- ICH GCP
- US Clinical Trials Registry
- Clinical Trial NCT04420819
Effects of Ischemic Preconditioning on Muscle Damage Induced by Excentric Exercise
April 19, 2022 updated by: Franciele Marques Vanderle, Paulista University
Effects of Ischemic Preconditioning on Muscle Damage Induced by Excentric Exercise: Randomized Clinical Trial Placebo Controlled
Introduction: eccentric exercise (EE) has been widely used in rehabilitation and in improving physical fitness due to its mechanical advantage and less metabolic demand, however, EE can induce muscle damage providing structural changes and reduced muscle function, therefore, it is necessary to look for alternatives to reduce this damage caused by stress.
Thus, ischemic preconditioning (PCI) can be seen as an aid in reducing the damage caused by EE, as it can attenuate the ischemia-reperfusion injury, and can be used to accelerate the post-exercise recovery process.
Objectives: to compare the effects of PCI, using different occlusion pressures, on acute and late responses to perceptual outcomes and muscle injury markers, in addition to checking whether the technique causes deleterious effects on performance in post-exercise recovery.
Methods: a randomized placebo controlled clinical trial will be conducted with 80 healthy men aged 18 to 35 years who will be randomly divided into four groups: PCI using total occlusion pressure (POT), PCI with 40% more than POT, placebo (10 mmHg) and control.
The PCI protocol will consist of four cycles of ischemia and reperfusion of five minutes each.
All groups will perform an EE protocol, initial assessments, immediately after the end of the EE, 24, 48, 72 and 96 hours after exercise, with the maximum isometric voluntary contraction (CVIM) assessed by the isokinetic dynamometer, vectors of cell integrity by by means of electrical bioimpedance (BIA), creatine kinase (CK), blood lactate, pain on the visual analog scale (VAS), pain threshold by the pressure algometer, and tone, muscle rigidity and elasticity by myotonometry.
The descriptive statistical method and analysis of variance will be used for the repeated measures model.
The level of significance will be p <0.05.
Study Overview
Study Type
Interventional
Enrollment (Actual)
80
Phase
- Not Applicable
Contacts and Locations
This section provides the contact details for those conducting the study, and information on where this study is being conducted.
Study Locations
-
-
SP
-
Presidente Prudente, SP, Brazil, 55
- Franciele Marques Vanderlei
-
-
Participation Criteria
Researchers look for people who fit a certain description, called eligibility criteria. Some examples of these criteria are a person's general health condition or prior treatments.
Eligibility Criteria
Ages Eligible for Study
18 years to 35 years (ADULT)
Accepts Healthy Volunteers
Yes
Genders Eligible for Study
Male
Description
Inclusion Criteria:
- not having any health condition that contraindicates or prevents EE;
- not having diagnosed diabetes and high blood pressure;
- not having inflammatory, psychiatric, cardiovascular and / or respiratory rheumatic disease;
- not being an alcoholic, using drugs and / or a smoker;
- without a history of knee surgery (for example, meniscal repair and ligament reconstruction) or recent musculoskeletal injury to the lower limbs that may impair performance during tests or interventions (for example, muscle injury, tendinopathy, patellofemoral pain in the lower limbs and / or back pain in the last six months);
- without involvement in any type of training program during the study period;
- not being engaged in the lower limb strength training program during the three months prior to participating in the study;
- not using ergogenic supplements to improve physical performance and / or muscle mass and / or vasoactive drugs;
- not having one or more risk factors predisposing to thromboembolism.
Exclusion Criteria:
- present a health problem that does not allow continuity,
- use medication, electrotherapy or other therapeutic means that may interfere with any result;
- perform unusual or strenuous physical activities during the evaluation period;
- want to leave the study.
Study Plan
This section provides details of the study plan, including how the study is designed and what the study is measuring.
How is the study designed?
Design Details
- Primary Purpose: PREVENTION
- Allocation: RANDOMIZED
- Interventional Model: PARALLEL
- Masking: SINGLE
Arms and Interventions
Participant Group / Arm |
Intervention / Treatment |
---|---|
EXPERIMENTAL: IPC-LOP
this group will carry out the baseline assessments, perform the ischemic preconditioning using exactly the limb occlusion pressure , then perform post-IPC assessments and start the excentric exercise, and the post-exercise assessments will take place immediately after the end of the EE and will be repeated in 24h, 48h, 72h and 96h.
|
The IPC is characterized by the application of brief periods of circulatory occlusion (ischemia) and reperfusion of a limb in the minutes to hours that precede the exercise through the insufflations and deflations of a pressure cuff
|
EXPERIMENTAL: IPC-40%
this group will carry out baseline assessments, perform IPC using 40% more occlusion than LOP, then perform assessments after IPC protocol and start EE, and post exercise assessments will take place immediately after the end of EE and will be repeated in 24h, 48h, 72h and 96h.
|
The IPC is characterized by the application of brief periods of circulatory occlusion (ischemia) and reperfusion of a limb in the minutes to hours that precede the exercise through the insufflations and deflations of a pressure cuff
|
PLACEBO_COMPARATOR: IPC-10mmHg:
this group will perform baseline assessments, perform occlusion-perfusion intervention with 10 mmHg restriction characterizing the placebo, then perform post-IPC assessments and initiate EE, and post-exercise assessments will take place immediately after completion of EE and if will repeat in 24h, 48h, 72h and 96h.
|
The IPC is characterized by the application of brief periods of circulatory occlusion (ischemia) and reperfusion of a limb in the minutes to hours that precede the exercise through the insufflations and deflations of a pressure cuff
|
NO_INTERVENTION: CONTR
this group will carry out the baseline assessments, immediately after starting the EE, and the post-exercise assessments will happen immediately after the end of the EE and will be repeated in 24h, 48h, 72h and 96h.
|
What is the study measuring?
