Study on Correlated Factors of Barrier and Immune Function in Stress Related Mucosal Disease (SRMD)
2017年6月25日 更新者：Xiuli Zuo
Study on Changes of Barrier and Immune Function in Stress Related Mucosal Disease (SRMD)
To study the changes of barrier and immune function in Stress Related Mucosal Disease (SRMD).
研究概览
详细说明
38 patients admitted to ICU and 15 healthy volunteers were successively enrolled in this study.The healthy volunteers were treated with endoscopy and biopsy .The 38 patients were treated with bedside gastroscopy and diagnosed with SRMD due to illness.
The acute physiology and chronic health evaluation（APACHE）II score were evaluated, malondialdehyde (MDA) as an index of oxygen radical-lipid peroxidation was assessed in the serum.
Transforming growth factor(TGF)β1,claudin3, interleukin (IL) 1 and interleukin（IL）10 levels in the gastric mucosa and plasma level of superoxide dismutase(SOD)，Ischemic modified albumin(IMA)and C-reactive protein（CRP）were evaluated.
研究类型
介入性
注册 (实际的)
53
阶段
- 不适用
联系人和位置
本节提供了进行研究的人员的详细联系信息，以及有关进行该研究的地点的信息。
学习地点
-
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Shandong
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Jinan、Shandong、中国、250012
- Qilu Hospital, Shandong University
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参与标准
研究人员寻找符合特定描述的人，称为资格标准。这些标准的一些例子是一个人的一般健康状况或先前的治疗。
资格标准
适合学习的年龄
18年 至 80年 (成人、年长者)
接受健康志愿者
是的
有资格学习的性别
全部
描述
Inclusion Criteria:
- Occurrence time: ≤72h.
- With complete clinical data.
- Patients admitted to ICU were treated with bedside endoscopy and diagnosed with SRMD.
Exclusion Criteria:
- Primary digestive tract diseases included stomach cancer, gastric ulcer, cirrhosis with esophagus fundus ventricularis varices and esophageal cancer.
- Patients were complicated with gastric perforation.
- Nonsteroidal anti-inflammatory drugs and antiplatelet agents had been used recently, or with the history of major surgery.
学习计划
本节提供研究计划的详细信息，包括研究的设计方式和研究的衡量标准。
研究是如何设计的？
设计细节
- 主要用途：其他
- 分配：不适用
- 介入模型：单组作业
- 屏蔽：无（打开标签）
武器和干预
参与者组/臂 |
干预/治疗 |
|---|---|
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其他：Endoscopy biopsy and blood
The healthy volunteers were treated with endoscopy biopsy and blood.The patients were treated with bedside endoscopy biopsy and diagnosed with SRMD due to illness，then collect their blood samples.
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Medical operation
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研究衡量的是什么？
主要结果指标
结果测量 |
措施说明 |
大体时间 |
|---|---|---|
|
malondialdehyde (MDA)（nmol/mL）
大体时间：Month 1
|
MDA is an index of oxygen radical-lipid peroxidation was assessed in the serum。Thiobarbituric acid （TBA）method was applied for MDA.
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Month 1
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superoxide dismutase(SOD)(u/mL)
大体时间：Month 1
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The activity of superoxide dismutase(SOD) reflects the the antioxidant ，capacity.Pyrogallol substrate method was applied for SOD.
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Month 1
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claudin3
大体时间：Month 1
|
Tight junctions (TJs) are the most apical component of the intercellular junctional complexes.
TJs consist of occludin, the claudin family of proteins, and junctional adhesion molecule (JAM).Among these TJ molecules, claudins are major integral membrane proteins of TJ strands.Studies have shown that H2O2 can change the number of claudin-3.Previously in vitro experiments have reported that H2O2 increases the permeability of the epithelial monolayer by splitting the cell-tight junction complex structure.
In our study,the claudin3 in gastric mucosa was measured by western blot method.
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Month 1
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TGFβ1
大体时间：Month 2
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Measurement of TGFβ1 in gastric mucosa by immunohistochemistry method and microscopic observation.Image-Pro Plus 6.0 software was used to select the same brownish yellow as a uniform standard for determining the positiveness of all photographs.
Each photo was analyzed to determine the cumulative cumulative optical density of each photograph.We may collect serm to test TGFβ1 using ELISA method，or we may omit this step.
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Month 2
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Acute physiology and chronic health evaluation（APACHE）II score
大体时间：Month 3
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Acute physiology and chronic health evaluation（APACHE）II score was completed within 24h after admitting, including 12 physiological parameters (each item 0-4 points, total score 0-60 points), age (0-6 points) and chronic disease health status (2-5 points).
The total score was 0-71 points.
The higher the score, the more severe the illness will be.
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Month 3
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interleukin（IL）1β
大体时间：month 4
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Measurement of interleukin（IL）1β in gastric mucosa by immunohistochemistry method.Image-Pro Plus 6.0 software was used to select the same brownish yellow as a uniform standard for determining the positiveness of all photographs.
Each photo was analyzed to determine the cumulative cumulative optical density of each photograph.We may collect serm to test IL1β using ELISA method，or we may omit this step.
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month 4
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interleukin（IL）10
大体时间：Month 5
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We measure interleukin（IL）10 in gastric mucosa by immunohistochemistry method.Image-Pro Plus 6.0 software was used to select the same brownish yellow as a uniform standard for determining the positiveness of all photographs.
Each photo was analyzed to determine the cumulative cumulative optical density of each photograph.We may collect serm to test IL10 using ELISA method，or we may omit this step.
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Month 5
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Nitric oxide synthasei（iNOS）
大体时间：Month 6
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Measurement of inducible nitric oxide synthasei（iNOS ）in gastric mucosa by immunohistochemistry method.Image-Pro Plus 6.0 software was used to select the same brownish yellow as a uniform standard for determining the positiveness of all photographs.
Each photo was analyzed to determine the cumulative cumulative optical density of each photograph.
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Month 6
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次要结果测量
结果测量 |
措施说明 |
大体时间 |
|---|---|---|
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Ischemic modified albumin（IMA）(u/ml)
大体时间：Month 7
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Ischemic modified albumin(IMA) is a candidate marker for assessing myocardial ischemia which has been identified as a marker of oxidative stress in a number of studies.Free cobalt colorimetric method was applied for IMA.
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Month 7
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C-reactive protein（CRP）
大体时间：Month 8
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Latex immunoturbidimetric method was applied for C-reactive protein（CRP）.
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Month 8
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合作者和调查者
在这里您可以找到参与这项研究的人员和组织。
赞助
调查人员
- 首席研究员：Xiuli Zuo, PhD、Qilu Hospital Of Shandong University
研究记录日期
这些日期跟踪向 ClinicalTrials.gov 提交研究记录和摘要结果的进度。研究记录和报告的结果由国家医学图书馆 (NLM) 审查，以确保它们在发布到公共网站之前符合特定的质量控制标准。
研究主要日期
学习开始 (实际的)
2016年1月1日
初级完成 (实际的)
2017年4月1日
研究完成 (实际的)
2017年4月1日
研究注册日期
首次提交
2017年5月9日
首先提交符合 QC 标准的
2017年6月25日
首次发布 (实际的)
2017年6月27日
研究记录更新
最后更新发布 (实际的)
2017年6月27日
上次提交的符合 QC 标准的更新
2017年6月25日
最后验证
2017年5月1日
更多信息
与本研究相关的术语
关键字
其他研究编号
- 2015SDU-QILU-G006
计划个人参与者数据 (IPD)
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不
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