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Mitochondrial Energy Metabolism in Obese Women

27 april 2019 uppdaterad av: Julio Sergio Marchini, University of Sao Paulo

Mitochondrial Energy Metabolism in Obese Women Undergoing Concurrent Physical Training

Considering that the failure of the treatment of obesity is justified by the multifactorial pathophysiology of this morbidity, the present project has the following hypotheses:

  1. The occurrence of obesity is due to the derange,ent of mitochondrial energy metabolism ;
  2. The unbalance is therapeutically modified through physical training ;
  3. Obesity courses with the break-down in energy metabolism mitochondrial disease associated with systemic inflammatory characteristics that can be corrected through a combined long-term physical training program.

This study have as objective : to analyse changes in mitochondrial function, inflammatory profile, oxidative stress and energy metabolism caused by concurrent physical training in obese women.

Studieöversikt

Status

Avslutad

Betingelser

Intervention / Behandling

Detaljerad beskrivning

Specific objectives:

Body composition by deuterium oxide; Metabolic rate of resting and oxidation of substrates by indirect calorimetry; Proinflammatory cytokines Anti-inflammatory cytokines Oxidative Stress: Malondialdehyde, Superoxide Dismutase, Glutathione-Peroxidase; Fatty acids: ceramide and palmitate; Mitochondrial respiration and citrate synthase enzyme; Quantify and qualify: mitochondrial number, endoplasmic reticulum structure, adipose cell size; Gene expression, quantify by microscopy and analyze the protein by western blot.

The study began with 20 women, however, there was withdrawal of 6, ending with 14 women.

Studietyp

Interventionell

Inskrivning (Faktisk)

14

Fas

  • Inte tillämpbar

Kontakter och platser

Det här avsnittet innehåller kontaktuppgifter för dem som genomför studien och information om var denna studie genomförs.

Studieorter

  • Brasilien
    • SP
      • Ribeirao Preto, SP, Brasilien, 14.048-900
        • Camila Fernanda Cunha Brandão

Deltagandekriterier

Forskare letar efter personer som passar en viss beskrivning, så kallade behörighetskriterier. Några exempel på dessa kriterier är en persons allmänna hälsotillstånd eller tidigare behandlingar.

Urvalskriterier

Åldrar som är berättigade till studier

20 år till 40 år (Vuxen)

Tar emot friska volontärer

Nej

Kön som är behöriga för studier

Kvinna

Beskrivning

Inclusion Criteria:

  • This study included women with obesity (BMI of 30 to 40 kg / m²), sedentary, with no associated comorbidity, convenience sample

Exclusion Criteria:

  • Women who have undergone bariatric surgery, menopause, cancer or any metabolic disease, smokers, alcoholics, who are in use of drugs that act directly on the metabolism and that have medical impediment to the practice of physical exercise.

Studieplan

Det här avsnittet ger detaljer om studieplanen, inklusive hur studien är utformad och vad studien mäter.

Hur är studien utformad?

Designdetaljer

  • Primärt syfte: Behandling
  • Tilldelning: N/A
  • Interventionsmodell: Enskild gruppuppgift
  • Maskning: Ingen (Open Label)

Vapen och interventioner

Deltagargrupp / Arm
Intervention / Behandling
Övrig: Physical Training

There was concurrent physical training intervention: strength and aerobic exercises in the same session.

Duration: 2 weeks of adaptation and learning to exercise, 8 weeks of physical training.

Frequency: 3 times per week Duration: 55 minutes each session. Intensity: 75 to 90% of maximum heart rate.
Övrig: Physical Training

Intervention with concurrent physical training: strength and aerobic exercises in the same session.

Duration: 2 weeks of adaptation to physical exercise, 8 weeks of training. Frequency: 3 times a week. Time: 55 minutes each session. Intensity: 75 to 90% of maximum heart rate.

Vad mäter studien?

