Phase I/II Study of Immunotherapy Combination BN-Brachyury Vaccine, M7824, N-803 and Epacadostat (QuEST1)

A Phase I/II Study of Immunotherapy Combination BN-Brachyury Vaccine, M7824, N-803 and Epacadostat (QuEST1)

Background:

Immunotherapy drugs help the body to fight cancer. Scientists think that combining some of these drugs will make them work better than when used alone. This may be true for many types of cancer, including castration-resistant prostate cancer (CRPC).

Objective:

To test if any of the combinations of drugs below have anti-prostate cancer activity and to test if they are safe.

1. Bavarian-Nordic (BN)-brachyury, bintrafusp alfa (M7824).
2. Bavarian-Nordic (BN)-brachyury, bintrafusp alfa (M7824) + Anktiva (N-803).
3. Bavarian-Nordic (BN)-brachyury, bintrafusp alfa (M7824) + Anktiva (N-803) + Epacadostat.

Eligibility:

People ages 18 and older with CRPC or another metastatic cancer

Design:

Participants will be screened with:

• Medical history
• Physical exam
• Computed tomography (CT) or magnetic resonance imaging (MRI) scans
• Possible bone imaging
• Blood, urine, and heart tests
• Possible tumor biopsy

Participants will be treated with a 2-, 3- or 4-drug combinations of the following study drugs in 2-week cycles:

• Participants will receive M7824 by intravenous (IV) once every 2 weeks.
• Participants will receive N-803 by injection once every 2 weeks. They will record any skin changes at the injection site in a diary.
• Participants will receive BN-brachyury as 4 injections to different limbs. They will get the first 3 doses 2 weeks apart. Then they will get doses every 4 weeks for 6 months, then every 3 months for 2 years, then every 6 months.
• Participants will take Epacadostat orally every 12 hours. They will keep a pill diary.

Participants will have physical exams and blood and urine tests at the start of each cycle. They may have scans every 12 weeks.

Participants will continue treatment until their disease gets worse or they cannot tolerate the side effects.

Participants will have a follow-up visit 4-5 weeks after they stop treatment. They will have a physical exam and blood tests. They may be asked to return for scans every 3 months.

Study Overview

• Status: Completed
• Conditions: Solid Tumor; Prostate Cancer; Advanced Solid Tumor; Metastatic Castration-resistant Prostate Cancer; Prostate Neoplasm; Non-metastatic Castration-resistant Prostate Cancer
• Intervention / Treatment: Biological: MVA-BN-Brachyury; Diagnostic test: MRI; Biological: M7824; Drug: N-803; Drug: Acetaminophen; Drug: Ibuprofen; Drug: Naproxen; Other: Benadryl; Diagnostic test: EKG; Diagnostic test: Bone scan; Diagnostic test: CT chest, abdomen and pelvis; Biological: FPV-Brachyury; Drug: Epacadostat

Detailed Description

Background:

• Programmed cell death protein 1 (PD-1)/programmed death ligand 1 (PD-L1) signaling appears to be a major inhibitor of activated T cell anti-tumor immune responses. The rapid, deep and durable responses seen in various malignancies with PD-1/PD-L1 targeted agents demonstrate that blockade of this axis is key to facilitating immune responses within the tumor microenvironment (TME).
• Prostate cancer is poorly recognized by T cells. Lack of an immune response is one explanation for the lower response rates (<15%) observed with anti-PD-1/PD-L1 therapies for prostate cancer.
• Increasing response rates will likely require therapeutic nullification of multiple immune deficits by combining immunotherapies that generate tumor-specific T cells (vaccine), dampen the inhibitory milieu of the TME, and enhance T and natural killer (NK) cell activity within the TME.
• A quick efficacy seeking trial, utilizing sequential arms offers a means to identify signals of activity for combinations of immunotherapy, added sequentially, in castration resistant prostate cancer (CRPC) participants.
• Bavarian Nordic (BN)-Brachyury is a novel recombinant vector-based therapeutic cancer vaccine designed to induce an enhanced immune response against brachyury, which is overexpressed in many solid tumor types, including prostate adenocarcinoma. BN-Brachyury collectively refers to the priming doses (modified vaccinia Ankara (MVA)-BN-Brachyury) and the boost doses (fowlpox virus (FPV)-Brachyury) of the vaccine platform.
• bintrafusp alfa (M7824) is a bifunctional fusion protein consisting of an anti-programmed death ligand 1 (PD-L1) antibody and the extracellular domain of transforming growth factor beta (TGF- beta) receptor type 2, a TGF-beta trap. M7824 can also mediate antibody-dependent cellular cytotoxicity in vitro.
• Anktiva (N-803) is an interleukin 15 (IL-15)/IL-15R alpha superagonist complex that can enhance NK cell mediated antibody-dependent cell-mediated cytotoxicity (ADCC) and T-cell cytotoxicity.
• Synergistic anti-tumor effects have been observed in vitro when combining M7824 and N-803, and in vivo when combining these agents with tumor vaccine in animal models.
• Indoleamine 2,3-dioxygenase 1 (IDO1) is overexpressed in many solid tumors and can contribute to immune escape by tumor cells. INCB024360 (Epacadostat) is an IDO1 inhibitor under investigation in combination with different immunotherapies in treatment of various malignancies.
• In treating of CRPC, we hypothesize that these agents and their effects will be complementary. Tumor-specific T cells generated by vaccine may become more functional in a TME following treatment with M7824 and Epacadostat. N-803 can further enhance the activity of antigen-specific T cells as well as NK cells.

