子宫内膜异位症转录组细胞图谱 (EDISON)
建立子宫内膜异位症转录组细胞图谱以破译干细胞和雌激素在该疾病中的病理生理作用
Endometriosis is an estrogen-dependent, chronic inflammatory gynecologic disease affecting women of reproductive age, with a therapeutic wandering of 6 to 10 years. A better understanding of the initiation phase is a major challenge to improve diagnosis and treatment. The most widely accepted hypothesis to explain the formation of endometriotic lesions is the tubal retrograde reflux during menstruation. However, only 10% of the reproductive age women will develop endometriosis while 90% of women experience retrograde menstruation. This raises the question of the stem cells present in the endometrium and menstrual reflux of these patients, but also of both the peritoneal microenvironment and the estrogenic local signaling which allow the implantation of these lesions.
研究概览
详细说明
Using tissues collected at different phases of menstrual cycle in healthy women and patients operated for endometriotic lesions, 3 main objectives :
- To characterize cellular heterogeneity between eutopic endometrium and ectopic lesions in parallel with peritoneal fluid to identify potential stem cells and the immune microenvironment in order to find new biomarkers (using a combination of unbiased transcriptomic, spectral flow cytometry and multiplex imaging analysis)
- To develop organoid models that integrate peritoneal fluid elements (supernatant and/or cells) to clarify stem cell properties/characteristics and their supportive environment
- To leverage the use of these endometrial derived-organoids to functionally study the differential influence of estrogen signaling in women with or without endometriosis
研究类型
注册 (估计的)
阶段
- 不适用
联系人和位置
学习联系方式
- 姓名：Elodie CHANTALAT, MD
- 电话号码：+33 0561323953
- 邮箱：chantalat.e@chu-toulouse.fr
研究联系人备份
- 姓名：Françoise LENFANT, PhD
- 邮箱：francoise.lenfant@inserm.fr
学习地点
-
-
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Toulouse、法国
- 招聘中
- University Hospital Toulouse
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接触：
- Elodie CHANTALAT, MD
- 邮箱：chantalat.e@chu-toulouse.fr
-
首席研究员：
- Elodie CHANTALAT, MD
-
-
参与标准
资格标准
适合学习的年龄
接受健康志愿者
有资格学习的性别
描述
Inclusion Criteria:
- Patients over 18 and under 45 years of age
- Patients with multiple endometriosis sites (superficial, deep and endometriomas) with an indication for surgery to treat disabling painful symptoms and stage III or IV endometriosis in the context of infertility.
- Healthy patients including those operated on between the ages of 18 and 45 for tubal ligation
- Healthy patients operated on between the ages of 18 and 45 for hysterectomies for benign causes other than endometriosis or adenomyosis
- Patients operated on strictly at the time of menstruation
- Participants who have not been on hormonal treatment for 2 months
- Participant having signed the free and informed consent form
- Membership of a Social Security scheme or equivalent
Exclusion Criteria:
- Patients with endometriosis non-surgically treated
- Patients with endometriosis receiving hormonal treatment at the time of surgery or having received hormonal treatment in the 2 months prior to surgery
- Patients with adenomyosis only
- Healthy non-endometriotic patients undergoing hysterectomy for infectious or malignant causes
- Patients benefiting from a legal protection measure (guardianship, curatorship, safeguard of justice)
- Patients with positive serology for human immunodeficiency virus (HIV), human T-lymphotropic virus (HTLV), hepatitis B (HBV) and hepatitis C (HCV)
学习计划
研究是如何设计的？
设计细节
- 主要用途：其他
- 分配：非随机化
- 介入模型：并行分配
- 屏蔽：无（打开标签）
武器和干预
参与者组/臂 |
干预/治疗 |
|---|---|
|
其他：Patient with endometriosis
Endometriosis patients will be operated on as part of their pathology. |
Anatomopathology waste will be recovered, i.e. the remainder of the endometriosis lesions and the remainder of the endometrial biopsy. A blood sample will be taken the day before surgery, as part of the treatment, for a hormonal check-up. |
|
其他：Healthy control patient
Healthy patients will be operated for tubal ligation or hysterectomies. |
During surgery, an endometrial biopsy will be taken for research purposes. A blood sample will be taken the day before surgery, as part of the treatment, for a hormonal check-up. |
研究衡量的是什么？
主要结果指标
结果测量 |
大体时间 |
|---|---|
|
分析健康对照患者子宫内膜中存在的不同细胞类型（免疫细胞和子宫内膜干细胞）的数量
大体时间：手术期间
|
手术期间
|
|
分析健康对照患者腹腔液中存在的不同细胞类型（免疫细胞和子宫内膜干细胞）的数量
大体时间：手术期间
|
手术期间
|
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分析子宫内膜异位症患者子宫内膜异位症病变中存在的不同细胞类型（免疫细胞和子宫内膜干细胞）的数量。
大体时间：手术期间
|
手术期间
|
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分析子宫内膜异位症患者子宫内膜中存在的不同细胞类型（免疫细胞和子宫内膜干细胞）的数量。
大体时间：手术期间
|
手术期间
|
合作者和调查者
调查人员
- 首席研究员：Elodie CHANTALAT, MD、University Hospital, Toulouse
研究记录日期
研究主要日期
学习开始 (实际的)
初级完成 (估计的)
研究完成 (估计的)
研究注册日期
首次提交
首先提交符合 QC 标准的
首次发布 (实际的)
研究记录更新
最后更新发布 (实际的)
上次提交的符合 QC 标准的更新
最后验证
更多信息
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