Biologic Augmentation With Mesenchymal Stem Cells in Patients Undergoing Anterior Cruciate Ligament Reconstruction (BMAC)

A Prospective Randomized Trial of Biologic Augmentation With Mesenchymal Stem Cells in Patients Undergoing Anterior Cruciate Ligament Reconstruction

This study will evaluate the efficacy of biologic augmentation of ACL reconstruction with bone marrow derived mesenchymal stem cells as measured by magnetic resonance imaging to detect graft healing and integration. Secondary endpoints will include validated patient reported outcome measures, as well as functional outcome using objective examination findings.

Study Overview

• Status: Enrolling by invitation
• Conditions: Anterior Cruciate Ligament Rupture; Anterior Cruciate Ligament Injury
• Intervention / Treatment: Device: Autologous Bone Marrow Derived Mesenchymal Stem Cells; Other: Sham incision

Detailed Description

Introduction

Anterior cruciate ligament (ACL) tears are a common and devastating injury among young athletes, and re-injury poses significant threats to both successful return to play and long term outcomes. Graft healing during ACL reconstruction (ACLR) is a slow biologic process, that involves graft incorporation into bone tunnel sites, as well as intra-articular graft remodeling . The intra-articular graft remodeling process is known as ligamentization, and occurs in three phases after ACLR. The first phase of early healing occurs between 0 and three months, the second phase occurs between 6 months and one year, and the final phase of maturation occurs after 1 year when the graft appears very similar to the native ACL. The studies that defined these time points biopsied human bone-patella tendon-bone (BTB) ACL autografts at different postoperative points, and described changes occurring over time in regards to vascularization, cellular aspects, and appearance of the extracellular matrix in comparison to control ACLs . Unlike animal models which display a necrotic stage, grafts in human models undergo an early vascular invasion, associated with fibroblasts that are converted to a hyper-cellular matrix with only focalized areas of necrosis . This process occurs as early as 3 weeks postoperatively, and increases over time as areas similar to the native patellar tendon with mature collagen and metabolically quiescent cells decrease . This process is the limiting factor in graft healing for bone-patella tendon-bone (BTB) grafts, as osteointegration occurs earlier .

The processes of osteointegration and ligamentization are responses to the new intrasynovial milieu and physical forces that the new graft is exposed to. During these healing and restructuring processes, failure load and stiffness of grafts decreases by up to 24% at 7 weeks, before strengthening to their potential . This contributes to the long recovery period for ACLR, and the risk of re-rupture during this period. Advances in biologic adjuncts to accelerate and improve this healing process would have a significant impact on the management of ACL injuries.

Recently, biologic preparations of various growth factors have been developed to safely and effectively treat a variety of musculoskeletal conditions, including tendinopathies and arthritis . Bone marrow aspirate concentrate (BMAC) consists of undifferentiated mesenchymal stem cells that are concentrated and applied to the injury site . Previous studies have demonstrated this treatment to offer the pluripotent potential of the cells to impact healing, regeneration, biomechanical strength, and reducing bone-tunnel enlargement . Investigators have demonstrated that mesenchymal stem cell reinforced grafts had a significantly higher failure load and stiffness as early as 8 week post-operation in a rabbit model, suggesting an added benefit of advanced healing . Using BMSCs can offer a novel method to enhance tendon graft osteointegration. While there have been numerous laboratory studies researching the effect of mesenchymal stem cells, there lacks evidence in translation to the clinical setting. Silva et al performed a study where they injected bone marrow derived stem cells (BMSCs) into patellar tendon grafts to examine for increased graft-to-bone tunnel integration. They did not find a significant difference of tunnel integration on MRI imaging, but they did not examine the intra-articular portions for the effect on the ligamentization process, nor did they examine the effect on clinical examination and patient reported outcomes .