Objective:

-To determine if there is clinical benefit to any of a set of 3 possible treatments for participants with CRPC:

• BN-Brachyury + M7824
• BN-Brachyury + M7824 + N-803
• BN-Brachyury + M7824 + N-803 + Epacadostat

Eligibility:

• Adults with histologically proven CRPC, or metastatic solid tumor of any type for which there is no standard treatment or standard treatment has failed.
• Adequate organ function as defined by liver, kidney, and hematologic laboratory testing.
• Participants with acquired immune defects, active systemic autoimmune disease, history of organ transplant, history of chronic infections, or history of active inflammatory bowel disease are excluded.

Design:

Open label Phase I/II trial with following randomization during the expansion.

Phase I: Cohort 1, Arm 1.1

-Up to 18 participants with any solid tumor will be enrolled in dose escalation Cohort 1 for treatment in Arm 1.1 (flat dose of M7824 + different dose levels of N-803).

Phase IIA: expansion with sequential enrollment into Cohort 2A, Arms 2.1A, 2.2A and 2.3 A

• Concurrently with the enrollment to Arm 1.1, 13 participants with CRPC will start enrollment in Cohort 2A for treatment in Arm 2.1A (M7824 + BN-Brachyury).
• When safe dosing of N-803 is identified during Phase I, 13 participants have enrolled in arm 2.1A and the first 6 participants, treated in Arm 2.1A, have met safety requirements, 13 participants with CRPC will start enrollment in Cohort 2A for treatment in Arm 2.2A (M7824 + BN-Brachyury + N-803).
• When 13 participants have enrolled in Arm 2.2A and the first 6 participants, treated in Arm 2.2A, have met safety requirements, 13 participants with CRPC will start enrollment in Cohort 2A for treatment in Arm 2.3A (M7824 + BN-Brachyury + N-803 + Epacadostat).

Phase IIB: expansion with randomized enrollment into Cohorts 2D and 2R, Arms 2.1B, 2.2B. and 2.3B

• Each Arm in Cohorts 2D and 2R: 2.1B, 2.2B and 2.3B will be open for additional enrollment (25 evaluable participants total) when the initial 13 participants have accrued, safety requirements are meet and a positive signal (defined as Objective Response by Response Evaluation Criteria in Solid Tumors (RECIST) 1.1 or sustained prostate-specific antigen (PSA) decrease >= 30% sustained for > 21 days) in >= 2 participants is shown.
• If only one arm is open for additional enrollment, participants will be directly assigned to this arm. If 2 arms are open for additional enrollment, participants will be randomized between these 2 open arms. If 3 arms are open for additional enrollment, participants will be randomized among these 3 open arms.

If there are >= 6 of 25 participants with a positive signal of activity in any expansion arm, that arm will be considered of interest for future studies.

• Study Type: Interventional
• Enrollment (Actual): 59
• Phase: Phase 2; Phase 1

Contacts and Locations

Study Locations

• United States
  • Maryland
    • Bethesda, Maryland, United States, 20892
      • National Institutes of Health Clinical Center

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 18 years and older (Adult, Older Adult)
• Accepts Healthy Volunteers: No

Description

• INCLUSION CRITERIA:

Participants must have histologically or cytologically confirmed any solid tumor (Cohort 1) or castration-resistant prostate cancer (CRPC), Cohorts 2A, 2D and 2R).

For the Cohort 1, eligible participants must have a histologically, cytologically or radiographically proven metastatic or locally advanced solid tumor of any type, for which there is no curative standard therapy or standard therapy has failed.

Castrate testosterone level (less than 50ng/dl or 1.7nmol/L). (Participants with a malignancy other than prostate cancer are excluded from this criterion).

Radiological confirmation of metastatic disease, or

Progressive disease at study entry defined as one or more of the following criteria occurring in the setting of castrate levels of testosterone:

--Radiographic progression defined as any new or enlarging bone lesions or growing lymph node disease, consistent with prostate cancer

OR

--prostate-specific antigen (PSA) progression defined by sequence of rising values separated by greater than 1 week (2 separate increasing values over a minimum of 1 ng/ml (Prostate Cancer Clinical Trials Working Group 3 (PCWG3) PSA eligibility criteria). If participants had been on flutamide, PSA progression is documented 4 weeks or more after withdrawal. For participants on bicalutamide or nilutamide disease progression is documented 6 or more weeks after withdrawal. The requirement for a 4-6 week withdrawal period following discontinuation of flutamide, nilutamide or bicalutamide only applies to participants who have been on these drugs for at least the prior 6 months. For all other participants they must stop bicalutamide, nilutamide or flutamide the day prior to enrollment.

Asymptomatic or mildly symptomatic form prostate cancer; no use of regularly scheduled opiate analgesics for prostate cancer-related pain. (Participants with a malignancy other than prostate cancer are excluded from this criterion).

Participants must agree to continuation of androgen deprivation therapy (ADT) with a gonadotropin-releasing hormone analogue/antagonist or bilateral orchiectomy. (Participants with a malignancy other than prostate cancer are excluded from this criterion). Participants may also continue oral androgen receptor antagonist/anti-androgen therapy (e.g. enzalutamide or abiraterone) unless enrolling to Arm 2.3A or 2.3B due to concerns regarding Cytochrome P450 (CYP)mediated drug-drug interactions epacadostat.

