- ICH GCP
- 미국 임상 시험 레지스트리
- 임상시험 NCT03008759
Evaluation in Vivo of Dentifrice With Nanoencapsulated Fluoride
Evaluation in Vivo of Experimental Dentifrice With Nanoencapsulated Fluoride
연구 개요
상태
정황
상세 설명
Study design It is a quantitative study of double-blind randomized crossover clinical type with inductive approach and documentation of technical and direct observation with measurements in the laboratory.
In order to assess the bioavailability of intraoral fluoride week after the use of fluoride toothpastes containing Nanoencapsulated Fluoride, it will be collected biofilm samples 1h and 12 h after brushing; and unstimulated saliva in times of 3, 15, 30, 45, 60 minutes and 12 hours after. The data will be obtained by physical and chemical analysis of the samples of fluoride (F). Therefore, the study will consist of two interrelated steps: a clinical phase for biofilm collection and saliva; and laboratory stage for fluoride analysis in the samples.
Will be conducted pilot test with three participants before the start of the study.
- Sample Size The sample size calculation was performed using fluoride concentration data in dental biofilm previous study and taking effect size difference between the experimental dentifrice (100% Nanoencapsulated Fluoride ) and positive control of at least 3.4 ppm of F- in biofilm after 12 hours, namely, 25%. Admitting experimental protocol with beta error of 10% alpha error of 5%, one-tailed and statistical power of 90%, the sample size was calculated for 6 people. For the calculation we used the Excel software and mathematical formula for clinical trials of superiority given. Adding sample loss resulted in 12 people.
Experimental Dentifrice For this study will be used four toothpastes: 50% Nanoencapsulated Fluoride, 100% Nanoencapsulated Fluoride, Free sodium fluoride, Without fluoride. The toothpaste, made available by the company from Brazil are packed in the same packaging and have the taste of mint. Except wash out the tubes with toothpaste will be identified by letters (A, B and C) by an independent researcher. The letters code will not be revealed to the participants nor the researcher who will accompany them. These will be drawn at random for daily use for seven days in four times, giving an interval of one week wash-out between them.
In these periods of wash-out will use a toothpaste without fluoride. In the end, all study participants will use the 5 experimental dentifrice at different times.
Clinical phase The participant will be informed about the study and after its acceptance, he/she will initiate the study. Initially will be clinical examination in order to report the presence of any of the exclusion criteria already listed. After verifying the absence of these criteria, there will be a dental prophylaxis pumice for removal of this biofilm and in some cases may be made supragingival scraping. The patient will be advised not to make use of any fluoride product during the experiment. At first, volunteers are instructed to use a toothpaste without fluoride (wash out) the first week, in order to equate the study participants and eliminate any potential carryover effects of toothpaste used previously.
The participant will get a tube of toothpaste and will be instructed to brush their teeth for 1 minute and rinse with 10 mL of water twice a day (morning and night). On the seventh day, the participant will be instructed to brush only the occlusal surfaces of your teeth, to allow biofilm accumulation of flat surfaces. When going to bed, you are warned not to eat or drink (except water) as well as not brushing your teeth until the next day. On the following morning, approximately 12 hours after the last brushing and fasting, the first dental biofilm sample and unstimulated saliva is collected. It will be used to remove biofilm Hollenback vestibular faces, interproximal, palatal and lingual right hemiarch and deposited in a pre-weighed Eppendorf tube in an analytical balance. To saliva collection, the participant, at rest, will be instructed to spit for a minute in a graduated glass tube. After that, the participant will brush the occlusal surfaces for 1 min and rinse with 10 mL of water. Soon after, will new collection of saliva and plaque. Saliva samples will give up in times of 3, 15, 30, 45 and 60 minutes after brushing, while the biofilm left hemiarch will be collected one hour after brushing. After the collection will be offered at breakfast organized by researchers participating in the crown of that research center.
- Laboratorial phase Samples will be evaluated through physical-chemical analysis of fluoride using calibrated fluoride electrode. Samples will be analyzed for fluoride concentration using ion-specific electrode after diffusion facilitated with hexamethyl disiloxane (HMDS). The calibration curve is made for each analysis. All analyzes will be done in triplicate and be submitted for validation.
- Data analysis The study data will be analyzed by inferential statistics considering p <0.05.
연구 유형
등록 (예상)
단계
- 2 단계
- 1단계
연락처 및 위치
연구 장소
-
-
Paraíba
-
Joao Pessoa, Paraíba, 브라질
- Federal University of Paraiba
-
-
참여기준
자격 기준
공부할 수 있는 나이
건강한 자원 봉사자를 받아들입니다
연구 대상 성별
설명
Inclusion Criteria:
- resident in João Pessoa
- Decayed, Missing, Filled Index<6
- Healthy
Exclusion Criteria:
- orthodontic treatment
- gingivitis
- Periodontal disease
- Smokers
- Present active tooth decay
- Tooth sensitivity
- Those undergoing treatment with fluoride products and / or use of antimicrobials in the past four weeks
공부 계획
연구는 어떻게 설계됩니까?