Quantitative MRI is a non-invasive method to interrogate tissue properties and evaluate the biochemical composition of tissues. The T2* sequence gives information regarding tissue graft volume, water content, fiber alignment, and tissue density. Median grayscale values (a measure to determine tissue quality)can also be correlated to maximum failure load, yield load, and linear stiffness of grafts in a porcine model. Increases in MRI signals in ACL grafts have been found to be time dependent, becoming well established by three months, particularly at the distal intra-articular portion near the tibial tunnel . These changes have not been shown previously to be predictive of graft failure, but Investigators have recently also confirmed that normalized T2* signal intensity values of ACL grafts have significant correlation with knee instability related to ligamentization and tunnel healing.

A recent study examined intra-articular graft maturation after platelet-rich plasma gel (PRPG) application, with MRI at month intervals from 3-12 months postoperatively. The intra-articular segment was divided in a proximal, middle, and distal segment, on a scale from 0-3 ranging from completely homogenous to severely heterogeneous. The mean time to obtain completely homogenous bone-patella tendon-bone (BTB) graft was 109 days for the PRPG group, and 363 for the control group 8.

We propose using quantitative magnetic resonance (MR) imaging with T2* mapping to investigate if BMAC treatment at time of ACL reconstruction can accelerate the graft maturation and healing process. We will perform a randomized controlled trial of patients undergoing ACL reconstruction with bone-patellar tendon-bone allograft evaluating the T2* weighted MRI changes of the graft at 3 and 9 months after surgical reconstruction. Additionally, we will obtain patient-reported outcome measures from this cohort to evaluate if BMAC treatment imparts a clinically-significant effect on ACL reconstruction.

Study Rationale

This study will evaluate the efficacy of biologic augmentation of ACL reconstruction with BMSCs as measured by magnetic resonance imaging to detect graft healing and integration. Secondary endpoints will include validated patient reported outcome measures, as well as functional outcome using objective examination findings.

Hypothesis

The hypotheses of this study are that there will be improved graft healing and integration at three and nine months as measured by decreased signal intensity and heterogeneity on T2* MRI following administration of BMAC during ACL reconstruction compared to the control group. Additionally, patient reported outcomes and physical examination findings will be significantly improved at an earlier time in those who receive the BMAC treatment to their allograft compared to the control group.

Study Plan

This study will be a prospective randomized trial of patients undergoing arthroscopic reconstruction of full thickness anterior cruciate ligament (ACL) tears. All patients who sign consent and undergo full thickness ACL reconstruction will be enrolled in the study. Enrollment will continue until 32 patients, 16 in each group, are enrolled. Follow-up will take place at 6 weeks, 3 months, 6 months, 9 months, 12 months, and 24 months.

• Study Type: Interventional
• Enrollment (Estimated): 100
• Phase: Not Applicable

Contacts and Locations

Study Locations

• United States
  • Illinois
    • Chicago, Illinois, United States, 60521
      • Rush University Medical Center

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 18 years to 60 years (Adult)
• Accepts Healthy Volunteers: No

Description

Inclusion Criteria:

• English-speaking patients
• 18-60 years of age with acute ACL injury (<6 months from time of injury to reconstruction) and undergoing reconstruction with patellar tendon allograft
• Written informed consent is obtained

Exclusion Criteria:

• Revision Surgery
• Prior history of knee surgery
• Concomitant ligamentous injury
• Inability to obtain an MRI
• Inability to participate in follow-up for the study
• Any patient lacking decisional capability
• Diagnosed musculoskeletal cancer or any diagnosed cancer, other than
• musculoskeletal if not on long term remission (e.g. at least 5 years or negative biopsy at last exam), except basal cell carcinoma
• Patients who are at higher risk for post-surgical bleeding (e.g., bleeding disorder taking anticoagulants except low dose aspirin) or post-surgical infection (e.g., taking immunosuppressants; have a severe infection or a history of serious infection or use of systemic steroids)
• Known history of HIV, or has active Hepatitis B or active Hepatitis C
• Alcohol and drug (medication) abuse
• Pregnant or breast feeding women. Pregnancy as determined by a positive pregnancy test prior to procedure. Females of childbearing potential must agree to use an acceptable birth control method during study participation.
• Patients that have received PRP, other platelet-based product, or investigational treatment in another cell/biologic study for the target knee in the 12 months prior to the injection procedure
• Any clinically significant finding that would place the patient at health risk, impact the study, or affect the completion of the study
• Any psychiatric illness that would prevent comprehension of the details and nature of the study and interfere with follow-up clinic visits

Study Plan

How is the study designed?

