Micro RNA Genetic Signature in NSCLC Egyptian Patients
Micro RNA Genetic Signature in Non-small Cell Lung Cancer Egyptian Patients
This study will be carried out on 40 subjects at Chest department Tanta university hospital
The subjects will be classified into three groups:
- Group I: will include ten non smoker volunteers (control group I).
- GroupII: will include ten smoker volunteers (control groupII).
- Group III: will include twenty NSCLC patients confirmed by pathological examination of bronchoalveolar examination (BAL), brush and/or biopsy.
調査の概要
状態
条件
詳細な説明
The patients and control groups will be subjected to the following:
- Complete history taking and complete general examination.
- Complete chest examination.
- Radiological assessment including chest x ray (posteroanterior and lateral view).
- Computed tomography (CT) chest for all patients in group III and when needed in group I and II.
Laboratory investigations:
- Complete blood picture
- Fasting and post parandial blood sugar level
- liver function tests
- kidney function tests (urea and creatinine).
- Prothrombine time and activity.
- Zeil-Neelsen staining of sputum.
- Tumour markers including α Fetoprotein, Carcino Embryonic antigen (CEA), CA19-9, CA 125, CA15-3 and others.
- Venous blood samples will be collected from peripheral blood (5 ml) under complete aseptic technique. Serum samples will be obtained by centrifugation. Isolation of total RNA from serum pools will be performed. RNA profiles and quantification will be assessed using Microarray.
- Lung biopsy either transbronchial via bronchoscopy or peripherally via ultrasound , CT guided, thoracoscopy or open lung biopsy will be performed with isolation of total RNA from lung tissue biopsy. RNA profiles and quantification will be assessed using Microarray.
- Bronchoalveolar lavage will be performed with isolation of total RNA from it. RNA profiles will be assessed using Microarray.
Ethical considerations:
Informed consent will be taken from all subject's and include:
A. The aim of research. B. All data are confidential. C. All data will be used in research only.
For patient's privacy:
A. There will be a code number for each patient in special folder. B. The results of research will be used in scientific publishing only.
- Unexpected risks that appear during the courses of research will be cleared to the participants and the ethical committee on time.
研究の種類
入学 (実際)
連絡先と場所
研究場所
-
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Gharbia
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Tanta、Gharbia、エジプト、31111
- Chest Department, Faculty of Medicine, Tanta University
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参加基準
適格基準
就学可能な年齢
健康ボランティアの受け入れ
受講資格のある性別
サンプリング方法
調査対象母集団
This study will be carried out on 40 subjects at Chest department Tanta university hospital.
The subjects will be classified into three groups:
- Group I: will include ten non smoker volunteers (control group I).
- GroupII: will include ten smoker volunteers (control groupII).
- Group III: will include twenty NSCLC patients confirmed by pathological examination of bronchoalveolar examination (BAL), brush and/or biopsy.
説明
Inclusion Criteria:
- Inclusion criteria for patients:
Non small cell lung cancer patients recently diagnosed who did not receive chemotherapy, surgery or radiation therapy.
-
Exclusion Criteria:
- Exclusion criteria for patients:
- Diagnosis of asthma or COPD.
- Broncheiactasis.
- upper/lower respiratory tract infection in the preceding 4 weeks.
- Active pulmonary tuberculosis.
- Associated cancer beside lung cancer.
- Patients who received chemotherapy, surgery or radiation therapy previous to the sample collection.
Exclusion criteria for control subjects:
- Diagnosis of asthma or COPD.
- Broncheiactasis.
- Upper/lower respiratory tract infection in the preceding 4 weeks.
- Active pulmonary tuberculosis
- Smoking in group I.
研究計画
研究はどのように設計されていますか?
デザインの詳細
コホートと介入
グループ/コホート |
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Control group 1
ten non smoker volunteers
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Control group 2
ten smoker volunteers
|
NSCLC patients
twenty NSCLC patients confirmed by pathological examination of bronchoalveolar examination (BAL), brush and/or biopsy
|
この研究は何を測定していますか?
主要な結果の測定
結果測定 |
時間枠 |
---|---|
Detect micro RNA genetic signature pattern in non-small cell lung cancer Egyptian patients assessed using Microarray
時間枠:one year
|
one year
|
協力者と研究者
スポンサー
捜査官
- 主任研究者:Abdel-Aziz A Zidan, PHD、Director of Genomics and Proteomics Unit, Center of Excellence in Cancer Research, Tanta University
- スタディチェア:Amgad A Farahat, MD、Chest Department, Faculty of Medicine, Tanta University
- 主任研究者:Ayman H Abd El-Zaher, MD、Chest Department, Faculty of Medicine, Tanta University
- 主任研究者:Said M Hammad, MD、Clinical Pathology Department, Faculty of Medicine, Tanta University
- 主任研究者:Adel S Bediwy, MD、Chest Department, Faculty of Medicine, Tanta University
- 主任研究者:Ayman A Al Saka, MD、Pathology Department, Faculty of Medicine, Tanta University
- 主任研究者:Samar A Mansour, Master、Chest Department, Faculty of Medicine, Tanta University
出版物と役立つリンク
一般刊行物
- Tang D, Shen Y, Wang M, Yang R, Wang Z, Sui A, Jiao W, Wang Y. Identification of plasma microRNAs as novel noninvasive biomarkers for early detection of lung cancer. Eur J Cancer Prev. 2013 Nov;22(6):540-8. doi: 10.1097/CEJ.0b013e32835f3be9.
- Keller A, Leidinger P, Borries A, Wendschlag A, Wucherpfennig F, Scheffler M, Huwer H, Lenhof HP, Meese E. miRNAs in lung cancer - studying complex fingerprints in patient's blood cells by microarray experiments. BMC Cancer. 2009 Oct 6;9:353. doi: 10.1186/1471-2407-9-353.
- Leidinger P, Keller A, Meese E. MicroRNAs - Important Molecules in Lung Cancer Research. Front Genet. 2012 Jan 23;2:104. doi: 10.3389/fgene.2011.00104. eCollection 2011.
研究記録日
主要日程の研究
研究開始
一次修了 (実際)
研究の完了 (実際)
試験登録日
最初に提出
QC基準を満たした最初の提出物
最初の投稿 (見積もり)
学習記録の更新
投稿された最後の更新 (見積もり)
QC基準を満たした最後の更新が送信されました
最終確認日
詳しくは
この情報は、Web サイト clinicaltrials.gov から変更なしで直接取得したものです。研究の詳細を変更、削除、または更新するリクエストがある場合は、register@clinicaltrials.gov。 までご連絡ください。 clinicaltrials.gov に変更が加えられるとすぐに、ウェブサイトでも自動的に更新されます。
非小細胞肺がんの臨床試験
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