Participants must have had the following prior therapy:

Testosterone lowering therapy for castration-resistant prostate cancer (CRPC)

In addition to continuation of androgen deprivation therapy (ADT) (unless status post bilateral orchiectomy) eligible patients must have received and had PSA or radiographic progression on enzalutamide (or other oral androgen receptor antagonist e.g. darolutamide or apalutamdide) or abiraterone acetate.

Participants who have tumors known to be microsatellite instability high/mismatch repair deficient or tumor mutational burden high must have received prior pembrolizumab.

Participants with known pathogenic homologous recombination repair mutations for which there is evidence of benefit with poly (adenosine diphosphate-ribose) polymerase (PARP) inhibitors (e.g. BReast CAncer gene 1 (BRCA1). BReast CAncer gene 2 (BRCA2), ataxia-telangiectasia mutated (ATM) must have received prior PARP inhibitor.

Age greater than or equal to 18 years.

Eastern Cooperative Oncology Group (ECOG) performance status less than or equal to 1

Participants must have adequate organ and marrow function as defined below:

• Absolute neutrophil count greater than or equal to 1000/mcL
• Platelets greater than or equal to 100,000/mcL
• Hemoglobin greater than or equal to 9.0 g/dL
• Total bilirubin within normal institutional limits; in participants with Gilbert's, less than or equal to 3.0 mg/dL
• Aspartate aminotransferase (AST) serum glutamic-oxaloacetic transaminase (SGOT)/alanine aminotransferase (ALT) serum glutamic-pyruvic transaminase (SGPT) less than or equal to 2.5X upper limit of normal. For subjects with liver involvement in their tumor, AST less than or equal to 3.5. X upper limit of normal (ULN), ALT less than or equal to 3.5 X ULN, and bilirubin less than or equal to 3.0 is acceptable
• Creatinine within 1.5X upper limit of normal institutional limits

The effects of Bavarian Nordic (BN)-Brachyury, bintrafusp alfa (M7824), Anktiva (N-803), and Epacadostat on the developing human fetus are unknown. For this reason, men and women must agree to use adequate contraception (hormonal or barrier method of birth control; abstinence) prior to study entry, during the study and maintain such contraception until 4 months following the last dose of any study agent. Should a woman become pregnant or suspect she is pregnant while she or her partner is participating in this study, she should inform her partner s treating physician immediately.

Ability of subject to understand and the willingness to sign a written informed consent document.

Participants with successfully treated hepatitis C virus (HCV) are eligible if HCV viral load is undetectable.

EXCLUSION CRITERIA:

Participants who are immunocompromised as follows:

• Human immunodeficiency virus positivity due to the potential for decreased tolerance, and potential to be at risk for severe side effects with immunotherapies. These concerns are relevant to all drugs, as drug-drug interactions among antiretrovirals and immunotherapies are yet uncharacterized.
• Chronic administration (defined as daily or every other day for continued use greater than 14 days) of systemic corticosteroids or other immune suppressive drugs, within 28 days before treatment on study. Nasal, or inhaled steroid, topical steroid creams and eye drops for small body areas are allowed. Physiologic doses of steroids are permitted, e.g., a participant taking hydrocortisone for adrenal insufficiency or a patient recently on abiraterone (administered with 10 mg of prednisone daily) who is tapering off of prednisone is allowed to continue that taper.

• Participants who have undergone allogeneic peripheral stem cell transplantation, or solid organ transplantation requiring immunosuppression

  • Active autoimmune disease, except participants with type 1 diabetes mellitus, vitiligo, psoriasis, hypo- or hyperthyroid disease not requiring current immunosuppression, or with other endocrine disorders on replacement hormones or are not excluded if the condition is well controlled.

Prostate cancer participants with a history of brain/leptomeningeal metastasis, since these participants have a very poor prognosis, and immunotherapy may take time to lead to beneficial clinical effects. Participants with brain or CNS metastases enrolling to arm 1.1 are eligible if they are status post definitive radiotherapy or surgery, and are asymptomatic

History of allergic reactions attributed to compounds of similar chemical or biologic composition to study agents to be used in the cohort the subject will be enrolled into.

Known allergy to eggs, egg products, aminoglycoside antibiotics (for example, gentamicin or tobramycin).

Any condition which, in the opinion of the investigator, would prevent full participation in this trial (including the long-term follow-up), or would interfere with the evaluation of the trial endpoints.

Participants with prior investigational drug, chemotherapy, immunotherapy or any prior radiotherapy (except for palliative bone directed therapy) within the past 28 days prior to enrollment, except if the investigator has assessed that all residual treatment-related toxicities have resolved or are minimal and feel the participant is otherwise suitable for enrollment.

Uncontrolled intercurrent acute or chronic illness including, but not limited to, ongoing or active infection, symptomatic congestive heart failure (>New York Heart Association Class I), hepatic disease, unstable angina pectoris, serious cardiac arrhythmia, requiring medication, uncontrolled hypertension (Systolic blood pressure (SBP) >170/ diastolic blood pressure (DBP)>105) or psychiatric illness/social situations within 12 months that would limit compliance with study requirements.