Design Details

• Primary Purpose: Treatment
• Allocation: Randomized
• Interventional Model: Parallel Assignment
• Masking: Double

Number of Arms

2

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
Experimental: Mesenchymal Stem Cell Recipient; Patients in this group will receive between 1-4 ml of bone marrow aspirate concentrate (BMAC) containing mesenchymal stem cells (MSCs) obtained from their iliac crest. A small incision will be made on the anterior superior iliac spine in order to withdraw the aspirate. The aspirate will be ran through a centrifuge in order to isolate the BMAC containing MSCs. The BMAC will be injected into the ACL allograft prior to implanting into the patient.	Device: Autologous Bone Marrow Derived Mesenchymal Stem Cells; Autologous Bone Marrow Derived Mesenchymal Stem Cells injected into the ACL allograft prior to graft implantation.; Other Names: Sham Incision
Placebo Comparator: Control Sham Incision; Patients will receive a sham incision on the anterior superior iliac spine where the bone marrow aspirate is obtained in the the experimental group. This ensures proper blinding. The patient will receive the normal standard of care.	Other: Sham incision; Small incision over the site where bone marrow aspirate would have been obtained

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Signal to Noise Quotient	Signal intensity of the ACL graft on T2 weighted MRI imaging compared to a reference structures' signal intensity	9 months

Secondary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Side to side difference in anterior tibial translation	Anterior tibial translation measured by (Knee laxity Testing device) KT-1000 Arthrometer	6 weeks, 3 months, 6 months, 9 months, 1 year
Patient reported outcomes- Tegner Score	Patient reported outcome measure for patients receiving arthroscopic knee surgery	3 months, 6 months, 9 months, 12 months, 24 months
Patient reported outcomes- Knee Injury and Osteoarthritis Outcome Score for Joint Replacement (KOOS Jr)	Patient reported outcome measure for patients receiving arthroscopic knee surgery	3 months, 6 months, 9 months, 12 months, 24 months
Patient reported outcomes- International Knee Documentation Committee (IKDC) Score	Patient reported outcome measure for patients receiving arthroscopic knee surgery	3 months, 6 months, 9 months, 12 months, 24 months

Collaborators and Investigators

• Sponsor: Rush University Medical Center

Study record dates

Study Major Dates

• Study Start (Actual): November 3, 2017
• Primary Completion (Estimated): December 3, 2025
• Study Completion (Estimated): December 31, 2025

Study Registration Dates

• First Submitted: December 4, 2017
• First Submitted That Met QC Criteria: December 1, 2020
• First Posted (Actual): December 2, 2020

Study Record Updates

• Last Update Posted (Estimated): January 15, 2024
• Last Update Submitted That Met QC Criteria: January 11, 2024
• Last Verified: January 1, 2024

More Information

Terms related to this study

• Keywords: ACL; Anterior Cruciate Ligament; Mesenchymal stem cells; Stem Cell; augmentation; biologic
• Additional Relevant MeSH Terms: Wounds and Injuries; Leg Injuries; Knee Injuries; Rupture; Anterior Cruciate Ligament Injuries
• Other Study ID Numbers: 17082504

Plan for Individual participant data (IPD)

• Plan to Share Individual Participant Data (IPD)?: NO

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: No
• Studies a U.S. FDA-regulated device product: Yes
• product manufactured in and exported from the U.S.: No