Effect of an Exercise on Serum Uncoupling Protein-1 Level and the Role of Uncoupling Protein-1-3826 A/G Polymorphism

The Effect of an Acute Maximal Exercise on Serum Uncoupling Protein-1 Level and the Role of Uncoupling Protein-1-3826 A/G Polymorphism

This study aims to determine the effect of an acute maximal exercise on serum uncoupling protein-1, irisin, interleukin 6 levels, and on blood lipid and lipoprotein concentrations. This study also evaluates the role of uncoupling protein-1-3826 A/G polymorphism on this eventual effect.

Therefore, this study hypothesizes that:

H01: Sedentary people and athletes' serum uncoupling protein 1, irisin, and interleukine 6 levels are different before and after an acute maximal exercise.

H02: Sedentary people and athletes' basal serum uncoupling protein 1 levels are associated with uncoupling protein 1-3826 A/G polymorphism.

H03: Uncoupling protein 1-3826 A/G polymorphism has a modifying role in the effect of maximal exercise on serum uncoupling protein 1 levels.

H04: Sedentary people and athletes' basal serum lipid and lipoprotein levels are associated with uncoupling protein 1-3826 A/G polymorphism.

H05: Uncoupling protein 1-3826 A / G polymorphism has a modifying role in the effect of maximal exercise on serum lipid and lipoprotein levels.

Study Overview

• Status: Completed
• Conditions: Polymorphism, Restriction Fragment Length; Irisin; Uncoupling Protein-1
• Intervention / Treatment: Other: Yo-Yo intermittent recovery test (level 1)

• Study Type: Interventional
• Enrollment (Actual): 86
• Phase: Not Applicable

Contacts and Locations

Study Locations

• Turkey
  • Izmir, Turkey
    • Ege University Faculty of Sport Sciences

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 18 years to 35 years (Adult)
• Accepts Healthy Volunteers: Yes
• Genders Eligible for Study: Male

Description

Inclusion Criteria:

• Athletes group:

  • Must be basketball, or volleyball, or handball players.
  • Must be subjects who have been training regularly for at least 3 months.

• Sedentary group:

  * Must be subjects who have not been training regularly for at least 3 months.

• For both groups:

  • Healthy.
  • Non obese (body mass index < 30).
  • Aged between 18 and 35 years.

Exclusion Criteria:

• For both groups:

  • Being regular consumer of cigarettes, alcohol, drugs and antioxidant substances.
  • Being obese (body mass index > 30).
  • Having an acute or chronic injury which is limiting physical performance.
  • Having a disease (anemia, infection...) detected from the biochemical analysis of the blood samples.
  • Being injured or sick during the study.

Study Plan

How is the study designed?

Design Details

• Primary Purpose: Basic Science
• Allocation: Non-Randomized
• Interventional Model: Parallel Assignment
• Masking: None (Open Label)

Number of Arms

2

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
Experimental: Athletes Group; The athletes group will be composed of healthy, non obese (body mass index < 30), male basketball, volleyball and handball players aged between 18 and 35 years who have been training regularly for at least 3 months. Participants must not be regular consumer of cigarettes, alcohol, drugs and antioxidant substances. Intervention: Yo-Yo intermittent recovery test (level 1) is administered.	Other: Yo-Yo intermittent recovery test (level 1); The Yo-Yo intermittent recovery test (level 1) will be administered once.
Experimental: Sedentary Group; The sedentary group will be composed of healthy, non obese (body mass index < 30), male aged between 18 and 35 years who have not been training regularly for at least 3 months. Participants must not be regular consumer of cigarettes, alcohol, drugs and antioxidant substances. Intervention: Yo-Yo intermittent recovery test (level 1) is administered.	Other: Yo-Yo intermittent recovery test (level 1); The Yo-Yo intermittent recovery test (level 1) will be administered once.

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Change from the baseline serum uncoupling protein 1 level at 3 minutes post Yo-Yo intermittent recovery test (level 1)	Volunteers' postprandial venous blood samples were taken 15 minutes before the Yo-Yo intermittent recovery test (level 1) and 3 minutes post test. Uncoupling protein 1 analyzes were performed in the laboratory.	15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1)
Change from the baseline serum irisin level at 3 minutes post Yo-Yo intermittent recovery test (level 1)	Volunteers' postprandial venous blood samples was taken 15 minutes before the Yo-Yo intermittent recovery test (level 1) and 3 minutes post test. Irisin analyzes were performed in the laboratory.	15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1)
Uncoupling protein-1-3826 A/G polymorphism determination	Volunteers' postprandial venous blood samples were taken 15 minutes before the Yo-Yo intermittent recovery test (level 1). Polymorphism was determined in the laboratory.	15 minutes before the Yo-Yo intermittent recovery test (level 1)

Secondary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Change from the baseline serum interleukin-6 level at 3 minutes post Yo-Yo intermittent recovery test (level 1)	Volunteers' postprandial venous blood samples was taken 15 minutes before the Yo-Yo intermittent recovery test (level 1) and 3 minutes post test. Interleukine-6 analyzes were performed in the laboratory.	15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1)
Change from the baseline serum nitric oxide level at 3 minutes post Yo-Yo intermittent recovery test (level 1)	Volunteers' postprandial venous blood samples was taken 15 minutes before the Yo-Yo intermittent recovery test (level 1) and 3 minutes post test. Nitric oxide analyzes were performed in the laboratory.	15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1)

