- ICH GCP
- US Clinical Trials Registry
- Clinical Trial NCT04214184
Biomarkers of Increased Free Living Sleep Time
November 29, 2023 updated by: Christopher Depner, University of Utah
Biomarkers and Altered Metabolic Pathways During Sleep Loss
This protocol will increase sleep duration in participants who maintain less than 6 hours sleep per night, to target the recommended 7 hours of sleep per night.
The focus of this study is determine how increasing nightly sleep duration in these individuals who maintain less than 6 hours sleep per night changes their plasma metabolome and insulin sensitivity.
The primary outcome will examine changes in branched-chain amino acids and the secondary outcome will examine changes in insulin sensitivity.
The investigators will also determine if changes in plasma metabolites can be used as a biomarker to discriminate between adequate versus insufficient sleep.
Study Overview
Status
Recruiting
Intervention / Treatment
Detailed Description
Impaired sleep affects millions of people each year representing an important public health issue.
This project will utilize metabolomics approaches to identify potential mechanisms underlying increased cardiometabolic risk associated with insufficient sleep and to identify potential biomarkers in the blood that respond to insufficient sleep.
Investigators will conduct a controlled in-laboratory insufficient protocol where participants will sleep in the lab for one night with sleep timing based on their habitual insufficient sleep schedule.
In the morning, plasma will be collected for metabolomics analyses and participants will complete an oral glucose tolerance test for insulin sensitivity analyses.
Participants will then complete a 4 -week increased sleep duration intervention targeting the recommended 7 hours of sleep per night.
Following this intervention participants will again sleep in the lab for one night on their new sleep schedule.
In the morning, plasma will be collected for metabolomics analyses and participants will complete an oral glucose tolerance test for insulin sensitivity analyses.
Investigators anticipate these findings will be the first step in developing biomarkers of impaired sleep under free-living sleep conditions, and to determine how such biomarkers relate to insulin sensitivity changes associated with sleep loss.
Study Type
Interventional
Enrollment (Estimated)
46
Phase
- Not Applicable
Contacts and Locations
This section provides the contact details for those conducting the study, and information on where this study is being conducted.
Study Contact
- Name: Christopher Depner
- Phone Number: 303-735-1923
- Email: christopher.depner@utah.edu
Study Locations
-
-
Utah
-
Salt Lake City, Utah, United States, 84108
- Recruiting
- Sleep Wake Center--University of Utah
-
Contact:
- Christopher Depner
- Phone Number: 801-581-2275
- Email: sleepstudyparticipant@utah.edu
-
-
Participation Criteria
Researchers look for people who fit a certain description, called eligibility criteria. Some examples of these criteria are a person's general health condition or prior treatments.
Eligibility Criteria
Ages Eligible for Study
18 years to 35 years (Adult)
Accepts Healthy Volunteers
Yes
Description
Inclusion Criteria:
18-35 years old; men and women
a. Equal numbers of women and men will be included.
- Body Mass Index (BMI) of > 18.5 and <24.9.
- Inactive to habitual moderate physical activity level (<5 days of exercise per week).
- Sleep/wake history: habitual sleep duration less than 6 hours per night.
- Altitude history: Potential participants must have lived at Denver altitude or higher for at least 3 months.
Exclusion Criteria:
- Any clinically significant unstable medical or surgical condition within the last year (treated or untreated).
- Any clinically significant psychiatric condition, as defined by DSM-IV-TR. I
- Any clinically significant sleep disorder.
- Use of prescription medications/supplements within one month or need of these medications at any time during the study.
- Symptoms of active illness (e.g., fever).
- Uncorrected visual impairment
- History of shift work in prior year or travel more than one time zone in three weeks prior to study.
- Participants must be entirely drug-free of illicit drugs, medications, nicotine and herbal products for one month prior to study.
- Blood donation in the 30 days prior to inpatient study.
