- ICH GCP
- US Clinical Trials Registry
- Clinical Trial NCT00501774
A Search for Helicobacter Pylori in Localized Vulvodynia
A Laboratory and Clinical Search for the Presence of H. Pylori and Treatment Against it in Localized Vulvodynia (Vestibulitis, Vestibulodynia).
Study Overview
Status
Conditions
Detailed Description
Subsequent sections from the paraffin blocks were prepared and stained by modified Giemsa. Immunostaining for H. pylori was done as described. In short, tissue sections were deparaffinized in xylene, rehydrated through decreasing concentrations of alcohol ending in phosphate-buffered saline (PBS), and subjected to pretreatment with Proteinase K (8 minutes). The sections were quenched with 3% hydrogen peroxidase, incubated with protein block for 15 minutes at room temperature, and washed in PBS. Tissues then were incubated with polyclonal rabbit anti-H pylori antibody (dilution, 1:10; clone ch-20 429, DAKO, Carpinteria, CA). Finally, sections were washed in 0.05% polysorbate 20 in PBS, pH 7.4, and the bound antibody was detected using streptavidin and biotinylated secondary antibody with diaminobenzidine as the chromogen. Sections were counterstained with hematoxylin, dehydrated, and mounted. Negative controls were sections treated as above, but instead of incubation with the primary antibody, they were incubated with 1% bovine serum albumin in PBS.
Vulvar biopsies of seven other women without localized vulvodynia served as healthy controls.
The positive and negative control gastric tissues for the immunohistochemical stain of the H. pylori microorganisms were obtained from the archives of the Department of Pathology.
Study Type
Enrollment (Actual)
Contacts and Locations
Study Locations
-
-
-
Haifa, Israel
- Pathology Laboratory
-
-
Participation Criteria
Eligibility Criteria
Ages Eligible for Study
Accepts Healthy Volunteers
Genders Eligible for Study
Description
Inclusion Criteria:
- Paraffin blocks of vestibular tissues from patients with Vestibulodynia
Exclusion Criteria:
- Patients without vestibulodynia
Study Plan
How is the study designed?
Design Details
- Observational Models: Case-Control
- Time Perspectives: Other
Collaborators and Investigators
Investigators
- Principal Investigator: Jacob Bornstein, MD, westen Galilee Hospital
Publications and helpful links
General Publications
- Harlow BL, Stewart EG. A population-based assessment of chronic unexplained vulvar pain: have we underestimated the prevalence of vulvodynia? J Am Med Womens Assoc (1972). 2003 Spring;58(2):82-8.
- Gray SF, Wyatt JI, Rathbone BJ. Simplified techniques for identifying Campylobacter pyloridis. J Clin Pathol. 1986 Nov;39(11):1279. doi: 10.1136/jcp.39.11.1279-a. No abstract available.
Study record dates
Study Major Dates
Study Start
Study Completion (Actual)
Study Registration Dates
First Submitted
First Submitted That Met QC Criteria
First Posted (Estimate)
Study Record Updates
Last Update Posted (Estimate)
Last Update Submitted That Met QC Criteria
Last Verified
More Information
Terms related to this study
Additional Relevant MeSH Terms
Other Study ID Numbers
- Sabo-1
This information was retrieved directly from the website clinicaltrials.gov without any changes. If you have any requests to change, remove or update your study details, please contact register@clinicaltrials.gov. As soon as a change is implemented on clinicaltrials.gov, this will be updated automatically on our website as well.
Clinical Trials on Vulvodynia
-
University Hospital, GhentCompletedLocalized Provoked VulvodyniaBelgium
-
Oregon Health and Science UniversityMedical Research Foundation, OregonCompleted
-
Meir Medical CenterCompleted
-
McLean Center for Complementary and Alternative...UnknownVulvodynia | Vulvar Vestibulitis | Vulvodynia, Generalized | Vulvar PainUnited States
-
Uppsala UniversityCompletedVulvodynia, Generalized | Provoked VestibulodyniaSweden
-
Meir Medical CenterCompletedLocalized Provoked VulvodyniaIsrael
-
University of Illinois at ChicagoUniversity of FloridaCompletedVulvodynia | Vulvar Vestibulitis | Vulvodynia, GeneralizedUnited States
-
Colleen StockdaleTerminatedVulvar Pain Symptoms | Vulvodynia (Chronic Vulvar Pain)United States
-
Oregon College of Oriental MedicineOregon Health and Science University; National Vulvodynia Association; Council...CompletedVulvodynia | Vulvar Vestibulitis | Provoked Vestibulodynia | Provoked, Localized VulvodyniaUnited States
-
University of TennesseeUniversity of Tennessee Health Science CenterCompleted