the Efficacy and Safety of Diosmin on Non-diabetic Patients With Non-alcoholic Steatohepatitis.

Clinical Study Evaluating the Efficacy and Safety of Diosmin on Non-diabetic Patients With Non-alcoholic Steatohepatitis.

This is a randomized, controlled study evaluating diosmin tablets administered daily for 3 months. The purpose of the study is to evaluate the efficacy and safety of diosmin on non-diabetic patients with non-alcoholic steatohepatitis.

Study Overview

• Status: Recruiting
• Conditions: Non Alcoholic Steatohepatitis
• Intervention / Treatment: Drug: Placebo; Drug: Diosmin

Detailed Description

The aim of the study is to test the implication of diosmin in fatty liver disease through evaluation of its efficacy and safety in non-diabetic patients with nonalcoholic steatohepatitis (NASH).

Patients and Methods:

This study will be a randomized double-blind placebo-controlled parallel study that will involve 48 non-diabetic patients with confirmed diagnosis of NASH. The patients will be recruited from outpatient Clinic of the internal medicine department, Tanta University Hospital, Tanta, Egypt. The diagnosis of NASH will be confirmed by imaging technique (increased liver echogenicity, stronger echoes in the hepatic parenchyma, vessel blurring, and narrowing of the lumen of the hepatic veins), mild to moderate elevation in aminotransferase activities (>2 but <5 times upper limit of normal), hepatic steatosis index (HIS) >36, HAIR score (hypertension, alanine aminotransferase level, insulin resistance) of 2 or 3 and cytokeratin-18 level >240 IU/L. The patients will be randomized in a 1:1 ratio by a neutral researcher using sealed envelopes methods with assignment codes for each available allocation to receive either diosmin 600 mg twice daily (Diosmin group; n = 24) or placebo twice daily (Placebo group; n = 24). The study duration will be 12 weeks.

• All participants and will be informed about the benefits and risks of the study. The expected risks that will be clarified to patients include diosmin related adverse effect; stomach pain, diarrhea, headache, skin redness, muscle pain, and altered heart rate.
• Any unexpected risks that will appear during the course of the research will be clarified to the participants and to the ethical committee on time. The data of the enrolled patients will be confidential.
• All enrolled patients will give their written informed consents. The study will be carried out between june2023 and june2025.Demography and anthropometric measurements At baseline and after intervention, all participants will be submitted to medical history taking, demography (age, sex, and medication history), physical examination with heart rate evaluation, measurement of weight and height with subsequent calculation of body mass index (BMI)=[Weight (kg)÷ Height2 (m)]. 2. Ultrasonographic examination Ultrasonography of the liver will be carried out at baseline and at the end of the study by the same operator. Ultrasonography Fatty Liver Indicator (US-FLI) is a scoring system ranging 2-8 based on the intensity of liver/kidney contrast, posterior attenuation of ultrasound beam, vessel blurring, difficult visualization of gallbladder wall, and difficult visualization of the diaphragm and areas of focal sparing. NAFLD is diagnosed by the minimum score ≥2. 3. Blood samples collection and biochemical measurements Before and after the intervention, 8 ml of venous blood will be withdrawn by antecubital venipuncture from each participant after overnight fasting (12 h fasting period) between 8:30 and 10:30 a.m. into plain test tubes. Three ml of the blood samples will be used for the assessment of complete blood count. The remaining 5 ml of blood will be centrifuged at 3000 rpm for 10 min. The separated serum will be divided into two portions. The first portion will be used for immediate determination of liver panel, fasting lipid profile and fasting blood glucose concentration. The second portion of the serum will be frozen at-80°C until analysis of the remaining parameters including malondialdehyde (MDA), tumor necrosis factor- alpha (TNF-α), fasting insulin and transforming growth factor-beta1 (TGF-β1).
• Complete blood count (CBC) will be determined by automated hematology analyzer.
• Liver enzymes (AST, ALT, and GGT) will be determined by kinetic method.
• Serum albumin level which will be assayed by colorimetric bromocresol green method or other available method.
• Fasting blood glucose will be determined by glucose oxidase method or glycated hemoglobin (HbA1c%) will be measured by commercially available method
• Lipid panel including total cholesterol (TC), triglyceride (TG) and HDLC will be assessed by enzymatic colorimetric method.
• Fasting insulin level will be determined by ELISA.
• Malondialdehyde (MDA) which will be assessed by colorimetric method.
• Tumor necrosis factor- alpha (TNF-α) will be determined by ELISA.
• Serum transforming growth factor-beta1 (TGF-β1) will be determined by ELISA.
• In addition, prothrombin time (PT) or INR will be assessed regularly at baseline and on monthly basis. 4. Calculated parameters
• Low density lipoprotein cholesterol (LDL-C) will be calculated using the

