- ICH GCP
- US Clinical Trials Registry
- Klinisk forsøg NCT03119350
Mitochondrial Energy Metabolism in Obese Women
Mitochondrial Energy Metabolism in Obese Women Undergoing Concurrent Physical Training
Considering that the failure of the treatment of obesity is justified by the multifactorial pathophysiology of this morbidity, the present project has the following hypotheses:
- The occurrence of obesity is due to the derange,ent of mitochondrial energy metabolism ;
- The unbalance is therapeutically modified through physical training ;
- Obesity courses with the break-down in energy metabolism mitochondrial disease associated with systemic inflammatory characteristics that can be corrected through a combined long-term physical training program.
This study have as objective : to analyse changes in mitochondrial function, inflammatory profile, oxidative stress and energy metabolism caused by concurrent physical training in obese women.
Studieoversigt
Status
Intervention / Behandling
Detaljeret beskrivelse
Specific objectives:
Body composition by deuterium oxide; Metabolic rate of resting and oxidation of substrates by indirect calorimetry; Proinflammatory cytokines Anti-inflammatory cytokines Oxidative Stress: Malondialdehyde, Superoxide Dismutase, Glutathione-Peroxidase; Fatty acids: ceramide and palmitate; Mitochondrial respiration and citrate synthase enzyme; Quantify and qualify: mitochondrial number, endoplasmic reticulum structure, adipose cell size; Gene expression, quantify by microscopy and analyze the protein by western blot.
The study began with 20 women, however, there was withdrawal of 6, ending with 14 women.
Undersøgelsestype
Tilmelding (Faktiske)
Fase
- Ikke anvendelig
Kontakter og lokationer
Studiesteder
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SP
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Ribeirao Preto, SP, Brasilien, 14.048-900
- Camila Fernanda Cunha Brandão
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Deltagelseskriterier
Berettigelseskriterier
Aldre berettiget til at studere
Tager imod sunde frivillige
Køn, der er berettiget til at studere
Beskrivelse
Inclusion Criteria:
- This study included women with obesity (BMI of 30 to 40 kg / m²), sedentary, with no associated comorbidity, convenience sample
Exclusion Criteria:
- Women who have undergone bariatric surgery, menopause, cancer or any metabolic disease, smokers, alcoholics, who are in use of drugs that act directly on the metabolism and that have medical impediment to the practice of physical exercise.
Studieplan
Hvordan er undersøgelsen tilrettelagt?
Design detaljer
- Primært formål: Behandling
- Tildeling: N/A
- Interventionel model: Enkelt gruppeopgave
- Maskning: Ingen (Åben etiket)
Våben og indgreb
Deltagergruppe / Arm |
Intervention / Behandling |
---|---|
Andet: Physical Training
There was concurrent physical training intervention: strength and aerobic exercises in the same session. Duration: 2 weeks of adaptation and learning to exercise, 8 weeks of physical training. Frequency: 3 times per week Duration: 55 minutes each session. Intensity: 75 to 90% of maximum heart rate. |
Intervention with concurrent physical training: strength and aerobic exercises in the same session. Duration: 2 weeks of adaptation to physical exercise, 8 weeks of training. Frequency: 3 times a week. Time: 55 minutes each session. Intensity: 75 to 90% of maximum heart rate. |
Hvad måler undersøgelsen?
Primære resultatmål
Resultatmål |
Foranstaltningsbeskrivelse |
Tidsramme |
---|---|---|
Changes Body weight
Tidsramme: Two times: (1) First day and (2) 10 weeks after adaptation and intervention
|
Body weight was measured by digital balance before and after the intervention
|
Two times: (1) First day and (2) 10 weeks after adaptation and intervention
|
Changes Body composition
Tidsramme: Two times: (1) First day and (2) 10 weeks after adaptation and intervention
|
The change in body composition through deuterium oxide was evaluated.
|
Two times: (1) First day and (2) 10 weeks after adaptation and intervention
|
Changes White adipose tissue biopsy
Tidsramme: Two times: (1) First day and (2) 10 weeks after adaptation and intervention
|
A subcutaneous tissue sample was collected for analysis of: mitochondrial respiration, citrate synthase enzyme, gene expression (UCP1, 2 and 3).
|
Two times: (1) First day and (2) 10 weeks after adaptation and intervention
|
Changes Indirect calorimetry
Tidsramme: Two times: (1) First day and (2) 10 weeks after adaptation and intervention
|
With a gas analyzer (indirect calorimeter), we evaluated the metabolic rate and rest (REE) and oxidation of substrates (Lipids and carbohydrates).
|
Two times: (1) First day and (2) 10 weeks after adaptation and intervention
|
Changes in fatty acids
Tidsramme: Two times: (1) First day and (2) 10 weeks after adaptation and intervention
|
Collected in lithium heparin tubes, they were centrifuged.
