Quantification of Proteomes: L-plastin , Lipocalin and Adiponectin in Chronic Periodontitis and Diabetic Subjects
2017年12月29日 更新者:Dr.Jaideep Mahendra、Meenakshi Ammal Dental College and Hospital
Quantification of Proteomes: L-plastin , Lipocalin and Adiponectin in Subgingival Tissue Samples of Generalized Severe Chronic Periodontitis Patients With and Without Type II Diabetes Mellitus
The quantification of the proteomes : L-plastin, lipocalin and adiponectin was analysed in the subgingival tissue samples of generalized severe chronic periodontitis patients with and without diabetes mellitus.The demographic and the periodontal parameters were assessed and the correlated with the quantification of the proteins.
The mRNA expression was analyzed with RT-PCR and the quantification of protein was done using ELISA.
研究概览
地位
完全的
条件
详细说明
The quantification of the proteomes : L-plastin, lipocalin and adiponectin was analysed in the subgingival tissue samples of generalized severe chronic periodontitis patients with and without diabetes mellitus.A total of 70 subjects with generalized severe chronic periodontitis were selected and divided into two groups.
Group I comprised of 35 subjects who were diagnosed with generalized severe chronic periodontitis without any systemic conditions.
Group II comprised of generalized severe chronic periodontitis patients with type II diabetes mellitus.
Demographic variables such as age, weight, height, BMI and income and the clinical parameters such as plaque index, bleeding on probing, probing pocket depth and clinical attachment level were recorded.
Subgingival tissue samples were collected from both the groups from the periodontal surgical site.
The collected samples were subjected to further molecular analysis for the mRNA expression of L-plastin, lipocalin and adiponectin using RT-PCR and for their protein quantification using ELISA.The elevated levels of mRNA expression and elevated quantification of L-plastin and Lipocalin proteins in the subgingival tissue samples of generalized severe chronic periodontitis subjects with diabetes denoted the localized involvement of these molecules in the pathogenesis of both periodontitis and type II diabetes mellitus.
Since adiponectin was reduced in periodontitis patients with type II diabetes mellitus, it can be used as an anti-inflammatory and anti-diabetic biomarker for the early identification of patients at risk for diabetes mellitus and periodontitis.
研究类型
观察性的
注册 (实际的)
70
参与标准
研究人员寻找符合特定描述的人,称为资格标准。这些标准的一些例子是一个人的一般健康状况或先前的治疗。
资格标准
适合学习的年龄
35年 至 60年 (成人)
接受健康志愿者
不
有资格学习的性别
男性
取样方法
非概率样本
研究人群
All male patients within the age group of 35-60 years.
The subjects were from the South Indian population.
描述
Inclusion Criteria:
- Patients willing to participate in the study.
- Male patients within the age group 35 to 60 years.
- Patients having ≥ 10 natural teeth.
- No history of long term antibiotic use in the past 6 months and no history of diabetes mellitus.
For Group I:
• Generalized severe chronic periodontitis subjects (30% or more sites with clinical attachment loss (CAL) ≥ 5mm) without type II diabetes mellitus.
For Group II:
• Generalized severe chronic periodontitis subjects (30% or more sites with clinical attachment loss (CAL) ≥ 5mm) diagnosed with type II diabetes mellitus (HbA1C levels ≥7%) for the past 5 years and is under oral medication.
Exclusion Criteria:
- Patients with systemic conditions, such as cardiovascular and respiratory diseases, HIV infection, immunosuppressive patients and patients under chemotherapy.
- Smokers and other behavioral factors which may influence the study.
- Patients who had undergone periodontal therapy within the previous 6 months.
Female patients (due to hormonal changes and pregnancy which may alter the oral flora).
-
学习计划
本节提供研究计划的详细信息,包括研究的设计方式和研究的衡量标准。
研究是如何设计的?
设计细节
- 观测模型:其他
- 时间观点:其他
队列和干预
团体/队列 |
---|
Group I
GROUP I: 35 generalized severe chronic periodontitis subjects without type II diabetes mellitus and systemically healthy.
|
Group II
GROUP II: 35 generalized severe chronic periodontitis subjects diagnosed with type II diabetes mellitus.
|
研究衡量的是什么?
主要结果指标
结果测量 |
措施说明 |
大体时间 |
---|---|---|
mRNA expression of L-plastin,lipocalin and adiponection
大体时间:9 months
|
mRNA expression of L-plastin,lipocalin and adiponectin using Real Time PCR
|
9 months
|
次要结果测量
结果测量 |
措施说明 |
大体时间 |
---|---|---|
Quantify the levels of L-plastin, lipocalin and adiponectin
大体时间:9 months
|
Quantify levels of L-plastin, lipocalin and adiponectin using ELISA.
|
9 months
|
合作者和调查者
在这里您可以找到参与这项研究的人员和组织。
调查人员
- 研究主任:Jaideep Mahendra, MDS,PhD、Meenakshi Ammal Dental College
出版物和有用的链接
负责输入研究信息的人员自愿提供这些出版物。这些可能与研究有关。
研究记录日期
这些日期跟踪向 ClinicalTrials.gov 提交研究记录和摘要结果的进度。研究记录和报告的结果由国家医学图书馆 (NLM) 审查,以确保它们在发布到公共网站之前符合特定的质量控制标准。
研究主要日期
学习开始 (实际的)
2016年10月20日
初级完成 (实际的)
2017年7月22日
研究完成 (实际的)
2017年12月1日
研究注册日期
首次提交
2017年12月21日
首先提交符合 QC 标准的
2017年12月29日
首次发布 (实际的)
2018年1月5日
研究记录更新
最后更新发布 (实际的)
2018年1月5日
上次提交的符合 QC 标准的更新
2017年12月29日
最后验证
2017年12月1日
更多信息
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