Use of herbal products that may decrease PSA levels (e.g., saw palmetto)

Participants who have had cytotoxic chemotherapy for metastatic castration-resistant prostate cancer within the past 3 months. (Participants who have had docetaxel for metastatic castration sensitive per Chemohormonal Therapy Versus Androgen Ablation Randomized Trial for Extensive Disease in Prostate Cancer (CHAARTED) data may enroll as long as they did not have progressive disease while on docetaxel and are 3 months removed from treatment, with all treatment related toxicities resolving to at least grade 1.)

Participants who have undergone major surgery within 4 weeks of enrollment. A biopsy will not preclude a participant from starting study.

Participants with a history of hepatitis B (HBV) are excluded due to potential risk for viral reactivation and resulting liver injury in persons with latent HBV

Subjects unwilling to accept blood products or blood transfusions as medically indicated. As there is a risk of severe bleeding with M7824, participants must be willing to receive blood transfusions if medically necessary for their own safety

For participants enrolling in Arm 2.3A and for participants who may be randomized to Arm 2.3B, the following additional exclusion criteria will apply:

• Subjects receiving Monoamine Oxidase Inhibitors (MAOIs) or a drug which has significant MAOI activity (meperidine, linezolid, methylene blue) within the 21 days before initiation of study therapy are excluded.
• Since epacadostats metabolism may be altered by drugs that inhibit uridine diphosphate (UDP)-glucuronosyltransferase, UGT1A9 (see Appendix D), Participants receiving such drugs within 21 days of initiation of study therapy are excluded.
• Participants receiving agents that are substrates of Cytochrome P450 1A2 (CYP1A2), cytochrome P450 2C8 (CYP2C8), and cytochrome P450 2C19 (CYP2C19) or affected by organic anion transporting polypeptide 1B1 (OATP1B1) or organic anion transporting polypeptide 1B3 (OATP1B3) within 21 days of initiation of study therapy or 5 half-lives of the agent (whichever is shorter) are excluded. Participants receiving medications that are substrates of these enzymes/transporters but are not listed in the appendix or participants receiving substrates of CYP2B6 and CYP3A will be evaluated on a case-by-case basis prior to enrollment. Participants who consume caffeine are eligible but must agree to moderate consumption.
• Subjects receiving coumarin-based anticoagulants (e. Coumadin) are excluded.

• Subjects having any history of Serotonin Syndrome (SS) after receiving serotonergic drugs are excluded.

  --Participants with a QTc corrected for heart rate by Fridericia's formula (QTcF) interval > 480 milliseconds at the screening are excluded. In the event that a single QTcF is > 480 milliseconds, the subject may enroll if the average QTcF for the 3 electrocardiogram (ECG's) is < 480 milliseconds. For subjects with an intraventricular conduction delay (QRS interval > 120 milliseconds), the corrected JT interval, (JTc) interval may be used in place of the QTc. The JTc must be < 340 milliseconds if JTc is used in place of the QTc. QTc prolongation due to pacemaker may enroll if the JTc is normal.

• Subjects with left bundle branch block are excluded.
• Pregnant women are excluded from this study because investigational agents used in this study (BN-Brachyury, M7824, N-803, and/or Epacadostat) could have teratogenic or abortifacient effects. Because there is an unknown but potential risk for adverse events in nursing infants secondary to treatment of the mother with these investigational agents, breastfeeding should be discontinued if the mother is treated with either of them.

Study Plan

How is the study designed?

Design Details

• Primary Purpose: Treatment
• Allocation: Randomized
• Interventional Model: Sequential Assignment
• Masking: None (Open Label)