Other Outcome Measures

Outcome Measure	Measure Description	Time Frame
Change from the baseline serum C reactive protein level at 3 minutes post Yo-Yo intermittent recovery test (level 1)	Volunteers' postprandial venous blood samples was taken 15 minutes before the Yo-Yo intermittent recovery test (level 1) and 3 minutes post test. C reactive protein analyzes were performed in the laboratory.	15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1)
Change from the baseline serum creatine kinase level at 3 minutes post Yo-Yo intermittent recovery test (level 1)	Volunteers' postprandial venous blood samples was taken 15 minutes before the Yo-Yo intermittent recovery test (level 1) and 3 minutes post test. Creatine kinase analyzes were performed in the laboratory.	15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1)
Change from the baseline serum alanine aminotransferase level at 3 minutes post Yo-Yo intermittent recovery test (level 1)	Volunteers' postprandial venous blood samples was taken 15 minutes before the Yo-Yo intermittent recovery test (level 1) and 3 minutes post test. Alanine aminotransferase analyzes were performed in the laboratory.	15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1)
Change from the baseline serum aspartate aminotransferase level at 3 minutes post Yo-Yo intermittent recovery test (level 1)	Volunteers' postprandial venous blood samples was taken 15 minutes before the Yo-Yo intermittent recovery test (level 1) and 3 minutes post test. Aspartate aminotransferase analyzes were performed from serum samples in the Medical Biochemistry Laboratory of the Ege University Faculty of Medicine.	15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1)
Change from the baseline hemogram test at 3 minutes post Yo-Yo intermittent recovery test (level 1)	Volunteers' postprandial venous blood samples was taken 15 minutes before the Yo-Yo intermittent recovery test (level 1) and 3 minutes post test. Hemogram test analyzes were performed in the laboratory.	15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1)
Change from the baseline at serum glucose level at 3 minutes post Yo-Yo intermittent recovery test (level 1)	Volunteers' postprandial venous blood samples was taken 15 minutes before the Yo-Yo intermittent recovery test (level 1) and 3 minutes post test. Glucose analyzes were performed in the laboratory.	15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1)
Change from the baseline serum urea level at 3 minutes post Yo-Yo intermittent recovery test (level 1)	Volunteers' postprandial venous blood samples was taken 15 minutes before the Yo-Yo intermittent recovery test (level 1) and 3 minutes post test. Urea analyzes were performed in the laboratory.	15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1)
Change from the baseline serum creatinine level at 3 minutes post Yo-Yo intermittent recovery test (level 1)	Volunteers' postprandial venous blood samples was taken 15 minutes before the Yo-Yo intermittent recovery test (level 1) and 3 minutes post test. Creatinine analyzes were performed in the laboratory.	15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1)
Change from the baseline serum total cholesterol level at 3 minutes post Yo-Yo intermittent recovery test (level 1)	Volunteers' postprandial venous blood samples was taken 15 minutes before the Yo-Yo intermittent recovery test (level 1) and 3 minutes post test. Total cholesterol analyzes were performed in the laboratory.	15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1)
Change from the baseline serum low density lipoprotein level at 3 minutes post Yo-Yo intermittent recovery test (level 1)	Volunteers' postprandial venous blood samples was taken 15 minutes before the Yo-Yo intermittent recovery test (level 1) and 3 minutes post test. Low density lipoprotein analyzes were performed in the laboratory.	15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1)
Change from the baseline serum high density lipoprotein level at 3 minutes post Yo-Yo intermittent recovery test (level 1)	Volunteers' postprandial venous blood samples was taken 15 minutes before the Yo-Yo intermittent recovery test (level 1) and 3 minutes post test. High density lipoprotein analyzes were performed in the laboratory.	15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1)
Change from the baseline serum triglyceride level at 3 minutes post Yo-Yo intermittent recovery test (level 1)	Volunteers' postprandial venous blood samples was taken 15 minutes before the Yo-Yo intermittent recovery test (level 1) and 3 minutes post test. Triglyceride analyzes were performed in the laboratory.	15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1)
Change from the baseline serum total antioxidant level at 3 minutes post Yo-Yo intermittent recovery test (level 1)	Volunteers' postprandial venous blood samples was taken 15 minutes before the Yo-Yo intermittent recovery test (level 1) and 3 minutes post test. Total antioxidant analyzes were performed in the laboratory.	15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1)
Change from the baseline serum total oxidant level at 3 minutes post Yo-Yo intermittent recovery test (level 1)	Volunteers' postprandial venous blood samples was taken 15 minutes before the Yo-Yo intermittent recovery test (level 1) and 3 minutes post test. Total oxidant analyzes were performed in the laboratory.	15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1)
Change from the baseline heparin plasma thiobarbituric acid reactive substances level at 3 minutes post Yo-Yo intermittent recovery test (level 1)	Volunteers' postprandial venous blood samples was taken 15 minutes before the Yo-Yo intermittent recovery test (level 1) and 3 minutes post test. Thiobarbituric acid reactive substances analyzes were performed in the laboratory.	15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1)

Collaborators and Investigators

• Sponsor: Eastern Mediterranean University
• Collaborators: Ege University
• Investigators: Study Chair: Faruk Turgay, Assoc. Prof, Ege University, Faculty of Sports Science

Study record dates

Study Major Dates

• Study Start (Actual): November 11, 2019
• Primary Completion (Actual): September 2, 2020
• Study Completion (Actual): September 7, 2020

Study Registration Dates

• First Submitted: November 4, 2019
• First Submitted That Met QC Criteria: November 6, 2019
• First Posted (Actual): November 7, 2019

Study Record Updates

• Last Update Posted (Actual): September 9, 2020
• Last Update Submitted That Met QC Criteria: September 7, 2020
• Last Verified: September 1, 2020

More Information

Terms related to this study

• Keywords: Irisin; Athletes; Fat tissue; Beige fat cell; Sedentary people
• Other Study ID Numbers: 18-10.2/56

Plan for Individual participant data (IPD)

• Plan to Share Individual Participant Data (IPD)?: UNDECIDED

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: No
• Studies a U.S. FDA-regulated device product: No