Toxicity of Perirenal Fat in Overweight or Obese Subjects: A Pathophysiological Link Between Uric Acid Stones and Renal Ammonium Formation

Patients who are overweight or obese, diabetic or not, share with those who are suffering from uric stones the same way to remove abnormal acidity of the body in urine, ie a kidney ammoniogenesis default. This results in an overly acidic urine pH which is directly pathogenic in people predisposed to develop uric stones because the precipitation of urate soluble uric acid is accelerated in acid medium.

Excess visceral fat, particularly perirenal, this defect may promote formation of renal ammonium. Indeed, the perirenal fat is adjacent to the renal cortex and shares with it a common arterial supply via the plexus Turner. Adipokines and fatty acids of the perirenal fat are predisposed to gain the renal cortex, seat of the ammoniogenesis. In humans the pathogenic role of the perirenal fat is demonstrated in chronic kidney disease and essential hypertension. However, the amount of fat and perirenal that of intra-abdominal fat are positively correlated.

Investigators hypothesis is that the perirenal fat also exert a pathogenic role in uric because of anatomical links between kidney stones and greasy environment and because excess fatty acids reaching the renal cortex decreases ammoniogenesis in an animal model metabolic syndrome.

For the test, the investigators will compare the amount of fat and perirenal renal ability to form ammonium in patients with uric or calcium lithiasis taking into account the amount of intra-abdominal fat.

Study Overview

• Status: Completed
• Conditions: Uric Acid Stones
• Intervention / Treatment: Other: acid load test

• Study Type: Interventional
• Enrollment (Actual): 20
• Phase: Not Applicable

Contacts and Locations

Study Locations

• France
  • Nice, France, 06000
    • CHU de Nice

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 18 years to 70 years (ADULT, OLDER_ADULT)
• Accepts Healthy Volunteers: No
• Genders Eligible for Study: Male

Description

Inclusion Criteria:

• Urolithiasis Uric defined by more than 90% of uric acid anhydrous and / or dihydrate or calcium lithiasis defined by more than 90% mono calcium oxalate and / or dihydrate, and / or carbapatite and / or brushite;
• Overweight and obesity defined by 25 <BMI <35 kg / m2

Exclusion Criteria:

• staghorn lithiasis, stones struvite or cystine;
• Primary hyperparathyroidism;
• Hyperthyroidism;
• Any form of calcium or uric lithiasis secondary;
• Abnormal kidney structure (cysts, cortical thinning, kidney tumor);

Study Plan

How is the study designed?

Design Details

• Primary Purpose: OTHER
• Allocation: NA
• Interventional Model: SINGLE_GROUP
• Masking: NONE

Number of Arms

1

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
EXPERIMENTAL: acid load test	Other: acid load test

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
the perirenal fat thickness (left)	This is the perirenal fat thickness measured between the left posterior wall of the kidney and the posterior wall of the abdominal cavity on a TDM section in the plane of the left renal vein.	baseline

Secondary Outcome Measures

Outcome Measure	Measure Description	Time Frame
the perirenal fat thickness (right)	the average thickness of perirenal fat measured to the right	baseline
NH4 + / NAE	It is the ratio NH4 + / NAE measured over 24 hours of urine collected at day 7 of a standard diet; it determines from acid removed as ammonium whose production is regulated physiologically by the kidneys	at day 7 of a standard diet
net acid urinary flow	This is the net acid urinary flow and report NH4 + / creat obtained when the urine pH became <5.5, in an acidic filler.	at day 7 of a standard diet and when urine pH became <5.5

Collaborators and Investigators

• Sponsor: Centre Hospitalier Universitaire de Nice

Study record dates

Study Major Dates

• Study Start (ACTUAL): October 14, 2015
• Primary Completion (ACTUAL): June 24, 2016
• Study Completion (ACTUAL): September 29, 2016

Study Registration Dates

• First Submitted: September 22, 2015
• First Submitted That Met QC Criteria: September 25, 2015
• First Posted (ESTIMATE): September 28, 2015

Study Record Updates

• Last Update Posted (ACTUAL): March 12, 2018
• Last Update Submitted That Met QC Criteria: March 8, 2018
• Last Verified: March 1, 2018

More Information

Terms related to this study

• Keywords: Renal Ammonium ;uric acid
• Additional Relevant MeSH Terms: Kidney Diseases; Urologic Diseases; Body Weight; Urolithiasis; Overweight; Nephrolithiasis
• Other Study ID Numbers: 15-AOI-07