디자인 세부사항
- 주 목적: 방지
- 할당: 무작위
- 중재 모델: 크로스오버 할당
- 마스킹: 삼루타
무기와 개입
참가자 그룹 / 팔 |
개입 / 치료 |
---|---|
위약 비교기: Placebo
Dentifrice without fluoride
|
Use of few of dentifrice 100% Nanoencapsulated Fluoride on brushing for one week, twice day.
Use of few of dentifrice 50% Nanoencapsulated Fluoride on brushing for one week, twice day.
Use of few of dentifrice on brushing for one week, twice day.
It's for the positive control of fluorid
|
활성 비교기: Dentifrice standard
Dentifrice 1100 ppm sodium fluoride (NaF).
Positive control
|
Use of few of dentifrice on brushing for one week, twice day.
It's for the positive control of fluorid
|
실험적: Dentifrice 50% Nano-F
Dentifrice experimental with Fluoride being half of fluoride Nanoencapsulated and other half freeform of NaF
|
Use of few of dentifrice 50% Nanoencapsulated Fluoride on brushing for one week, twice day.
|
실험적: Dentifrice 100% Nano-F
Dentifrice experimental with fluoride 100% Nanoencapsulated
|
Use of few of dentifrice 100% Nanoencapsulated Fluoride on brushing for one week, twice day.
|
연구는 무엇을 측정합니까?
주요 결과 측정
결과 측정 |
측정값 설명 |
기간 |
---|---|---|
Bioavailability of intraoral fluoride (biofilm and saliva)
기간: six months
|
collection of dental plaque(biofilm) and saliva
|
six months
|
공동 작업자 및 조사자
수사관
- 연구 책임자: Fábio Sampaio, Doctor, University Federal of Paraiba
간행물 및 유용한 링크
일반 간행물
- Buzalaf MA, Vilhena FV, Iano FG, Grizzo L, Pessan JP, Sampaio FC, Oliveira RC. The effect of different fluoride concentrations and pH of dentifrices on plaque and nail fluoride levels in young children. Caries Res. 2009;43(2):142-6. doi: 10.1159/000211717. Epub 2009 Apr 8.
- Whitford GM, Wasdin JL, Schafer TE, Adair SM. Plaque fluoride concentrations are dependent on plaque calcium concentrations. Caries Res. 2002 Jul-Aug;36(4):256-65. doi: 10.1159/000063931.
- Pessan JP, Silva SM, Lauris JR, Sampaio FC, Whitford GM, Buzalaf MA. Fluoride uptake by plaque from water and from dentifrice. J Dent Res. 2008 May;87(5):461-5. doi: 10.1177/154405910808700501.
- Pessan JP, Pinto Alves KM, Italiani Fde M, Ramires I, Lauris JR, Whitford GM, Toumba KJ, Robinson C, Buzalaf MA. Distribution of fluoride and calcium in plaque biofilms after the use of conventional and low-fluoride dentifrices. Int J Paediatr Dent. 2014 Jul;24(4):293-302. doi: 10.1111/ipd.12073.
- Pessan JP, Conceicao JM, Grizzo LT, Szekely M, Fazakas Z, Buzalaf MA. Intraoral fluoride levels after use of conventional and high-fluoride dentifrices. Clin Oral Investig. 2015 May;19(4):955-8. doi: 10.1007/s00784-015-1426-3. Epub 2015 Feb 14.
- Duckworth RM, Jones S. On the relationship between the rate of salivary flow and salivary fluoride clearance. Caries Res. 2015;49(2):141-6. doi: 10.1159/000365949. Epub 2015 Jan 27.
- Duckworth RM, Morgan SN, Gilbert RJ. Oral fluoride measurements for estimation of the anti-caries efficacy of fluoride treatments. J Dent Res. 1992 Apr;71 Spec No:836-40. doi: 10.1177/002203459207100S09.
연구 기록 날짜
연구 주요 날짜
연구 시작
기본 완료 (실제)
연구 완료 (예상)
연구 등록 날짜
최초 제출
QC 기준을 충족하는 최초 제출
처음 게시됨 (추정)
연구 기록 업데이트
마지막 업데이트 게시됨 (추정)
QC 기준을 충족하는 마지막 업데이트 제출
마지막으로 확인됨
추가 정보
이 정보는 변경 없이 clinicaltrials.gov 웹사이트에서 직접 가져온 것입니다. 귀하의 연구 세부 정보를 변경, 제거 또는 업데이트하도록 요청하는 경우 register@clinicaltrials.gov. 문의하십시오. 변경 사항이 clinicaltrials.gov에 구현되는 즉시 저희 웹사이트에도 자동으로 업데이트됩니다. .
Dentifrice 100% Nano-F에 대한 임상 시험
-
International Centre for Diarrhoeal Disease Research...London School of Hygiene and Tropical Medicine완전한
-
Organon and Co완전한
-
Gilead Sciences모병HIV 감염 | 후천성 면역 결핍 증후군(AIDS)미국, 남아프리카, 아르헨티나, 파나마, 태국, 짐바브웨, 영국, 우간다
-
Institut de Recherche en Sciences de la Sante,...완전한