The Effect of Exercise on Myokine Production in Aging Persons (EXI-AGE)

Does an Acute Exercise Bout Increases Soluble and Muscle Myokine Concentration in Healthy, Older Persons

The incidence of age-related diseases, such as cancer and cardiovascular diseases, rapidly rises. These days, age-related diseases cause the majority of all healthcare expenses. During ageing senescent cells secrete a range of harmful inflammatory signals, which leads to chronic inflammation (inflamm-aging) and accelerates aging throughout the rest of the body. Interestingly, people who regularly exercise show less signs of inflamm-aging compared to people with a sedentary lifestyle. Molecules secreted by skeletal muscles, called myokines, may play an important role. However, the underlying molecular mechanisms remain unknown. This pilot study aims to investigate if an acute bout of resistance training is an appropriate tool to induce an increase in the release of muscle derived myokines in aged persons.

Study Overview

• Status: Recruiting
• Conditions: Exercise; Aging
• Intervention / Treatment: Other: Exercise training; Behavioral: Questionnaire; Procedure: Percutaneous muscle biopsy; Procedure: Blood sample

• Study Type: Interventional
• Enrollment (Anticipated): 20
• Phase: Not Applicable

Contacts and Locations

• Study Contact: Name: Bert Op'T Eijnde, Prof. dr.; Phone Number: +3211292121; Email: bert.opteijnde@uhasselt.be
• Study Contact Backup: Name: Chiel Hex, MD.; Email: chiel.hex@uhasselt.be

Study Locations

• Belgium
  • Limburg
    • Diepenbeek, Limburg, Belgium, 3590
      • Recruiting
      • Hasselt University
      • Contact: Lena Fonteyn, Drs.; Phone Number: +3211269210; Email: lena.fonteyn@uhasselt.be
      • Contact: Leen Slaets, Dr.; Email: leen.slaets@uhasselt.be

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 65 years to 85 years (Older Adult)
• Accepts Healthy Volunteers: Yes
• Genders Eligible for Study: All

Description

Inclusion Criteria:

• Healthy persons

Exclusion Criteria:

• Smoking
• Infection < 2 weeks ago
• Received a vaccine < 2 weeks ago
• Allergy to Lidocain
• Immune deficiency
• Presence of a tumour or in treatment
• Usage of anti-coagulant medication

• Presence of following conditions:

  • Haemophilia
  • Auto-immune disease
  • Cardiovascular disease
  • Musculoskeletal diseases which refrains the patient from exercising

Study Plan

How is the study designed?

Design Details

• Primary Purpose: Basic Science
• Allocation: Randomized
• Interventional Model: Parallel Assignment
• Masking: Single

Number of Arms

2

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
Active Comparator: Aged individuals who perform exercise training; Aged individuals will perform an acute bout of resistance trainig (30 minutes). This training is focused on the lower limbs.	Other: Exercise training; Resistance training of the lower limbs; Behavioral: Questionnaire; Physical activity questionnaire; Procedure: Percutaneous muscle biopsy; Muscle biopsy of the vastus lateralis (m. quadriceps); Procedure: Blood sample; Venous blood sample
Sham Comparator: Aged individuals who stay sedentary; Aged individuals will remain seated for 30 minutes.	Behavioral: Questionnaire; Physical activity questionnaire; Procedure: Percutaneous muscle biopsy; Muscle biopsy of the vastus lateralis (m. quadriceps); Procedure: Blood sample; Venous blood sample

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Cytokine concentration	Measurement of multiple cytokines in blood (for example: IL-6, IL-15, IL-4, irisin...) with ELISA's or multiplex assays	Day 2
Myokine production	Immunohistochemistry staining (fluorescent) of multiple myokines (for example: IL-6, IL-15, IL-4, irisin..) and aging markers (p16,p21...) on muscle tissue	Day 2
Immune parameters	Flow cytometry panels of immune parameters in muscle tissue	Day 2
Myokine protein levels	Western blotting of multiple myokines in muscle tissue	Day 2
Myokine gene expression	Quantitative PCR (polymerase chain reaction) of markers in muscle tissue	Day 2

Secondary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Blood pressure	The force of circulating blood on the walls of the arteries. Measured using an electronic sphygmomanometer	Day 1
Resting Heart Rate	Measured using an electronic sphygmomanometer	Day 1
International Physical Activity Questionnaire (Short form)	Measures the subjective level of activity of the last seven days. 7 questions regarding amount of time spent on certain activities.	Day 1

Collaborators and Investigators

• Sponsor: Hasselt University
• Investigators: Principal Investigator: Chiel Hex, MD., University of Hasselt

Study record dates

Study Major Dates

• Study Start (Actual): September 15, 2022
• Primary Completion (Anticipated): October 15, 2023
• Study Completion (Anticipated): December 31, 2023

Study Registration Dates

• First Submitted: October 5, 2022
• First Submitted That Met QC Criteria: October 6, 2022
• First Posted (Actual): October 7, 2022

Study Record Updates

• Last Update Posted (Actual): March 31, 2023
• Last Update Submitted That Met QC Criteria: March 29, 2023
• Last Verified: March 1, 2023

More Information

Terms related to this study

• Other Study ID Numbers: B1152022000006

Plan for Individual participant data (IPD)

• Plan to Share Individual Participant Data (IPD)?: NO

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: No
• Studies a U.S. FDA-regulated device product: No