Primary Outcome Measures
Outcome Measure |
Measure Description |
Time Frame |
---|---|---|
Muscle damage
Time Frame: Change from baseline at 96 hours after exercise
|
Plasma CK concentration will be obtained by means of 32 μL of capillary blood collected from the digital pulp.
|
Change from baseline at 96 hours after exercise
|
Blood lactate concentration
Time Frame: Change from baseline at 96 hours after exercise
|
Blood lactate concentration of the participant will be collected 25 ml of blood with an ear lobe capillary
|
Change from baseline at 96 hours after exercise
|
Muscle strength in isokinetic
Time Frame: Change from baseline at 96 hours after exercise
|
Maximal voluntary isometric contraction (MVIC)
|
Change from baseline at 96 hours after exercise
|
Secondary Outcome Measures
Outcome Measure |
Measure Description |
Time Frame |
---|---|---|
Muscle pain
Time Frame: Change from baseline at 96 hours after exercise
|
Assessed using a visual analog scale that ranges from 0 to 10 points, the higher the score, the worse the result.
|
Change from baseline at 96 hours after exercise
|
Pain threshold
Time Frame: Change from baseline at 96 hours after exercise
|
Assessed using pressure algometer equipment, and the pain threshold will be classified according to the cut-off point of 2.55kgf.
Participants will be classified as "sensitive to pain" if they have values below 2.55kgf.
|
Change from baseline at 96 hours after exercise
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Resistance through Bioimpedance analysis (BIA)
Time Frame: Change from baseline at 96 hours after exercise
|
Evaluation of tissue resistance, the higher the value, the worse the result
|
Change from baseline at 96 hours after exercise
|
Reactance through Bioimpedance analysis (BIA)
Time Frame: Change from baseline at 96 hours after exercise
|
Evaluation of tissue reactance, the higher the value, the better the cell integrity
|
Change from baseline at 96 hours after exercise
|
Phase angle through Bioimpedance analysis (BIA)
Time Frame: Change from baseline at 96 hours after exercise
|
Assessment of the phase angle, the higher the value, the better the condition of the participant
|
Change from baseline at 96 hours after exercise
|
Tone muscular through Myotonometry used the MyotonPRO equipment
Time Frame: Change from baseline at 96 hours after exercise
|
Natural Oscillation Frequency [Hz], characterizing Tone of the quadriceps femoris muscle
|
Change from baseline at 96 hours after exercise
|
Stiffness through Myotonometry used the MyotonPRO equipment
Time Frame: Change from baseline at 96 hours after exercise
|
Dynamic Stiffness [N/m] of the quadriceps femoris muscle
|
Change from baseline at 96 hours after exercise
|
Elasticity through Myotonometry used the MyotonPRO equipment
Time Frame: Change from baseline at 96 hours after exercise
|
Mechanical Stress Relaxation Time [ms] featuring muscle elasticity of the quadriceps femoris muscle
|
Change from baseline at 96 hours after exercise
|
Perception of recovery
Time Frame: Change from baseline at 96 hours after exercise
|
Perception of recovery was obtained using a 10-point Likert Scale, with a rating of 1 indicating the feeling "not recovered" and a rating of 10 the feeling "fully recovered"
|
Change from baseline at 96 hours after exercise
|
Collaborators and Investigators
This is where you will find people and organizations involved with this study.
Sponsor
Publications and helpful links
The person responsible for entering information about the study voluntarily provides these publications. These may be about anything related to the study.
Study record dates
These dates track the progress of study record and summary results submissions to ClinicalTrials.gov. Study records and reported results are reviewed by the National Library of Medicine (NLM) to make sure they meet specific quality control standards before being posted on the public website.
Study Major Dates
Study Start (ACTUAL)
April 1, 2021
Primary Completion (ACTUAL)
December 1, 2021
Study Completion (ACTUAL)
April 1, 2022
Study Registration Dates
First Submitted
May 29, 2020
First Submitted That Met QC Criteria
June 3, 2020
First Posted (ACTUAL)
June 9, 2020
Study Record Updates
Last Update Posted (ACTUAL)
April 26, 2022
Last Update Submitted That Met QC Criteria
April 19, 2022
Last Verified
April 1, 2022
More Information
Terms related to this study
Keywords
Additional Relevant MeSH Terms
Other Study ID Numbers
- FCT/UNESP
Plan for Individual participant data (IPD)
Plan to Share Individual Participant Data (IPD)?
NO
Drug and device information, study documents
Studies a U.S. FDA-regulated drug product
No
Studies a U.S. FDA-regulated device product
No
This information was retrieved directly from the website clinicaltrials.gov without any changes. If you have any requests to change, remove or update your study details, please contact register@clinicaltrials.gov. As soon as a change is implemented on clinicaltrials.gov, this will be updated automatically on our website as well.
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