|
Two times: (1) First day and (2) 10 weeks after adaptation and intervention
|
Changes oxidative stress
Tidsramme: Two times: (1) First day and (2) 10 weeks after adaptation and intervention
|
Collected in lithium heparin tubes, they were centrifuged.
|
Two times: (1) First day and (2) 10 weeks after adaptation and intervention
|
Changes inflammatory cytokines
Tidsramme: Two times: (1) First day and (2) 10 weeks after adaptation and intervention
|
Collected in lithium heparin tubes, they were centrifuged.
|
Two times: (1) First day and (2) 10 weeks after adaptation and intervention
|
Changes in total cholesterol
Tidsramme: Two times: (1) First day and (2) 10 weeks after adaptation and intervention
|
Collected in lithium heparin tubes, they were centrifuged.
|
Two times: (1) First day and (2) 10 weeks after adaptation and intervention
|
Changes Physical Performance
Tidsramme: Two times: (1) First day and (2) 10 weeks after adaptation and intervention
|
Based on the Shuttle Walking Test adaptation.
|
Two times: (1) First day and (2) 10 weeks after adaptation and intervention
|
Changes in Determination of Lactate
Tidsramme: Two times: (1) First day and (2) 10 weeks after adaptation and intervention
|
Blood samples were collected by manual puncture of the earlobe in previously calibrated and heparinized capillary tubes, stored in eppendorf with sodium fluoride.
Analyzed by electrochemical lactate analyser.
|
Two times: (1) First day and (2) 10 weeks after adaptation and intervention
|
Changes Food intake
Tidsramme: Two times: (1) First day and (2) 10 weeks after adaptation and intervention
|
Food registry of 3 days, the quantification of the daily intake of nutrients will still be made using software.
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Two times: (1) First day and (2) 10 weeks after adaptation and intervention
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Changes Nitrogen Balance
Tidsramme: Two times: (1) First day and (2) 10 weeks after adaptation and intervention
|
Through the collection of urine of 24 hours the dosage of urinary nitrogen will be made by the chemiluminescence method for determination of protein nitrogen.
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Two times: (1) First day and (2) 10 weeks after adaptation and intervention
|
Changes Telomere length
Tidsramme: Two times: (1) First day and (2) 10 weeks after adaptation and intervention
|
peripheral blood in ethylenediaminetetraacetic acid tubes and genomic DNA was automatically extracted from Peripheral Blood Mononuclear Cell.
The relative quantification of Telomere length was determined using the telomere to single copy gene ratio by Quantitative Polymerase Chain Reaction (qPCR).
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Two times: (1) First day and (2) 10 weeks after adaptation and intervention
|
Samarbejdspartnere og efterforskere
Sponsor
Publikationer og nyttige links
Generelle publikationer
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- WEIR JB. New methods for calculating metabolic rate with special reference to protein metabolism. J Physiol. 1949 Aug;109(1-2):1-9. doi: 10.1113/jphysiol.1949.sp004363. No abstract available.
- Burgomaster KA, Howarth KR, Phillips SM, Rakobowchuk M, Macdonald MJ, McGee SL, Gibala MJ. Similar metabolic adaptations during exercise after low volume sprint interval and traditional endurance training in humans. J Physiol. 2008 Jan 1;586(1):151-60. doi: 10.1113/jphysiol.2007.142109. Epub 2007 Nov 8.
- Heilbronn LK, Gan SK, Turner N, Campbell LV, Chisholm DJ. Markers of mitochondrial biogenesis and metabolism are lower in overweight and obese insulin-resistant subjects. J Clin Endocrinol Metab. 2007 Apr;92(4):1467-73. doi: 10.1210/jc.2006-2210. Epub 2007 Jan 23.
- Kraunsoe R, Boushel R, Hansen CN, Schjerling P, Qvortrup K, Stockel M, Mikines KJ, Dela F. Mitochondrial respiration in subcutaneous and visceral adipose tissue from patients with morbid obesity. J Physiol. 2010 Jun 15;588(Pt 12):2023-32. doi: 10.1113/jphysiol.2009.184754. Epub 2010 Apr 26. Erratum In: J Physiol. 2010 Oct 15; 588(Pt 20):4055.
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- BROWN, L.E.; WEIR, J.P.; ASEP procedures recommendation i: accurate assessment of muscular strength and power, Journal of Exercise Physiology, v. 4, n. 3, p. 1-21, 2001.
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- Colégio Americano de Medicina Esportiva, CAME. Guia para Teste de Esforço e Prescrição de Exercício. 3º Edição, Medsi, Rio de janeiro, RJ, p.25, 1987.
- Crump C, Sundquist J, Winkleby MA, Sundquist K. Interactive effects of obesity and physical fitness on risk of ischemic heart disease. Int J Obes (Lond). 2017 Feb;41(2):255-261. doi: 10.1038/ijo.2016.209. Epub 2016 Nov 21.