Friedewald formula17 as follows:

• [LDL-C=TC- HDL-C- (TG÷5)] provided that TG level is less than 400 mg/dl.

  • Very low density lipoprotein cholesterol will be calculated using the Friedewald formula17 as follows: o VLDL = TG/5.
  • The Homeostasis Model Assessment-insulin resistance (HOMA-IR) 18 will be calculated as follow: o HOMA-IR= (Fasting glucose × fasting insulin/405) when glucose is expressed by mg/dl and insulin is expressed by μIU/ml.
  • Hepatic steatosis index "HSI".
• Hepatic steatosis index includes gender, history of type 2 diabetes mellitus (T2DM), BMI, alanine transaminase "ALT", and aspartate transaminase "AST".

• HSI is calculated by the following formula:

  8 × (ALT/AST ratio) + BMI + 2 if female and + 2 if diabetic (0 since the study will be conducted on non-diabetic).

• HAIR score "hypertension, alanine aminotransferase level, insulin resistance". It is calculated from hypertension ≥140/90, ALT >40 and HOMA-IR >5.
• Presence of one item indicates HAIR score=1. o Presence of two item indicates HAIR score=2.

• Prescience of the 3 items indicates HAIR score=3.

  - Fibrosis risk scores: o Fibrosis index based on the 4 factors (FIB-4). FIB-4= Age (years) × AST (IU/l)/[platelet count (109

  /L) ×√ ALT (IU/l)].

• Aspartate transaminase-to-platelet ratio index (APRI).22 APRI =AST (IU/l)/(upper limit of normal) X 100 /platelet count (109

  /L).

• NAFLD fibrosis score (NFS) which depends on 7 parameters including age, BMI, diabetes, AST, ALT, platelets count, and albumin concentration.

  • Score <-1.455 predicts absence of significant fibrosis (F0-F2).
  • Score ≤ - 1.455- ≤ 0.675 predicts intermediate score
  • Score > 0.675 predicts presence of significant fibrosis (F3-F4).

• Study Type: Interventional
• Enrollment (Estimated): 48
• Phase: Phase 3

Contacts and Locations

• Study Contact: Name: merna tarek elsaeed; Phone Number: 00201551813013; Email: sgama676@gmail.com
• Study Contact Backup: Name: amira bahaa ismail; Phone Number: 00201142760505; Email: amira7794.aa@gmail.com

Study Locations

• Egypt
  • Tanta, Egypt
    • Recruiting
    • Tanta University
    • Contact: tanta university; Phone Number: 0020403317928; Email: tanta_unv@unv.tanta.edu.eg

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: Adult; Older Adult
• Accepts Healthy Volunteers: Yes

Description

Inclusion Criteria:

• Non-diabetic patients with or without hypertension.
• Both males and females.
• Age >18 years old.
• Overweight and obese patient: Body mass index (BMI) ≥ 25 kg/m2 <.40 kg/m2
• Patients with established diagnosis of NASH based on liver ultrasonography, mild to moderate elevation in aminotransferase activities (>2 but <5 times upper limit of normal), hepatic steatosis index (HIS) >36, HAIR score of 2 or 3.

