Neuroproprioceptive "Facilitation, Inhibition" and Brain Plasticity (NEFAI)

实验性的：运动程序激活疗法

MPAT 是由我们的团队开发和验证的方法。 在这种疗法中，患者被矫正为关节功能居中的姿势。 然后应用体感（手动和口头）刺激来激活大脑中的运动程序，然后导致患者在躺下、坐下、站立或向前移动时整个身体的共同收缩。 在各种条件下，在不同的情况和环境下重复激活的程序，以教会患者在日常生活中自动使用获得的运动技能。 治疗是在布拉格 Kralovske Vinohrady 大学医院神经科的门诊区域内实现的。

行为的：Motor program activating therapy

Pacients underwent two months' therapy consisted of 16 face-to-face sessions (1 hour, twice a week for two months).

实验性的：Vojta 的反射运动

VRL 是捷克共和国 MS 患者的标准方法。 在治疗中，通过刺激特定区域激活反射运动的整体模式，将个体置于精确确定的初始位置（仰卧、俯卧和侧卧、低跪位置）。 这些运动模式具有向前运动（运动）的特性，并且运动响应是精确定义的。 反射运动（反射转动和反射爬行）在治疗中用于激活肌肉功能的非自愿反应，这是自发运动所必需的。 治疗是在莫托尔大学医院的康复和运动医学系实现的。

行为的：Vojta's reflex locomotion

Pacients underwent two months' therapy consisted of 16 face-to-face sessions (1 hour, twice a week for two months).

实验性的：Functional electric stimulation

Functional electric stimulation in the postural corrected position was developed at our workplace. Participants first underwent individual two-hour session consisting of postural correction using MPAT and the device (The WalkAide® System, Innovative Neurotronics Inc., 4999 Aircenter Circle, Suite 103 Reno, NV 89502, USA) programming (28). Patients received the device to use as much as they felt they were able to during their normal daily living activities thereafter.

行为的：Functional electric stimulation

Pacients uderwent two months' therapy. They used the whole time Functional electric stimulation during activities of daily living and underwent 2 individual sessions of Motor program activating therapy.

White Matter Integrity

Magnetic resonance imaging on a 3T magnetic resonance scanner (Siemens Trio Tim, Erlangen, Germany) using a 12-channel phased-array head coil. A diffusion tensor was fitted to each voxel of the brain, and a fractional anisotropy (FA) map was created for each subject. The images were further analyzed using tract-based spatial statistics (TBSS).

Fractional anisotropy (FA) is a scalar value between zero and one that describes the degree of anisotropy of a diffusion process. A value of zero means that diffusion is isotropic, i.e. it is unrestricted (or equally restricted) in all directions. A value of one means that diffusion occurs only along one axis and is fully restricted along all other directions. FA is a measure often used in diffusion imaging where it is thought to reflect fiber density, axonal diameter, and myelination in white matter. The FA is an extension of the concept of eccentricity of conic sections in 3 dimensions, normalized to the unit range.

Berg Balance Scale, BBS

14 items objective measure of static balance and risk of falls (0 the best, 56 the worse)

Timed up and go Test, TUG

time necessary to stand up, go 3 meters, turn around, go back and sit to chair (longer time in seconds is worse function)

the 12-item Multiple Sclerosis Walking Scale, MSWS - 12

The Multiple Sclerosis Walking Scale is a self-assessment scale which measures the impact of MS on walking. It consists of 12 questions concerning the limitations to walking due to MS during the past 2 weeks. Each item can be answered with 5 options, with 1 meaning no limitation and 5 extreme limitation.

A total score can be generated and transformed to a 0 to 100 scale by subtracting the minimum score possible (12) from the patient's score, dividing by the maximum score possible minus the minimum possible (60-12 or 48), and multiplying the result by 100. Walking improvement on the MSWS-12 is indicated by negative change scores.

29 项多发性硬化影响量表，MSIS -29

一个包含 29 个项目的自我报告测量，其中 20 个项目与物理量表相关，9 个项目与心理量表相关。 项目询问过去两周 MS 对日常生活的影响。 所有项目都有 5 个反应选项：1“完全没有”到 5“非常”。 两个量表中的每一个都通过对项目的反应求和，然后转换为 0-100 的量表进行评分，其中 100 表示疾病对日常功能的影响更大（健康状况更差）。