- Curtis JM, Grimsrud PA, Wright WS, Xu X, Foncea RE, Graham DW, Brestoff JR, Wiczer BM, Ilkayeva O, Cianflone K, Muoio DE, Arriaga EA, Bernlohr DA. Downregulation of adipose glutathione S-transferase A4 leads to increased protein carbonylation, oxidative stress, and mitochondrial dysfunction. Diabetes. 2010 May;59(5):1132-42. doi: 10.2337/db09-1105. Epub 2010 Feb 11.
- Daussin FN, Zoll J, Dufour SP, Ponsot E, Lonsdorfer-Wolf E, Doutreleau S, Mettauer B, Piquard F, Geny B, Richard R. Effect of interval versus continuous training on cardiorespiratory and mitochondrial functions: relationship to aerobic performance improvements in sedentary subjects. Am J Physiol Regul Integr Comp Physiol. 2008 Jul;295(1):R264-72. doi: 10.1152/ajpregu.00875.2007. Epub 2008 Apr 16.
- EIGENTLER, A.; DRAXL, A.; et al. Laboratory Protocol: Citrate synthase a mitochondrial marker enzyme. Mitochondrial Physiology Network, v. 17.04, n. 3, p. 1-11, 2015.
- Fernstrom M, Bakkman L, Loogna P, Rooyackers O, Svensson M, Jakobsson T, Brandt L, Lagerros YT. Improved Muscle Mitochondrial Capacity Following Gastric Bypass Surgery in Obese Subjects. Obes Surg. 2016 Jul;26(7):1391-7. doi: 10.1007/s11695-015-1932-z.
- Ferreira FC, Bertucci DR, Barbosa MR, Nunes JE, Botero JP, Rodrigues MF, Shiguemoto GE, Santoro V, Verzola AC, Nonaka RO, Verzola RM, Baldissera V, Perez SE. Circuit resistance training in women with normal weight obesity syndrome: body composition, cardiometabolic and echocardiographic parameters, and cardiovascular and skeletal muscle fitness. J Sports Med Phys Fitness. 2017 Jul-Aug;57(7-8):1033-1044. doi: 10.23736/S0022-4707.16.06391-X. Epub 2016 Jul 6.
- FETT, C.A.; FETT, W.C.R.; MARCHINI, J.S. Fitness Level of Overweight/Obese Women After 08 Weeks of Aerobic or Mixed Metabolism Exercises. Revista Brasileira de Cineantropometria & Desempenho Humano, v. 11, p. 261-266, 2009.
- Grimble GK, West MF, Acuti AB, Rees RG, Hunjan MK, Webster JD, Frost PG, Silk DB. Assessment of an automated chemiluminescence nitrogen analyzer for routine use in clinical nutrition. JPEN J Parenter Enteral Nutr. 1988 Jan-Feb;12(1):100-6. doi: 10.1177/0148607188012001100.
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- Kong Z, Sun S, Liu M, Shi Q. Short-Term High-Intensity Interval Training on Body Composition and Blood Glucose in Overweight and Obese Young Women. J Diabetes Res. 2016;2016:4073618. doi: 10.1155/2016/4073618. Epub 2016 Sep 28.
- Lepage G, Roy CC. Improved recovery of fatty acid through direct transesterification without prior extraction or purification. J Lipid Res. 1984 Dec 1;25(12):1391-6.
- Medbo JI, Mamen A, Holt Olsen O, Evertsen F. Examination of four different instruments for measuring blood lactate concentration. Scand J Clin Lab Invest. 2000 Aug;60(5):367-80. doi: 10.1080/003655100750019279.
- Pfrimer K, Moriguti JC, Lima NK, Marchini JS, Ferriolli E. Bioelectrical impedance with different equations versus deuterium oxide dilution method for the inference of body composition in healthy older persons. J Nutr Health Aging. 2012 Feb;16(2):124-7. doi: 10.1007/s12603-011-0137-y.
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Datoer for undersøgelser
Studer store datoer
Studiestart (Faktiske)
Primær færdiggørelse (Faktiske)
Studieafslutning (Faktiske)
Datoer for studieregistrering
Først indsendt
Først indsendt, der opfyldte QC-kriterier
Først opslået (Faktiske)
Opdateringer af undersøgelsesjournaler
Sidste opdatering sendt (Faktiske)
Sidste opdatering indsendt, der opfyldte kvalitetskontrolkriterier
Sidst verificeret
Mere information
Begreber relateret til denne undersøgelse
Yderligere relevante MeSH-vilkår
Andre undersøgelses-id-numre
- Process HCRP: 1.387.040/2016
Plan for individuelle deltagerdata (IPD)
Planlægger du at dele individuelle deltagerdata (IPD)?
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