Number of Arms

7

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
Experimental: Arm 1.1 Bintrafusp alfa (M7824) + Anktiva (N-803); Bintrafusp alfa (M7824) + Anktiva (N-803)	Diagnostic test: MRI; If clinically indicated.; Other Names: Magnetic resonance imaging; Biological: M7824; 1,200 mg intravenous (IV) once every 2 weeks.; Other Names: Bintrafusp alfa; Drug: N-803; 8-15 mcg/kg subcutaneous every 2 weeks.; Other Names: Anktiva; Drug: Acetaminophen; Antipyretic medication, orally, prior to infusion, up to 650mg.; Other Names: Tylenol; Ofirmev; FeverAll; Drug: Ibuprofen; Antipyretic medication, orally, prior to infusion, up to 600mg.; Other Names: Advil; Advil Migraine; Drug: Naproxen; Antipyretic medication, orally, prior to infusion, up to 500mg.; Other Names: Aleve; Naprosyn; Anaprox DS; Other: Benadryl; 50mg orally prior to infusion.; Other Names: Diphenhydramine; Diagnostic test: EKG; Baseline and principal investigator discretion.; Other Names: Electrocardiogram; Diagnostic test: Bone scan; Restaging every 12 weeks.; Other Names: Bone scintigraphy; Diagnostic test: CT chest, abdomen and pelvis; Every 12 weeks after start of therapy.; Other Names: Computed tomography chest, abdomen and pelvis
Experimental: Arm 2.1A Bintrafusp alfa (M7824) + Bavarian Nordic (BN)-Brachyury during phase IIA; Bintrafusp alfa (M7824) + Bavarian Nordic (BN)-Brachyury during phase IIA	Biological: MVA-BN-Brachyury; MVA-BN-Brachyury will be administered subcutaneously (2 doses 2 weeks apart).; Other Names: modified vaccinia Ankara Bavarian Nordic Brachyury; Diagnostic test: MRI; If clinically indicated.; Other Names: Magnetic resonance imaging; Biological: M7824; 1,200 mg intravenous (IV) once every 2 weeks.; Other Names: Bintrafusp alfa; Drug: Acetaminophen; Antipyretic medication, orally, prior to infusion, up to 650mg.; Other Names: Tylenol; Ofirmev; FeverAll; Drug: Ibuprofen; Antipyretic medication, orally, prior to infusion, up to 600mg.; Other Names: Advil; Advil Migraine; Drug: Naproxen; Antipyretic medication, orally, prior to infusion, up to 500mg.; Other Names: Aleve; Naprosyn; Anaprox DS; Other: Benadryl; 50mg orally prior to infusion.; Other Names: Diphenhydramine; Diagnostic test: EKG; Baseline and principal investigator discretion.; Other Names: Electrocardiogram; Diagnostic test: Bone scan; Restaging every 12 weeks.; Other Names: Bone scintigraphy; Diagnostic test: CT chest, abdomen and pelvis; Every 12 weeks after start of therapy.; Other Names: Computed tomography chest, abdomen and pelvis; Biological: FPV-Brachyury; FPV-Brachyury will be given 2 weeks after second dose of modified vaccinia Ankara (MVA)-Bavarian Nordic (BN)-Brachyury, then every 4 weeks until 6 months, then every 3 months for 2 years, then every 6 months.; Other Names: Fowlpox Virus Brachyury
Experimental: Arm 2.1B Bintrafusp alfa (M7824) + Bavarian Nordic (BN)-Brachyury during phase IIB; Bintrafusp alfa (M7824) + Bavarian Nordic (BN)-Brachyury during phase IIB	Biological: MVA-BN-Brachyury; MVA-BN-Brachyury will be administered subcutaneously (2 doses 2 weeks apart).; Other Names: modified vaccinia Ankara Bavarian Nordic Brachyury; Diagnostic test: MRI; If clinically indicated.; Other Names: Magnetic resonance imaging; Biological: M7824; 1,200 mg intravenous (IV) once every 2 weeks.; Other Names: Bintrafusp alfa; Drug: Acetaminophen; Antipyretic medication, orally, prior to infusion, up to 650mg.; Other Names: Tylenol; Ofirmev; FeverAll; Drug: Ibuprofen; Antipyretic medication, orally, prior to infusion, up to 600mg.; Other Names: Advil; Advil Migraine; Drug: Naproxen; Antipyretic medication, orally, prior to infusion, up to 500mg.; Other Names: Aleve; Naprosyn; Anaprox DS; Other: Benadryl; 50mg orally prior to infusion.; Other Names: Diphenhydramine; Diagnostic test: EKG; Baseline and principal investigator discretion.; Other Names: Electrocardiogram; Diagnostic test: Bone scan; Restaging every 12 weeks.; Other Names: Bone scintigraphy; Diagnostic test: CT chest, abdomen and pelvis; Every 12 weeks after start of therapy.; Other Names: Computed tomography chest, abdomen and pelvis; Biological: FPV-Brachyury; FPV-Brachyury will be given 2 weeks after second dose of modified vaccinia Ankara (MVA)-Bavarian Nordic (BN)-Brachyury, then every 4 weeks until 6 months, then every 3 months for 2 years, then every 6 months.; Other Names: Fowlpox Virus Brachyury
Experimental: Arm 2.2A Bintrafusp alfa (M7824) + Bavarian Nordic (BN)-Brachyury + Anktiva (N-803) during phase IIA; Bintrafusp alfa (M7824) + Bavarian Nordic (BN)-Brachyury + Anktiva (N-803) during phase IIA	Biological: MVA-BN-Brachyury; MVA-BN-Brachyury will be administered subcutaneously (2 doses 2 weeks apart).; Other Names: modified vaccinia Ankara Bavarian Nordic Brachyury; Diagnostic test: MRI; If clinically indicated.; Other Names: Magnetic resonance imaging; Biological: M7824; 1,200 mg intravenous (IV) once every 2 weeks.; Other Names: Bintrafusp alfa; Drug: N-803; 8-15 mcg/kg subcutaneous every 2 weeks.; Other Names: Anktiva; Drug: Acetaminophen; Antipyretic medication, orally, prior to infusion, up to 650mg.; Other Names: Tylenol; Ofirmev; FeverAll; Drug: Ibuprofen; Antipyretic medication, orally, prior to infusion, up to 600mg.; Other Names: Advil; Advil Migraine; Drug: Naproxen; Antipyretic medication, orally, prior to infusion, up to 500mg.; Other Names: Aleve; Naprosyn; Anaprox DS; Other: Benadryl; 50mg orally prior to infusion.; Other Names: Diphenhydramine; Diagnostic test: EKG; Baseline and principal investigator discretion.; Other Names: Electrocardiogram; Diagnostic test: Bone scan; Restaging every 12 weeks.; Other Names: Bone scintigraphy; Diagnostic test: CT chest, abdomen and pelvis; Every 12 weeks after start of therapy.; Other Names: Computed tomography chest, abdomen and pelvis; Biological: FPV-Brachyury; FPV-Brachyury will be given 2 weeks after second dose of modified vaccinia Ankara (MVA)-Bavarian Nordic (BN)-Brachyury, then every 4 weeks until 6 months, then every 3 months for 2 years, then every 6 months.; Other Names: Fowlpox Virus Brachyury
Experimental: Arm 2.2B Bintrafusp alfa (M7824) + Bavarian Nordic (BN)-Brachyury + Anktiva (N-803) during phase IIB; Bintrafusp alfa (M7824) + Bavarian Nordic (BN)-Brachyury + Anktiva (N-803) during phase IIB	Biological: MVA-BN-Brachyury; MVA-BN-Brachyury will be administered subcutaneously (2 doses 2 weeks apart).; Other Names: modified vaccinia Ankara Bavarian Nordic Brachyury; Diagnostic test: MRI; If clinically indicated.; Other Names: Magnetic resonance imaging; Biological: M7824; 1,200 mg intravenous (IV) once every 2 weeks.; Other Names: Bintrafusp alfa; Drug: N-803; 8-15 mcg/kg subcutaneous every 2 weeks.; Other Names: Anktiva; Drug: Acetaminophen; Antipyretic medication, orally, prior to infusion, up to 650mg.; Other Names: Tylenol; Ofirmev; FeverAll; Drug: Ibuprofen; Antipyretic medication, orally, prior to infusion, up to 600mg.; Other Names: Advil; Advil Migraine; Drug: Naproxen; Antipyretic medication, orally, prior to infusion, up to 500mg.; Other Names: Aleve; Naprosyn; Anaprox DS; Other: Benadryl; 50mg orally prior to infusion.; Other Names: Diphenhydramine; Diagnostic test: EKG; Baseline and principal investigator discretion.; Other Names: Electrocardiogram; Diagnostic test: Bone scan; Restaging every 12 weeks.; Other Names: Bone scintigraphy; Diagnostic test: CT chest, abdomen and pelvis; Every 12 weeks after start of therapy.; Other Names: Computed tomography chest, abdomen and pelvis; Biological: FPV-Brachyury; FPV-Brachyury will be given 2 weeks after second dose of modified vaccinia Ankara (MVA)-Bavarian Nordic (BN)-Brachyury, then every 4 weeks until 6 months, then every 3 months for 2 years, then every 6 months.; Other Names: Fowlpox Virus Brachyury
Experimental: Arm 2.3A M7824 + BN-Brachyury + Anktiva (N-803) + Epacadostat during phase IIA; Bintrafusp alfa (M7824) + Bavarian Nordic (BN)-Brachyury + Anktiva (N-803) + Epacadostat during phase IIA	Biological: MVA-BN-Brachyury; MVA-BN-Brachyury will be administered subcutaneously (2 doses 2 weeks apart).; Other Names: modified vaccinia Ankara Bavarian Nordic Brachyury; Diagnostic test: MRI; If clinically indicated.; Other Names: Magnetic resonance imaging; Biological: M7824; 1,200 mg intravenous (IV) once every 2 weeks.; Other Names: Bintrafusp alfa; Drug: N-803; 8-15 mcg/kg subcutaneous every 2 weeks.; Other Names: Anktiva; Drug: Acetaminophen; Antipyretic medication, orally, prior to infusion, up to 650mg.; Other Names: Tylenol; Ofirmev; FeverAll; Drug: Ibuprofen; Antipyretic medication, orally, prior to infusion, up to 600mg.; Other Names: Advil; Advil Migraine; Drug: Naproxen; Antipyretic medication, orally, prior to infusion, up to 500mg.; Other Names: Aleve; Naprosyn; Anaprox DS; Other: Benadryl; 50mg orally prior to infusion.; Other Names: Diphenhydramine; Diagnostic test: EKG; Baseline and principal investigator discretion.; Other Names: Electrocardiogram; Diagnostic test: Bone scan; Restaging every 12 weeks.; Other Names: Bone scintigraphy; Diagnostic test: CT chest, abdomen and pelvis; Every 12 weeks after start of therapy.; Other Names: Computed tomography chest, abdomen and pelvis; Biological: FPV-Brachyury; FPV-Brachyury will be given 2 weeks after second dose of modified vaccinia Ankara (MVA)-Bavarian Nordic (BN)-Brachyury, then every 4 weeks until 6 months, then every 3 months for 2 years, then every 6 months.; Other Names: Fowlpox Virus Brachyury; Drug: Epacadostat; 600 mg orally twice daily (1200 MG total).; Other Names: IDO1
Experimental: Arm 2.3B M7824 + BN-Brachyury + Anktiva (N-803) + Epacadostat during phase IIB; Bintrafusp alfa (M7824) + Bavarian Nordic (BN)-Brachyury + Anktiva (N-803) + Epacadostat during phase IIB	Biological: MVA-BN-Brachyury; MVA-BN-Brachyury will be administered subcutaneously (2 doses 2 weeks apart).; Other Names: modified vaccinia Ankara Bavarian Nordic Brachyury; Diagnostic test: MRI; If clinically indicated.; Other Names: Magnetic resonance imaging; Biological: M7824; 1,200 mg intravenous (IV) once every 2 weeks.; Other Names: Bintrafusp alfa; Drug: N-803; 8-15 mcg/kg subcutaneous every 2 weeks.; Other Names: Anktiva; Drug: Acetaminophen; Antipyretic medication, orally, prior to infusion, up to 650mg.; Other Names: Tylenol; Ofirmev; FeverAll; Drug: Ibuprofen; Antipyretic medication, orally, prior to infusion, up to 600mg.; Other Names: Advil; Advil Migraine; Drug: Naproxen; Antipyretic medication, orally, prior to infusion, up to 500mg.; Other Names: Aleve; Naprosyn; Anaprox DS; Other: Benadryl; 50mg orally prior to infusion.; Other Names: Diphenhydramine; Diagnostic test: EKG; Baseline and principal investigator discretion.; Other Names: Electrocardiogram; Diagnostic test: Bone scan; Restaging every 12 weeks.; Other Names: Bone scintigraphy; Diagnostic test: CT chest, abdomen and pelvis; Every 12 weeks after start of therapy.; Other Names: Computed tomography chest, abdomen and pelvis; Biological: FPV-Brachyury; FPV-Brachyury will be given 2 weeks after second dose of modified vaccinia Ankara (MVA)-Bavarian Nordic (BN)-Brachyury, then every 4 weeks until 6 months, then every 3 months for 2 years, then every 6 months.; Other Names: Fowlpox Virus Brachyury; Drug: Epacadostat; 600 mg orally twice daily (1200 MG total).; Other Names: IDO1