- Ovulating women will be selected on the basis of a history of regular menstrual cycle ranging in length from 25-32 days with a maximum of three days variation month-to-month. They will have no history of prior gynecological pathology, be at least 1 year post-partum, not breast-feeding and not pregnant (HCG pregnancy test at screening and upon admission to the inpatient protocol).
Study Plan
This section provides details of the study plan, including how the study is designed and what the study is measuring.
How is the study designed?
Design Details
- Primary Purpose: Basic Science
- Allocation: N/A
- Interventional Model: Single Group Assignment
- Masking: None (Open Label)
Arms and Interventions
Participant Group / Arm |
Intervention / Treatment |
---|---|
Experimental: Increased Sleep Duration Intervention
Following baseline assessments, participants will complete a 4-week increased sleep duration intervention followed by one night of sleep in the lab on this increased sleep duration schedule.
In the morning, participants will have blood collected for metabolomics analyses and complete oral glucose tolerance testing
|
Participants will increase their nightly time in bed by 2 hours per night for 4 weeks to target the recommended 7 hours of sleep per night.
|
What is the study measuring?
Primary Outcome Measures
Outcome Measure |
Measure Description |
Time Frame |
---|---|---|
Metabolomics-branched chain amino acids change from baseline
Time Frame: Morning fasted blood will be collected for analyses at baseline and after the four week increased sleep duration intervention.
|
Investigators will measure the abundance of the branched chain amino acids (valine, leucine, isoleucine) in plasma at baseline and at post intervention.
|
Morning fasted blood will be collected for analyses at baseline and after the four week increased sleep duration intervention.
|
Insulin Sensitivity change from baseline
Time Frame: Oral glucose tolerance testing will take about 3 hours to complete, and will be tested at baseline and after the four week increased sleep duration intervention.
|
Investigators will measure insulin sensitivity using the oral glucose tolerance test in the morning after overnight sleep assessments in the lab, at baseline and after the four week increased sleep duration intervention.
|
Oral glucose tolerance testing will take about 3 hours to complete, and will be tested at baseline and after the four week increased sleep duration intervention.
|
Secondary Outcome Measures
Outcome Measure |
Measure Description |
Time Frame |
---|---|---|
Untargeted Metabolomics change from baseline
Time Frame: Morning fasted blood will be collected for analyses at baseline and after the four week increased sleep duration intervention.
|
Investigators can detect relative abundance of ~4,000 plasma metabolites.
A combination of metabolites that can discriminate between adequate versus insufficient sleep will be identified as a potential biomarkers of insufficient sleep in free-living adults.
|
Morning fasted blood will be collected for analyses at baseline and after the four week increased sleep duration intervention.
|
Collaborators and Investigators
This is where you will find people and organizations involved with this study.
Sponsor
Collaborators
Investigators
- Principal Investigator: Christopher Depner, University of Utah
Study record dates
These dates track the progress of study record and summary results submissions to ClinicalTrials.gov. Study records and reported results are reviewed by the National Library of Medicine (NLM) to make sure they meet specific quality control standards before being posted on the public website.
Study Major Dates
Study Start (Actual)
December 2, 2019
Primary Completion (Estimated)
June 30, 2024
Study Completion (Estimated)
July 31, 2024
Study Registration Dates
First Submitted
December 17, 2019
First Submitted That Met QC Criteria
December 26, 2019
First Posted (Actual)
January 2, 2020
Study Record Updates
Last Update Posted (Actual)
December 1, 2023
Last Update Submitted That Met QC Criteria
November 29, 2023
Last Verified
November 1, 2023
More Information
Terms related to this study
Additional Relevant MeSH Terms
Other Study ID Numbers
- 133959
Plan for Individual participant data (IPD)
Plan to Share Individual Participant Data (IPD)?
YES
IPD Plan Description
Any data generated from the proposed work that is presented in a peer reviewed journal will be uploaded, de-identified, into the Metabolomics Data Repository and Coordinating Center (DRCC) (U01) through UCSD; http://www.metabolomicsworkbench.org/nihmetabolomics/datasharing.html.
