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Micro RNA Genetic Signature in NSCLC Egyptian Patients
Micro RNA Genetic Signature in Non-small Cell Lung Cancer Egyptian Patients
This study will be carried out on 40 subjects at Chest department Tanta university hospital
The subjects will be classified into three groups:
- Group I: will include ten non smoker volunteers (control group I).
- GroupII: will include ten smoker volunteers (control groupII).
- Group III: will include twenty NSCLC patients confirmed by pathological examination of bronchoalveolar examination (BAL), brush and/or biopsy.
Studie Overzicht
Toestand
Conditie
Gedetailleerde beschrijving
The patients and control groups will be subjected to the following:
- Complete history taking and complete general examination.
- Complete chest examination.
- Radiological assessment including chest x ray (posteroanterior and lateral view).
- Computed tomography (CT) chest for all patients in group III and when needed in group I and II.
Laboratory investigations:
- Complete blood picture
- Fasting and post parandial blood sugar level
- liver function tests
- kidney function tests (urea and creatinine).
- Prothrombine time and activity.
- Zeil-Neelsen staining of sputum.
- Tumour markers including α Fetoprotein, Carcino Embryonic antigen (CEA), CA19-9, CA 125, CA15-3 and others.
- Venous blood samples will be collected from peripheral blood (5 ml) under complete aseptic technique. Serum samples will be obtained by centrifugation. Isolation of total RNA from serum pools will be performed. RNA profiles and quantification will be assessed using Microarray.
- Lung biopsy either transbronchial via bronchoscopy or peripherally via ultrasound , CT guided, thoracoscopy or open lung biopsy will be performed with isolation of total RNA from lung tissue biopsy. RNA profiles and quantification will be assessed using Microarray.
- Bronchoalveolar lavage will be performed with isolation of total RNA from it. RNA profiles will be assessed using Microarray.
Ethical considerations:
Informed consent will be taken from all subject's and include:
A. The aim of research. B. All data are confidential. C. All data will be used in research only.
For patient's privacy:
A. There will be a code number for each patient in special folder. B. The results of research will be used in scientific publishing only.
- Unexpected risks that appear during the courses of research will be cleared to the participants and the ethical committee on time.
Studietype
Inschrijving (Werkelijk)
Contacten en locaties
Studie Locaties
-
-
Gharbia
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Tanta, Gharbia, Egypte, 31111
- Chest Department, Faculty of Medicine, Tanta University
-
-
Deelname Criteria
Geschiktheidscriteria
Leeftijden die in aanmerking komen voor studie
Accepteert gezonde vrijwilligers
Geslachten die in aanmerking komen voor studie
Bemonsteringsmethode
Studie Bevolking
This study will be carried out on 40 subjects at Chest department Tanta university hospital.
The subjects will be classified into three groups:
- Group I: will include ten non smoker volunteers (control group I).
- GroupII: will include ten smoker volunteers (control groupII).
- Group III: will include twenty NSCLC patients confirmed by pathological examination of bronchoalveolar examination (BAL), brush and/or biopsy.
Beschrijving
Inclusion Criteria:
- Inclusion criteria for patients:
Non small cell lung cancer patients recently diagnosed who did not receive chemotherapy, surgery or radiation therapy.
-
Exclusion Criteria:
- Exclusion criteria for patients:
- Diagnosis of asthma or COPD.
- Broncheiactasis.
- upper/lower respiratory tract infection in the preceding 4 weeks.
- Active pulmonary tuberculosis.
- Associated cancer beside lung cancer.
- Patients who received chemotherapy, surgery or radiation therapy previous to the sample collection.
Exclusion criteria for control subjects:
- Diagnosis of asthma or COPD.
- Broncheiactasis.
- Upper/lower respiratory tract infection in the preceding 4 weeks.
- Active pulmonary tuberculosis
- Smoking in group I.
Studie plan
Hoe is de studie opgezet?
Ontwerpdetails
Cohorten en interventies
Groep / Cohort |
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Control group 1
ten non smoker volunteers
|
Control group 2
ten smoker volunteers
|
NSCLC patients
twenty NSCLC patients confirmed by pathological examination of bronchoalveolar examination (BAL), brush and/or biopsy
|
Wat meet het onderzoek?
Primaire uitkomstmaten
Uitkomstmaat |
Tijdsspanne |
---|---|
Detect micro RNA genetic signature pattern in non-small cell lung cancer Egyptian patients assessed using Microarray
Tijdsspanne: one year
|
one year
|
Medewerkers en onderzoekers
Sponsor
Onderzoekers
- Hoofdonderzoeker: Abdel-Aziz A Zidan, PHD, Director of Genomics and Proteomics Unit, Center of Excellence in Cancer Research, Tanta University
- Studie stoel: Amgad A Farahat, MD, Chest Department, Faculty of Medicine, Tanta University
- Hoofdonderzoeker: Ayman H Abd El-Zaher, MD, Chest Department, Faculty of Medicine, Tanta University
- Hoofdonderzoeker: Said M Hammad, MD, Clinical Pathology Department, Faculty of Medicine, Tanta University
- Hoofdonderzoeker: Adel S Bediwy, MD, Chest Department, Faculty of Medicine, Tanta University
- Hoofdonderzoeker: Ayman A Al Saka, MD, Pathology Department, Faculty of Medicine, Tanta University
- Hoofdonderzoeker: Samar A Mansour, Master, Chest Department, Faculty of Medicine, Tanta University
Publicaties en nuttige links
Algemene publicaties
- Tang D, Shen Y, Wang M, Yang R, Wang Z, Sui A, Jiao W, Wang Y. Identification of plasma microRNAs as novel noninvasive biomarkers for early detection of lung cancer. Eur J Cancer Prev. 2013 Nov;22(6):540-8. doi: 10.1097/CEJ.0b013e32835f3be9.
- Keller A, Leidinger P, Borries A, Wendschlag A, Wucherpfennig F, Scheffler M, Huwer H, Lenhof HP, Meese E. miRNAs in lung cancer - studying complex fingerprints in patient's blood cells by microarray experiments. BMC Cancer. 2009 Oct 6;9:353. doi: 10.1186/1471-2407-9-353.
- Leidinger P, Keller A, Meese E. MicroRNAs - Important Molecules in Lung Cancer Research. Front Genet. 2012 Jan 23;2:104. doi: 10.3389/fgene.2011.00104. eCollection 2011.
Studie record data
Bestudeer belangrijke data
Studie start
Primaire voltooiing (Werkelijk)
Studie voltooiing (Werkelijk)
Studieregistratiedata
Eerst ingediend
Eerst ingediend dat voldeed aan de QC-criteria
Eerst geplaatst (Schatting)
Updates van studierecords
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Laatste update ingediend die voldeed aan QC-criteria
Laatst geverifieerd
Meer informatie
Termen gerelateerd aan deze studie
Aanvullende relevante MeSH-voorwaarden
Andere studie-ID-nummers
- 2858/11/14
Deze informatie is zonder wijzigingen rechtstreeks van de website clinicaltrials.gov gehaald. Als u verzoeken heeft om uw onderzoeksgegevens te wijzigen, te verwijderen of bij te werken, neem dan contact op met register@clinicaltrials.gov. Zodra er een wijziging wordt doorgevoerd op clinicaltrials.gov, wordt deze ook automatisch bijgewerkt op onze website .
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