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Clinical Benefit With Any of a Set of 3 Possible Treatments for Participants With Metastatic Castration-resistant Prostate Cancer (mCRPC) Reported With an 80% Confidence Interval	Objective clinical response and/or prostate-specific antigen (PSA) decline of >=30% sustained for a minimum of 21 days. Any of these is considered 'clinical benefit.' Objective response was assessed by the response evaluation criteria in solid tumors (RECIST) 1.1. Complete response (CR) is disappearance of all non-target lesions and normalization of tumor marker level. Partial response (PR) is at least a 30% decrease in the sum of the diameters of target lesions. Stable disease (SD) is neither sufficient shrinkage to qualify for PR nor sufficient increase to qualify for PD. Progressive disease (PD) is at least a 20% increase in the sum of the diameters of target lesions. Three treatments evaluated are: Phase II Bavarian Nordic (BN) Brachyury + Bintrafusp alfa (M7824), Phase II Bavarian Nordic (BN) Brachyury + Bintrafusp alfa (M7824) + Anktiva (N-803), and Phase II Bavarian Nordic (BN) Brachyury + Bintrafusp alfa (M7824) + Anktiva (N-803) + Epacadostat.	From enrollment up to 49 weeks
Clinical Benefit(Objective Response if Measurable Disease by Response Evaluation Criteria in Solid Tumors v1.1)With Any of 3 Possible Treatments for Participants With Metastatic Castration-resistant Prostate Cancer Reported With a 95% Confidence Interval	Objective response was assessed by the response evaluation criteria in solid tumors (RECIST) 1.1. Complete response (CR) is disappearance of all non-target lesions and normalization of tumor marker level. Partial response (PR) is at least a 30% decrease in the sum of the diameters of target lesions. Stable disease (SD) is neither sufficient shrinkage to qualify for PR nor sufficient increase to qualify for PD. Progressive disease (PD) is at least a 20% increase in the sum of the diameters of target lesions. Three treatments evaluated are: Phase II Bavarian Nordic (BN) Brachyury + Bintrafusp alfa (M7824), Phase II Bavarian Nordic (BN) Brachyury + Bintrafusp alfa (M7824) + Anktiva (N-803), and Phase II Bavarian Nordic (BN) Brachyury + Bintrafusp alfa (M7824) + Anktiva (N-803) + Epacadostat.	From enrollment up to 49 weeks
Phase I/II: Number of Participants With Grades 3, 4, and/or 5 Serious and/or Non-serious Dose-limiting Toxicities (DLT)	A DLT is defined as any of the following adverse events (AE) possibly related to study drugs that occur within 21 days of the start of treatment. Adverse events were assessed by the Common Terminology Criteria for Adverse Events (CTCAE) v5.0. Grade 3 is moderate. Grade 4 is life-threatening, and Grade 5 is death related to AE. A DLT is any grade ≥ 4 hematologic toxicity or grade 3 thrombocytopenia with associated bleeding; any grade ≥ 3 non-hematologic toxicity, except for any of the following: transient (≤ 48 hour) grade 3 fatigue, local reactions, flu-like symptoms, fever, headache, or nausea, emesis, and diarrhea; or CTCAE Grade 3 skin toxicity lasting less than five days. A non-serious AE is any untoward medical occurrence. A serious AE is an adverse event or suspected adverse reaction that results in death, a life-threatening adverse drug experience, hospitalization, disruption of the ability to conduct normal life functions, or congenital anomaly/birth defect.	Within 21 days of the start of treatment

