Muscle Metabolism and Oxygenation During Localized Fatigue-exercise in COPD

Muscle Oxygenation and Metabolism in Response to a Localized Fatigue-inducing Exercise in Patients With Chronic Obstructive Pulmonary Disease

The purpose of this study is to investigate whether a localized exercise, in which cardiorespiratory demand is reduced, will result in greater limb muscle fatigue in patients with COPD as a consequence of muscle oxygenation and muscle metabolism disturbances.

Study Overview

• Status: Unknown
• Conditions: Pulmonary Disease, Chronic Obstructive
• Intervention / Treatment: Other: Fatiguing exercise

• Study Type: Interventional
• Enrollment (Anticipated): 20
• Phase: Not Applicable

Contacts and Locations

• Study Contact: Name: Fernanda Ribeiro, PhD candidate; Phone Number: 3928 418-656-8711; Email: fernanda.ribeiro@criucpq.ulaval.ca

Study Locations

• Canada
  • Quebec
    • Québec, Quebec, Canada, G1V 4G5
      • Recruiting
      • Institut Universitaire de Cardiologie et de Pneumologie de Quebec
      • Principal Investigator: François Maltais, MD
      • Sub-Investigator: Didier Saey, PhD
      • Sub-Investigator: Fernanda Ribeiro, PhD candidate

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 50 years to 75 years (Adult, Older Adult)
• Accepts Healthy Volunteers: Yes
• Genders Eligible for Study: All

Description

Inclusion Criteria:

• COPD (FEV1/FVC < 0.70 and FEV1 < 0.80 predicted
• Healthy subjects: no evidence of airways obstruction (FEV1/FVC > 0.70 and FEV1 > 0.80 predicted)
• Smoking history > 15 pack-years

Exclusion Criteria:

• Chronic hypoxemia and/or hypercapnia (PaO2 < 60 mmHg or SpO2 < 88% at rest and/or PaCO2 > 45 mmHg)
• Recent exacerbation (< 3 months)
• Recent cancer (< 3 years)
• Diabetes
• Myopathy, neuromuscular or articular disease
• Unstable cardiac disease
• Absolute contraindications to exercise testing
• Regular physical activity or exercise training program in the last 2 months
• Physical activity score > 9 in the Voorips questionnaire
• Thigh skinfold thickness > 15 mm
• BMI > 30

Study Plan

How is the study designed?

Design Details

• Primary Purpose: Basic Science
• Allocation: Randomized
• Interventional Model: Crossover Assignment
• Masking: Single

Number of Arms

2

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
Experimental: Oxygen	Other: Fatiguing exercise; All participants (patients and healthy subjects) will perform the exercise both under normoxic (FiO2 = 0.21) and hyperoxic conditions (FiO2 = 100%)
Sham Comparator: Room air	Other: Fatiguing exercise; All participants (patients and healthy subjects) will perform the exercise both under normoxic (FiO2 = 0.21) and hyperoxic conditions (FiO2 = 100%)

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Muscle deoxygenation	Changes of deoxyhemoglobin/myoglobin concentrations measured by near-infrared spectroscopy of vastus lateralis muscle.	45 seconds post-exercise (knee-extension repetitions)
Muscle metabolism	Changes in glycolytic and oxidative metabolism pathway markers, energy substrates (glycogen and glucose), end-products of glycolysis (pyruvate, and lactate), intermediate markers of glycolysis and high-energy phosphate compounds obtained from muscle biopsies	45 seconds post-exercise (knee extension repetitions)

Secondary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Ventilatory response	Minute ventilation will be monitored during the fatigue-inducing exercise and it will be compared between subjects and conditions (FiO2 = 21% vs. FiO2 = 100%)	Baseline, beginning and end of each serie of knee extensions (fatiguing exercise)
Quadriceps muscle fatigue	Quadriceps muscle fatigue will be determined by loss of quadriceps strength measured by magnetic stimulation after the fatigue-inducing exercise. Surface electromyography will also be measured to characterize muscle fatigue.	baseline, 15 min and 40 min after the fatigue-inducing exercise
total muscle work	Total muscle work performed during fatigue-inducing exercise that will be composed by subsequent isokinetic knee-extensions repetitions at 40% of maximal peak torque	end of fatiguing exercise

Collaborators and Investigators

• Sponsor: Laval University
• Collaborators: Canadian Lung Association
• Investigators: Principal Investigator: François Maltais, MD, Institut universitaire de cardiologie et de pneumologie de Québec, University Laval

Study record dates

Study Major Dates

• Study Start: September 1, 2011
• Primary Completion (Anticipated): July 1, 2014

Study Registration Dates

• First Submitted: July 16, 2013
• First Submitted That Met QC Criteria: July 23, 2013
• First Posted (Estimate): July 26, 2013

Study Record Updates

• Last Update Posted (Estimate): February 7, 2014
• Last Update Submitted That Met QC Criteria: February 6, 2014
• Last Verified: February 1, 2014

More Information

Terms related to this study

• Keywords: Pulmonary Disease, Chronic Obstructive; Energy metabolism; Muscle oxygenation; Muscle fatigue
• Additional Relevant MeSH Terms: Pathologic Processes; Respiratory Tract Diseases; Disease Attributes; Lung Diseases, Obstructive; Fatigue; Lung Diseases; Pulmonary Disease, Chronic Obstructive; Chronic Disease
• Other Study ID Numbers: MX-20665