Mitochondrial Energy Metabolism in Obese Women
2019년 4월 27일 업데이트: Julio Sergio Marchini, University of Sao Paulo
Mitochondrial Energy Metabolism in Obese Women Undergoing Concurrent Physical Training
Considering that the failure of the treatment of obesity is justified by the multifactorial pathophysiology of this morbidity, the present project has the following hypotheses:
- The occurrence of obesity is due to the derange,ent of mitochondrial energy metabolism ;
- The unbalance is therapeutically modified through physical training ;
- Obesity courses with the break-down in energy metabolism mitochondrial disease associated with systemic inflammatory characteristics that can be corrected through a combined long-term physical training program.
This study have as objective : to analyse changes in mitochondrial function, inflammatory profile, oxidative stress and energy metabolism caused by concurrent physical training in obese women.
연구 개요
상세 설명
Specific objectives:
Body composition by deuterium oxide; Metabolic rate of resting and oxidation of substrates by indirect calorimetry; Proinflammatory cytokines Anti-inflammatory cytokines Oxidative Stress: Malondialdehyde, Superoxide Dismutase, Glutathione-Peroxidase; Fatty acids: ceramide and palmitate; Mitochondrial respiration and citrate synthase enzyme; Quantify and qualify: mitochondrial number, endoplasmic reticulum structure, adipose cell size; Gene expression, quantify by microscopy and analyze the protein by western blot.
The study began with 20 women, however, there was withdrawal of 6, ending with 14 women.
연구 유형
중재적
등록 (실제)
14
단계
- 해당 없음
연락처 및 위치
이 섹션에서는 연구를 수행하는 사람들의 연락처 정보와 이 연구가 수행되는 장소에 대한 정보를 제공합니다.
연구 장소
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SP
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Ribeirao Preto, SP, 브라질, 14.048-900
- Camila Fernanda Cunha Brandão
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참여기준
연구원은 적격성 기준이라는 특정 설명에 맞는 사람을 찾습니다. 이러한 기준의 몇 가지 예는 개인의 일반적인 건강 상태 또는 이전 치료입니다.
자격 기준
공부할 수 있는 나이
20년 (성인)
건강한 자원 봉사자를 받아들입니다
아니
연구 대상 성별
여성
설명
Inclusion Criteria:
- This study included women with obesity (BMI of 30 to 40 kg / m²), sedentary, with no associated comorbidity, convenience sample
Exclusion Criteria:
- Women who have undergone bariatric surgery, menopause, cancer or any metabolic disease, smokers, alcoholics, who are in use of drugs that act directly on the metabolism and that have medical impediment to the practice of physical exercise.
공부 계획
이 섹션에서는 연구 설계 방법과 연구가 측정하는 내용을 포함하여 연구 계획에 대한 세부 정보를 제공합니다.
연구는 어떻게 설계됩니까?
디자인 세부사항
- 주 목적: 치료
- 할당: 해당 없음
- 중재 모델: 단일 그룹 할당
- 마스킹: 없음(오픈 라벨)
무기와 개입
참가자 그룹 / 팔 |
개입 / 치료 |
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다른: Physical Training
There was concurrent physical training intervention: strength and aerobic exercises in the same session. Duration: 2 weeks of adaptation and learning to exercise, 8 weeks of physical training. Frequency: 3 times per week Duration: 55 minutes each session. Intensity: 75 to 90% of maximum heart rate. |
Intervention with concurrent physical training: strength and aerobic exercises in the same session. Duration: 2 weeks of adaptation to physical exercise, 8 weeks of training. Frequency: 3 times a week. Time: 55 minutes each session. Intensity: 75 to 90% of maximum heart rate. |
연구는 무엇을 측정합니까?
주요 결과 측정
결과 측정 |
측정값 설명 |
기간 |
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Changes Body weight
기간: Two times: (1) First day and (2) 10 weeks after adaptation and intervention
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Body weight was measured by digital balance before and after the intervention
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Two times: (1) First day and (2) 10 weeks after adaptation and intervention
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Changes Body composition
기간: Two times: (1) First day and (2) 10 weeks after adaptation and intervention
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The change in body composition through deuterium oxide was evaluated.
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Two times: (1) First day and (2) 10 weeks after adaptation and intervention
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Changes White adipose tissue biopsy
기간: Two times: (1) First day and (2) 10 weeks after adaptation and intervention
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A subcutaneous tissue sample was collected for analysis of: mitochondrial respiration, citrate synthase enzyme, gene expression (UCP1, 2 and 3).
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Two times: (1) First day and (2) 10 weeks after adaptation and intervention
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Changes Indirect calorimetry
기간: Two times: (1) First day and (2) 10 weeks after adaptation and intervention
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With a gas analyzer (indirect calorimeter), we evaluated the metabolic rate and rest (REE) and oxidation of substrates (Lipids and carbohydrates).