Secondary Outcome Measures

Outcome Measure	Measure Description	Time Frame
6-month Progression Free Survival (PFS) Probability	6-month progression free survival was measured from the on-study date until progression or death without progression in participants and reported with a 95% confidence interval. Progression was assessed by the Response Evaluation Criteria in Solid Tumors (RECIST) v1.1 and is defined as at least a 20% increase in the sum of the diameters of target lesions, taking as reference the smallest sum on study (this includes the baseline sum if that is the smallest on study). The appearance of one or more new lesions is also considered progressions.	6-months
Number of Participants With Grades 1, 2, 3, and 4 Non-serious Adverse Events Related to Treatment Treated With Combinations and Bintrafusp Alfa (M7824) + Anktiva (N-803)	Number of participants with grades 1, 2, 3, and 4 non-serious adverse events related to treatment treated with combinations and Bintrafusp alfa (M7824) + Anktiva (N-803).	3 weeks dose-limiting toxicity (DLT) period
Number of Participants With Grades 3, 4, and/or 5 Serious Adverse Events Related to Treatment Treated With Combinations and Bintrafusp Alfa (M7824) + Anktiva (N-803)	Number of participants with grades 3 4, and/or 5 serious adverse events related to treatment treated with combinations and Bintrafusp alfa (M7824) + Anktiva (N-803).	3 weeks dose-limiting toxicity (DLT) period

