- ICH GCP
- US Clinical Trials Registry
- Klinisk utprøving NCT00403598
Preliminary Study for Identification of Calcium-Binding Proteins in the Serum in Various Metabolic Bone Disorders
Studieoversikt
Status
Detaljert beskrivelse
Osteoporosis is an important and costly health issue. The diagnosis of the disease is based on measurement of bone mineral density or the occurrence of characteristic fractures. Currently it is not possible to predict fractures in individual patients, and the ability to monitor the response to treatment is limited as well.
We have synthesized and characterized a novel photoreactive Ca2+-analog reagent, Azido ruthenium (AzRu) that binds covalently to Ca2+-binding sites in proteins. The specificity of AzRu for Ca2+-binding proteins, its photoactivation and its radioactive labeling pave the way for a wide use of AzRu as a powerful tool to assess Ca2+-dependent cellular processes, both in vitro and in vivo. Using radiolabeled AzRu, it will be possible to identify Ca2+-binding proteins in a biological sample such as whole cell, isolated mitochondria, ER or other protein containing fractions. Protein bound [103Ru]AzRu in biological sample can be separated by SDS gel electrophoresis, followed by Coomassie staining and exposure of the dried gel to X-ray film (autoradiography). This allows the identification of the [103Ru] radiolabeled proteins. The labeled protein bands can be cut from the gel and subjected to MALADI-TOF analysis and the identity of the protein can be determined by a sequence homology search.
These would allow detection of Ca2+-binding proteins in the serum of various diseases and disorders, potentially leading to identification of novel biomarkers for disease diagnostics.
We would like to use blood samples to carry out the procedure described above for identification of Ca2+ binding proteins appearing or their level is increased in disease or under pathogenic conditions. Since Ca2+ binding proteins play an important role in bone metabolism, we chose to test their occurrence in the serum of patients with osteoporosis and other metabolic bone diseases.
Studietype
Kontakter og plasseringer
Studiekontakt
- Navn: Iris Vered, MD
- Telefonnummer: 972-3-5303103
- E-post: iris.vered@sheba.health.gov.il
Studer Kontakt Backup
- Navn: Varda Shoshan-Barmatz, Ph.D
- Telefonnummer: 972-8-6461336
- E-post: vardasb@bgu.ac.il
Studiesteder
-
-
-
Ramat Gan, Israel, 52621
- Sheba Medical Center, Tel Hashomer
-
Hovedetterforsker:
- Iris Vered, M.D
-
-
Deltakelseskriterier
Kvalifikasjonskriterier
Alder som er kvalifisert for studier
Tar imot friske frivillige
Kjønn som er kvalifisert for studier
Beskrivelse
Inclusion Criteria:
- Clinical diagnosis of a metabolic bone disorder
Exclusion Criteria:
- none
Studieplan
Hvordan er studiet utformet?
Designdetaljer
Samarbeidspartnere og etterforskere
Sponsor
Samarbeidspartnere
Etterforskere
- Hovedetterforsker: Iris Vered, M.D, Sheba Medical Center, Tel Hashomer
Studierekorddatoer
Datoer for studieregistrering
Først innsendt
Først innsendt som oppfylte QC-kriteriene
Først lagt ut (Anslag)
Oppdateringer av studieposter
Sist oppdatering lagt ut (Anslag)
Siste oppdatering sendt inn som oppfylte QC-kriteriene
Sist bekreftet
Mer informasjon
Begreper knyttet til denne studien
Ytterligere relevante MeSH-vilkår
Andre studie-ID-numre
- SHEBA-06-4372-IV-CTIL
Denne informasjonen ble hentet direkte fra nettstedet clinicaltrials.gov uten noen endringer. Hvis du har noen forespørsler om å endre, fjerne eller oppdatere studiedetaljene dine, vennligst kontakt register@clinicaltrials.gov. Så snart en endring er implementert på clinicaltrials.gov, vil denne også bli oppdatert automatisk på nettstedet vårt. .
Kliniske studier på Parathyreoidea sykdommer
-
Erhan AysanFullført
-
SB Istanbul Education and Research HospitalUkjent
-
Fujian Medical UniversityUkjentIntraoperativt parathyroid scoresystemKina
-
University of East AngliaMinistry of Defence, United Kingdom; Clinical Research and Trials Unit... og andre samarbeidspartnereUkjentStressbrudd | Parathyroid hormonStorbritannia
-
Fujian Medical UniversityFullførtIntraoperativt parathyroid scoresystemKina
-
M.D. Anderson Cancer CenterRekruttering
-
University of ArkansasFullførtParathyroid sykdomForente stater
-
SB Istanbul Education and Research HospitalBezmialem Vakif UniversityUkjentParathyroid allotransplantasjonTyrkia
-
University of ArkansasFullførtKirurg sammenlignet med nukleær radiologilesere for Tc-99m Sestamibi-skanning for parathyroid sykdomParathyroid sykdomForente stater
-
Sisli Hamidiye Etfal Training and Research HospitalBezmialem Vakif UniversityUkjent