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Two times: (1) First day and (2) 10 weeks after adaptation and intervention
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Changes in fatty acids
기간: Two times: (1) First day and (2) 10 weeks after adaptation and intervention
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Collected in lithium heparin tubes, they were centrifuged.
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Two times: (1) First day and (2) 10 weeks after adaptation and intervention
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Changes oxidative stress
기간: Two times: (1) First day and (2) 10 weeks after adaptation and intervention
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Collected in lithium heparin tubes, they were centrifuged.
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Two times: (1) First day and (2) 10 weeks after adaptation and intervention
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Changes inflammatory cytokines
기간: Two times: (1) First day and (2) 10 weeks after adaptation and intervention
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Collected in lithium heparin tubes, they were centrifuged.
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Two times: (1) First day and (2) 10 weeks after adaptation and intervention
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Changes in total cholesterol
기간: Two times: (1) First day and (2) 10 weeks after adaptation and intervention
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Collected in lithium heparin tubes, they were centrifuged.
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Two times: (1) First day and (2) 10 weeks after adaptation and intervention
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Changes Physical Performance
기간: Two times: (1) First day and (2) 10 weeks after adaptation and intervention
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Based on the Shuttle Walking Test adaptation.
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Two times: (1) First day and (2) 10 weeks after adaptation and intervention
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Changes in Determination of Lactate
기간: Two times: (1) First day and (2) 10 weeks after adaptation and intervention
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Blood samples were collected by manual puncture of the earlobe in previously calibrated and heparinized capillary tubes, stored in eppendorf with sodium fluoride.
Analyzed by electrochemical lactate analyser.
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Two times: (1) First day and (2) 10 weeks after adaptation and intervention
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Changes Food intake
기간: Two times: (1) First day and (2) 10 weeks after adaptation and intervention
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Food registry of 3 days, the quantification of the daily intake of nutrients will still be made using software.
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Two times: (1) First day and (2) 10 weeks after adaptation and intervention
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Changes Nitrogen Balance
기간: Two times: (1) First day and (2) 10 weeks after adaptation and intervention
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Through the collection of urine of 24 hours the dosage of urinary nitrogen will be made by the chemiluminescence method for determination of protein nitrogen.
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Two times: (1) First day and (2) 10 weeks after adaptation and intervention
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Changes Telomere length
기간: Two times: (1) First day and (2) 10 weeks after adaptation and intervention
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peripheral blood in ethylenediaminetetraacetic acid tubes and genomic DNA was automatically extracted from Peripheral Blood Mononuclear Cell.
The relative quantification of Telomere length was determined using the telomere to single copy gene ratio by Quantitative Polymerase Chain Reaction (qPCR).
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Two times: (1) First day and (2) 10 weeks after adaptation and intervention
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공동 작업자 및 조사자
여기에서 이 연구와 관련된 사람과 조직을 찾을 수 있습니다.
간행물 및 유용한 링크
연구에 대한 정보 입력을 담당하는 사람이 자발적으로 이러한 간행물을 제공합니다. 이것은 연구와 관련된 모든 것에 관한 것일 수 있습니다.
일반 간행물
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연구 기록 날짜
이 날짜는 ClinicalTrials.gov에 대한 연구 기록 및 요약 결과 제출의 진행 상황을 추적합니다. 연구 기록 및 보고된 결과는 공개 웹사이트에 게시되기 전에 특정 품질 관리 기준을 충족하는지 확인하기 위해 국립 의학 도서관(NLM)에서 검토합니다.
연구 주요 날짜
연구 시작 (실제)
2016년 4월 1일
기본 완료 (실제)
2016년 7월 1일
연구 완료 (실제)
2016년 9월 15일
연구 등록 날짜
최초 제출
2017년 4월 10일
QC 기준을 충족하는 최초 제출
2017년 4월 13일
처음 게시됨 (실제)
2017년 4월 18일
연구 기록 업데이트
마지막 업데이트 게시됨 (실제)
2019년 4월 30일
QC 기준을 충족하는 마지막 업데이트 제출
2019년 4월 27일
마지막으로 확인됨
2019년 4월 1일
추가 정보
이 연구와 관련된 용어
추가 관련 MeSH 약관
기타 연구 ID 번호
- Process HCRP: 1.387.040/2016
개별 참가자 데이터(IPD) 계획
개별 참가자 데이터(IPD)를 공유할 계획입니까?
아니요
약물 및 장치 정보, 연구 문서
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아니
미국 FDA 규제 기기 제품 연구
아니
이 정보는 변경 없이 clinicaltrials.gov 웹사이트에서 직접 가져온 것입니다. 귀하의 연구 세부 정보를 변경, 제거 또는 업데이트하도록 요청하는 경우 register@clinicaltrials.gov. 문의하십시오. 변경 사항이 clinicaltrials.gov에 구현되는 즉시 저희 웹사이트에도 자동으로 업데이트됩니다. .
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