Other Outcome Measures

Outcome Measure	Measure Description	Time Frame
Number of Participants With Serious and/or Non-serious Adverse Events Assessed by the Common Terminology Criteria for Adverse Events (CTCAE v5.0)	Here is the number of participants with serious and/or non-serious adverse events assessed by the Common Terminology Criteria for Adverse Events (CTCAE v5.0). A non-serious adverse event is any untoward medical occurrence. A serious adverse event is an adverse event or suspected adverse reaction that results in death, a life-threatening adverse drug experience, hospitalization, disruption of the ability to conduct normal life functions, congenital anomaly/birth defect or important medical events that jeopardize the patient or subject and may require medical or surgical intervention to prevent one of the previous outcomes mentioned.	Adverse Events were monitored/assessed from the first study intervention, up to 49 weeks.

Collaborators and Investigators

• Sponsor: National Cancer Institute (NCI)
• Investigators: Principal Investigator: James L Gulley, M.D., National Cancer Institute (NCI)

Publications and helpful links

General Publications

• Fabian KP, Padget MR, Fujii R, Schlom J, Hodge JW. Differential combination immunotherapy requirements for inflamed (warm) tumors versus T cell excluded (cool) tumors: engage, expand, enable, and evolve. J Immunother Cancer. 2021 Feb;9(2):e001691. doi: 10.1136/jitc-2020-001691.
• Redman JM, Steinberg SM, Gulley JL. Quick efficacy seeking trial (QuEST1): a novel combination immunotherapy study designed for rapid clinical signal assessment metastatic castration-resistant prostate cancer. J Immunother Cancer. 2018 Sep 18;6(1):91. doi: 10.1186/s40425-018-0409-8.

Helpful Links

• NIH Clinical Center Detailed Web Page

Study record dates

Study Major Dates

• Study Start (Actual): May 1, 2018
• Primary Completion (Actual): October 8, 2024
• Study Completion (Actual): October 8, 2024

Study Registration Dates

• First Submitted: April 10, 2018
• First Submitted That Met QC Criteria: April 10, 2018
• First Posted (Actual): April 11, 2018

Study Record Updates

• Last Update Posted (Estimated): September 30, 2025
• Last Update Submitted That Met QC Criteria: September 11, 2025
• Last Verified: September 1, 2025

More Information

Terms related to this study

• Keywords: Immunotherapy; Combined Treatment; PD-1/PD-L1; Tumor-Specific T Cells; Novel Cancer Vaccine
• Additional Relevant MeSH Terms: Urogenital Diseases; Genital Diseases; Genital Neoplasms, Male; Urogenital Neoplasms; Neoplasms by Site; Neoplasms; Genital Diseases, Male; Prostatic Diseases; Male Urogenital Diseases; Prostatic Neoplasms; Organic Chemicals; Diagnostic Techniques and Procedures; Diagnosis; Hydrocarbons; Hydrocarbons, Cyclic; Carboxylic Acids; Naphthalenes; Polycyclic Aromatic Hydrocarbons; Hydrocarbons, Aromatic; Polycyclic Compounds; Anilides; Amides; Aniline Compounds; Amines; Acetanilides; Benzene Derivatives; Tomography; Diagnostic Imaging; Ethylamines; Diagnostic Techniques, Cardiovascular; Acids, Carbocyclic; Heart Function Tests; Electrodiagnosis; Phenylpropionates; Naphthaleneacetic Acids; Benzhydryl Compounds; Acetaminophen; Naproxen; Diphenhydramine; Ibuprofen; Magnetic Resonance Imaging; ALT-803; Electrocardiography; bintrafusp alfa protein, human; epacadostat
• Other Study ID Numbers: 180078; 18-C-0078

Plan for Individual participant data (IPD)

• Plan to Share Individual Participant Data (IPD)?: YES
• IPD Plan Description: All individual participant data (IPD) recorded in the medical record will be shared with intramural investigators upon request. In addition, all large-scale genomic sequencing data will be shared with subscribers to the database of Genotypes and Phenotypes (dbGaP).
• IPD Sharing Time Frame: Clinical data available during the study and indefinitely. Genomic data are available once genomic data are uploaded per protocol Genomic Data Sharing (GDS) plan for as long as database is active.
• IPD Sharing Access Criteria: Clinical data will be made available via subscription to Biomedical Translational Research Information System (BTRIS) and with the permission of the study principal investigator (PI). Genomic data are made available via the database of Genotypes and Phenotypes (dbGaP) through requests to the data custodians.
• IPD Sharing Supporting Information Type: STUDY_PROTOCOL; SAP; ICF

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: Yes
• Studies a U.S. FDA-regulated device product: No