be tested for NS1, IgM/ IgG using ELISA and molecular by PRNT while the convalescent specimen will be tested for IgG and IgM only. A subset of positive cases by PCR will be cultured and tested for PRNT.
Acute specimen from undiagnosed dengue infection will be tested for other pathogens.
Clinical and laboratory data will be analyzed for proportion for dengue among acute fever, proportion for each dengue serotypes, and clinical manifestation for each serotypes will be compared for severity.
Study Type
Enrollment (Estimated)
Contacts and Locations
Study Locations
-
-
Banten
-
Tangerang, Banten, Indonesia, 15111
- Recruiting
- Tangerang District Hospital
-
Contact:
- Dewi Lokida, Clinical Pathologist
- Phone Number: +62816620605
- Email: lokidadewi@yahoo.com
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Contact:
- Atindriya Iswari, MD
- Phone Number: 081211025918
- Email: atindriyatriiswari@gmail.com
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Principal Investigator:
- Dewi Lokida, Clinical Pathologist
-
Principal Investigator:
- Herman Kosasih, PhD
-
Principal Investigator:
- Achmad Muchlis, MD
-
-
Participation Criteria
Eligibility Criteria
Ages Eligible for Study
- Child
- Adult
- Older Adult
Accepts Healthy Volunteers
Sampling Method
Study Population
Description
Inclusion Criteria:
- Patients age ≥ 4 years old with fever (≥ 37.5°C), history of fever with antipyretic use, lasting less than or equal to 7 days
Presence at least one of the following dengue-like symptoms:
- Headache
- Retro-orbital pain
- Nausea/ vomiting
- Muscle, bone, or joint pain
- Rash or bleeding manifestations
- Patient or patient's guardian giving consent to participate in the study
Exclusion Criteria:
- Have a condition that may interfere with study procedures and compliance (based on doctor's assessment)
- Suspected of focal infection
Study Plan
How is the study designed?
Design Details
What is the study measuring?
Primary Outcome Measures
Outcome Measure |
Measure Description |
Time Frame |
---|---|---|
clinical characteristics
Time Frame: 1 year
|
fever (yes/no), headache (yes/no), retro-orbital pain(yes/no), nausea/ vomiting (yes/no), arthralgia/ myalgia (yes/no), bleeding manifestation (yes/no)
|
1 year
|
haematologic profile
Time Frame: 1 year
|
leukosit absolute (number/ ul), platelet (number/ul)
|
1 year
|
Rapid test for dengue
Time Frame: 1 year
|
NS1 (positive/negative), IgM (positive/negative), IgG (positive/negative)
|
1 year
|
molecular (PCR)
Time Frame: 1 year
|
DEN-1 (positive/negative), DEN-2 (positive/negative), DEN-3 (positive/negative), DEN-4 (positive/negative)
|
1 year
|
PRNT
Time Frame: 1 year
|
DEN-1 (titer), DEN-2 (titer), DEN-3 (titer), DEN-4 (titer)
|
1 year
|
Secondary Outcome Measures
Outcome Measure |
Measure Description |
Time Frame |
---|---|---|
prevalence of dengue virus infection among patients with acute fever
Time Frame: 1 year
|
prevalence of DEN-1, DEN-2, DEN-3, DEN-4 among patients with acute fever
|
1 year
|
Collaborators and Investigators
Sponsor
Study record dates
Study Major Dates
Study Start (Estimated)
Primary Completion (Estimated)
Study Completion (Estimated)
Study Registration Dates
First Submitted
First Submitted That Met QC Criteria
First Posted (Estimated)
Study Record Updates
Last Update Posted (Estimated)
Last Update Submitted That Met QC Criteria
Last Verified
More Information
Terms related to this study
Keywords
Additional Relevant MeSH Terms
Other Study ID Numbers
- 445/068/KEP-RSUTNG
Drug and device information, study documents
Studies a U.S. FDA-regulated drug product
Studies a U.S. FDA-regulated device product
This information was retrieved directly from the website clinicaltrials.gov without any changes. If you have any requests to change, remove or update your study details, please contact register@clinicaltrials.gov. As soon as a change is implemented on clinicaltrials.gov, this will be updated automatically on our website as well.
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