The Effect of Protein Ingestion on the Recovery of Skeletal Muscle Function Following Eccentric Exercise. (SPADE)

The present study will assess the effect of the type and timing of protein ingestion 6 days prior to, and 7 days following eccentric exercise on the time course of 1) the recovery of muscle function, 2) the recovery of perceived muscle soreness, 3) muscle metabolism.

Study Overview

• Status: Completed
• Conditions: Healthy
• Intervention / Treatment: Dietary supplement: Post exercise placebo supplement; Dietary supplement: Pre bed placebo supplement; Dietary supplement: Post exercise protein-polyphenol supplement; Dietary supplement: Pre bed protein-polyphenol supplement

• Study Type: Interventional
• Enrollment (Actual): 36
• Phase: Not Applicable

Contacts and Locations

Study Locations

• United Kingdom
  • Devon
    • Exeter, Devon, United Kingdom, EX1 2LU
      • Sport and Health Sciences

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 18 years to 35 years (Adult)
• Accepts Healthy Volunteers: Yes
• Genders Eligible for Study: All

Description

Inclusion Criteria:

• Men and women who are generally healthy and recreationally active

Exclusion Criteria:

• Active cardiovascular disease or hypertension
• Diagnosed metabolic impairment
• Use of nutritional supplements
• Use of anti-inflammatory medications
• Habitual dietary protein intake of <0.8g/kg/day
• Musculoskeletal disorders

Study Plan

How is the study designed?

Design Details

• Primary Purpose: Basic Science
• Allocation: Randomized
• Interventional Model: Parallel Assignment
• Masking: Triple

Number of Arms

4

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
Placebo Comparator: Placebo supplement; Post exercise placebo supplement Pre bed placebo supplement	Dietary supplement: Post exercise placebo supplement; Participants will ingest an isocaloric placebo supplement after exercise. This will begin 6 days before eccentric exercise and will end 6 days after eccentric exercise.; Dietary supplement: Pre bed placebo supplement; Participants will ingest an isocaloric placebo supplement before bed. This will begin 6 days before eccentric exercise and will end 6 days after eccentric exercise.
Active Comparator: Post exercise supplement; Post exercise protein-polyphenol supplement Pre bed placebo supplement	Dietary supplement: Pre bed placebo supplement; Participants will ingest an isocaloric placebo supplement before bed. This will begin 6 days before eccentric exercise and will end 6 days after eccentric exercise.; Dietary supplement: Post exercise protein-polyphenol supplement; Participants will ingest a post exercise protein-polyphenol supplement. This will begin 6 days before eccentric exercise and will end 6 days after eccentric exercise.
Active Comparator: Pre bed supplement; Post exercise placebo supplement Pre bed protein-polyphenol supplement	Dietary supplement: Post exercise placebo supplement; Participants will ingest an isocaloric placebo supplement after exercise. This will begin 6 days before eccentric exercise and will end 6 days after eccentric exercise.; Dietary supplement: Pre bed protein-polyphenol supplement; Participants will ingest a protein-polyphenol supplement before bed. This will begin 6 days before eccentric exercise and will end 6 days after eccentric exercise.
Active Comparator: Post exercise + pre bed supplement; Post exercise protein-polyphenol supplement Pre bed protein-polyphenol supplement	Dietary supplement: Post exercise protein-polyphenol supplement; Participants will ingest a post exercise protein-polyphenol supplement. This will begin 6 days before eccentric exercise and will end 6 days after eccentric exercise.; Dietary supplement: Pre bed protein-polyphenol supplement; Participants will ingest a protein-polyphenol supplement before bed. This will begin 6 days before eccentric exercise and will end 6 days after eccentric exercise.

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Time Frame
Isokinetic force production of the knee extensor muscles	14 days

Secondary Outcome Measures

Outcome Measure	Time Frame
Muscle protein synthesis	14 days
Thigh volume	14 days
Perceived muscle soreness	14 days
Isometric force production of the knee extensor muscles	14 days

Collaborators and Investigators

• Sponsor: University of Exeter

Publications and helpful links

General Publications

• Jameson TSO, Pavis GF, Dirks ML, Lee BP, Abdelrahman DR, Murton AJ, Porter C, Alamdari N, Mikus CR, Wall BT, Stephens FB. Reducing NF-kappaB Signaling Nutritionally is Associated with Expedited Recovery of Skeletal Muscle Function After Damage. J Clin Endocrinol Metab. 2021 Jun 16;106(7):2057-2076. doi: 10.1210/clinem/dgab106.

Study record dates

Study Major Dates

• Study Start (Actual): January 1, 2017
• Primary Completion (Actual): August 1, 2018
• Study Completion (Actual): August 1, 2018

Study Registration Dates

• First Submitted: November 30, 2016
• First Submitted That Met QC Criteria: November 30, 2016
• First Posted (Estimate): December 2, 2016

Study Record Updates

• Last Update Posted (Actual): October 16, 2018
• Last Update Submitted That Met QC Criteria: October 11, 2018
• Last Verified: October 1, 2018

More Information

Terms related to this study

• Other Study ID Numbers: 181739

Plan for Individual participant data (IPD)

• Plan to Share Individual Participant Data (IPD)?: NO

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: No
• Studies a U.S. FDA-regulated device product: No