In addition, .raw
data that is presented in a peer reviewed journal will be archived indefinitely and made available upon request.
IPD Sharing Time Frame
Data will become available upon publication in peer-reviewed journals and will be made available indefinitely.
IPD Sharing Access Criteria
Active membership and username are required to access the raw metabolomics data on Metabolomics Workbench.
Drug and device information, study documents
Studies a U.S. FDA-regulated drug product
No
Studies a U.S. FDA-regulated device product
No
This information was retrieved directly from the website clinicaltrials.gov without any changes. If you have any requests to change, remove or update your study details, please contact register@clinicaltrials.gov. As soon as a change is implemented on clinicaltrials.gov, this will be updated automatically on our website as well.
Clinical Trials on Sleep Wake Disorders
-
Oregon State UniversityMonash University; Stanford University; American Academy of Sleep MedicineRecruitingSleep Initiation and Maintenance Disorders | Circadian Rhythm Sleep-Wake Disorders, Shift Work TypeUnited States
-
Vanda PharmaceuticalsCompletedNon-24-Hour-Sleep-Wake Disorder
-
Vanda PharmaceuticalsActive, not recruitingNon 24 Hour Sleep Wake DisorderFrance
-
Vanda PharmaceuticalsCompletedNon-24-Hour Sleep-Wake DisorderUnited States
-
Brigham and Women's HospitalActive, not recruitingSleep Disorders, Intrinsic | Sleep Disorders, Circadian Rhythm | Advanced Sleep Phase Syndrome (ASPS) | Delayed Sleep Phase Syndrome | Shift-Work Sleep Disorder | Delayed Sleep Phase | Non-24 Hour Sleep-Wake Disorder | Advanced Sleep Phase Syndrome | Advanced Sleep Phase | Irregular Sleep-Wake Syndrome | Shift...United States
-
University of Geneva, SwitzerlandCompletedNocturnal Leg Cramps | Sleep Wake Transition DisordersSwitzerland
-
Brigham and Women's HospitalCharite University, Berlin, Germany; Stanford UniversityRecruitingSleep Disorders, Intrinsic | Sleep Wake Disorders | Sleep Disorders, Circadian Rhythm | Advanced Sleep Phase Syndrome (ASPS) | Delayed Sleep Phase Syndrome | Shift-Work Sleep Disorder | Delayed Sleep Phase | Non-24 Hour Sleep-Wake Disorder | Advanced Sleep Phase Syndrome | Advanced Sleep Phase | Irregular... and other conditionsUnited States
-
Mayo ClinicCompletedDelayed Sleep-Wake Phase DisorderUnited States
-
Vanda PharmaceuticalsCompletedNon-24-Hour Sleep-Wake DisorderUnited States
-
Vanda PharmaceuticalsCompletedNon-24-Hour Sleep-Wake DisorderUnited States, Germany
Clinical Trials on Increased sleep duration
-
Texas Tech UniversityAmerican Heart AssociationCompleted
-
University of CopenhagenCompleted
-
Tokat Gaziosmanpasa UniversityRecruitingHealthy Young AdultTurkey
-
Brigham and Women's HospitalNational Institutes of Health (NIH)CompletedSleep | Circadian Rhythm | Menstrual CycleUnited States
-
University of ArizonaCompleted
-
The Miriam HospitalCompleted
-
King's College LondonGuy's and St Thomas' NHS Foundation TrustCompletedTic DisordersUnited Kingdom
-
University of OregonNational Institute on Alcohol Abuse and Alcoholism (NIAAA); University of PittsburghRecruitingAlcohol Use DisorderUnited States
-
University of OregonNational Institute of Mental Health (NIMH); University of Pittsburgh; Oregon...RecruitingDepression in AdolescenceUnited States
-
Temple UniversityCompletedSleep | Type2 DiabetesUnited States