Primära resultatmått

Resultatmått
Åtgärdsbeskrivning
Tidsram
Changes Body weight
Tidsram: Two times: (1) First day and (2) 10 weeks after adaptation and intervention
Body weight was measured by digital balance before and after the intervention
Two times: (1) First day and (2) 10 weeks after adaptation and intervention
Changes Body composition
Tidsram: Two times: (1) First day and (2) 10 weeks after adaptation and intervention
The change in body composition through deuterium oxide was evaluated.
Two times: (1) First day and (2) 10 weeks after adaptation and intervention
Changes White adipose tissue biopsy
Tidsram: Two times: (1) First day and (2) 10 weeks after adaptation and intervention
A subcutaneous tissue sample was collected for analysis of: mitochondrial respiration, citrate synthase enzyme, gene expression (UCP1, 2 and 3).
Two times: (1) First day and (2) 10 weeks after adaptation and intervention
Changes Indirect calorimetry
Tidsram: Two times: (1) First day and (2) 10 weeks after adaptation and intervention
With a gas analyzer (indirect calorimeter), we evaluated the metabolic rate and rest (REE) and oxidation of substrates (Lipids and carbohydrates).
Two times: (1) First day and (2) 10 weeks after adaptation and intervention
Changes in fatty acids
Tidsram: Two times: (1) First day and (2) 10 weeks after adaptation and intervention
Collected in lithium heparin tubes, they were centrifuged.
Two times: (1) First day and (2) 10 weeks after adaptation and intervention
Changes oxidative stress
Tidsram: Two times: (1) First day and (2) 10 weeks after adaptation and intervention
Collected in lithium heparin tubes, they were centrifuged.
Two times: (1) First day and (2) 10 weeks after adaptation and intervention
Changes inflammatory cytokines
Tidsram: Two times: (1) First day and (2) 10 weeks after adaptation and intervention
Collected in lithium heparin tubes, they were centrifuged.
Two times: (1) First day and (2) 10 weeks after adaptation and intervention
Changes in total cholesterol
Tidsram: Two times: (1) First day and (2) 10 weeks after adaptation and intervention
Collected in lithium heparin tubes, they were centrifuged.
Two times: (1) First day and (2) 10 weeks after adaptation and intervention
Changes Physical Performance
Tidsram: Two times: (1) First day and (2) 10 weeks after adaptation and intervention
Based on the Shuttle Walking Test adaptation.
Two times: (1) First day and (2) 10 weeks after adaptation and intervention
Changes in Determination of Lactate
Tidsram: Two times: (1) First day and (2) 10 weeks after adaptation and intervention
Blood samples were collected by manual puncture of the earlobe in previously calibrated and heparinized capillary tubes, stored in eppendorf with sodium fluoride. Analyzed by electrochemical lactate analyser.
Two times: (1) First day and (2) 10 weeks after adaptation and intervention
Changes Food intake
Tidsram: Two times: (1) First day and (2) 10 weeks after adaptation and intervention
Food registry of 3 days, the quantification of the daily intake of nutrients will still be made using software.
Two times: (1) First day and (2) 10 weeks after adaptation and intervention
Changes Nitrogen Balance
Tidsram: Two times: (1) First day and (2) 10 weeks after adaptation and intervention
Through the collection of urine of 24 hours the dosage of urinary nitrogen will be made by the chemiluminescence method for determination of protein nitrogen.
Two times: (1) First day and (2) 10 weeks after adaptation and intervention
Changes Telomere length
Tidsram: Two times: (1) First day and (2) 10 weeks after adaptation and intervention
peripheral blood in ethylenediaminetetraacetic acid tubes and genomic DNA was automatically extracted from Peripheral Blood Mononuclear Cell. The relative quantification of Telomere length was determined using the telomere to single copy gene ratio by Quantitative Polymerase Chain Reaction (qPCR).
Two times: (1) First day and (2) 10 weeks after adaptation and intervention

Samarbetspartners och utredare

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Sponsor

University of Sao Paulo

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Studieavstämningsdatum

Dessa datum spårar framstegen för inlämningar av studieposter och sammanfattande resultat till ClinicalTrials.gov. Studieposter och rapporterade resultat granskas av National Library of Medicine (NLM) för att säkerställa att de uppfyller specifika kvalitetskontrollstandarder innan de publiceras på den offentliga webbplatsen.

Studera stora datum

Studiestart (Faktisk)

1 april 2016

Primärt slutförande (Faktisk)

1 juli 2016

Avslutad studie (Faktisk)

15 september 2016

Studieregistreringsdatum

Först inskickad

10 april 2017

Först inskickad som uppfyllde QC-kriterierna

13 april 2017

Första postat (Faktisk)

18 april 2017

Uppdateringar av studier

Senaste uppdatering publicerad (Faktisk)

30 april 2019

Senaste inskickade uppdateringen som uppfyllde QC-kriterierna

27 april 2019

Senast verifierad

1 april 2019

Mer information

Termer relaterade till denna studie

Ytterligare relevanta MeSH-villkor

Andra studie-ID-nummer

  • Process HCRP: 1.387.040/2016

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