Exclusion Criteria:

• Patients with BMI ≥ 40 kg/m2
• Patients with type 2 diabetes mellitus (T2DM) on the basis of a fasting plasma glucose (FPG) level ≥ 126 mg/dl (7mmol/L) or glycated hemoglobin (HbA1c) > 6.5% (48 mmol/mol).
• Alcohol consumption greater than 20 g per day for women or greater than 30 g for men for at least three consecutive months over the past 5 years.
• History of viral hepatitis, hemochromatosis, Wilson's disease, autoimmune hepatitis, primary biliary cirrhosis, sclerosing cholangitis, biliary obstruction, alpha-1 antitrypsin deficiency.
• Patients on medications interfere with lipid and carbohydrate metabolism (statin, fibrate, beta blockers, thiazide, corticosteroids, etc).
• Patients with cancer or with a history of cancer.
• Patients with thyroid disorder.
• Patients on medications associated with steatosis such as NSAIDs, amiodarone, tamoxifen, estrogen, sodium valproate, corticosteroids, and methotrexate.
• Patients with inflammatory diseases (rheumatoid arthritis, ulcerative colitis, etc).
• Patients on supplements known to have antioxidant activity such as vitamin E, vitamin C, zinc, and selenium.
• Patient with a history of cardiovascular diseases.
• Patients with arrhythmia or altered heart rate.
• Patients on blood thinning agents (warfarin, clopidogril, aspirin, etc), anticonvulsants (carbamazepine and phenytoin), muscle relaxants (chlorzoxazone) and non-steroidal anti-inflammatory drugs (diclofenac( in order to avoid potential pharmacodynamics and pharmacokinetic drug interactions with diosmin.
• Pregnant and breastfeeding women.
• Females on oral contraceptive pills will be also excluded.

Study Plan

How is the study designed?

Design Details

• Primary Purpose: Treatment
• Allocation: Randomized
• Interventional Model: Parallel Assignment
• Masking: Double

Number of Arms

2

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
Experimental: diosmin group; (Diosmin group; n = 24):will receive 600 mg twice daily	Drug: Diosmin; diosmin 600mg twice daily
Placebo Comparator: placebo group; placebo group n=24 :will receive placebo twice daily	Drug: Placebo; twice daily

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
the change in ultrasound including NAFLD fibrosis scor	The change in ultrasound through decreasing liver echogenicity, Lower echoes in the hepatic parenchyma, no vessel blurring, and decrease narrowing of the lumen of the hepatic veins Ultrasonography Fatty Liver Indicator (US-FLI) <2	at baseline then after 3 months
The change in fibrosis risk score	Score <-1.455 predicts absence of significant fibrosis (F0-F2)	at baseline then after 3 months

Secondary Outcome Measures

Outcome Measure	Measure Description	Time Frame
The secondary outcome is the change in biological biomarkers and other measured parameters	The change in liver panel	at baseline then after 3 months
secondary outcome is the change in biological biomarkers and other measured parameters	The change in the Homeostasis Model Assessment-insulin resistance (HOMA-IR)	at baseline then after 3 months
secondary outcome is the change in biological biomarkers and other measured parameters	The change in biological parameters (MDA, TNF-α, and TGF-β1)	at baseline then after 3 months

Collaborators and Investigators

• Sponsor: Tanta University
• Investigators: Principal Investigator: tarek mohamed mostafa, professor, Tanta University

Study record dates

Study Major Dates

• Study Start (Estimated): July 10, 2023
• Primary Completion (Estimated): October 10, 2023
• Study Completion (Estimated): June 10, 2025

Study Registration Dates

• First Submitted: June 21, 2023
• First Submitted That Met QC Criteria: July 5, 2023
• First Posted (Actual): July 12, 2023

Study Record Updates

• Last Update Posted (Actual): July 12, 2023
• Last Update Submitted That Met QC Criteria: July 5, 2023
• Last Verified: July 1, 2023

More Information

Terms related to this study

• Additional Relevant MeSH Terms: Digestive System Diseases; Liver Diseases; Fatty Liver; Non-alcoholic Fatty Liver Disease
• Other Study ID Numbers: 3626MS202/6/23

Plan for Individual participant data (IPD)

• Plan to Share Individual Participant Data (IPD)?: NO

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: No
• Studies a U.S. FDA-regulated device product: No