Does a Multi-ingredient Pre-workout Supplement Enhance Cycling Time Trial Performance? (MIPS)

Does a Multi-ingredient Pre-workout Supplement Enhance Cycling Time Trial

The aim of the present study is to test the effect of ingesting a multi-ingredient pre- exercise supplement containing caffeine, creatine, beta-alanine and L-Citrulline compared to a placebo, on endurance exercise performance.

Study Overview

• Status: Completed
• Conditions: Healthy Volunteers
• Intervention / Treatment: Dietary supplement: Placebo; Dietary supplement: Ladder Supplement

• Study Type: Interventional
• Enrollment (Actual): 12
• Phase: Not Applicable

Contacts and Locations

Study Locations

• United Kingdom
  • Exeter, United Kingdom, EX4
    • School of Sport and Health Sciences

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 18 years to 40 years (Adult)
• Accepts Healthy Volunteers: Yes

Description

Exclusion criteria:

• Any diagnosed metabolic health condition
• Pregnant
• Non-habitual caffeine intake
• Smoker
• No endurance training
• Diagnosed Cardiovascular disease
• Recent history of musculoskeletal injury
• Anti-depressant and anti-anxiety medication

Inclusion criteria

• Age 18-40
• Healthy
• Highly trained endurance athletes (V̇O2max: males >55 and females >50 mL⋅kg bm-1⋅min-1)
• Both males and females are allowed to participate. If females are on the oral contraceptive pill they are to be tested on consecutive weeks. If they are not on the oral contraceptive pill, then female subjects are to be tested every 4 weeks, at the same point of their menstrual cycle.

Study Plan

How is the study designed?

Design Details

• Primary Purpose: Treatment
• Allocation: Randomized
• Interventional Model: Crossover Assignment
• Masking: Double

Number of Arms

2

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
Placebo Comparator: Placebo	Dietary supplement: Placebo; Placebo
Experimental: Ladder Supplement	Dietary supplement: Ladder Supplement; Caffeine 200mg L-Theanine 200mg L-Citrulline 6g Creatine 5g Beta-Alanine 1.6g Phosphatidylserine 100mg

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Exercise Performance	Work done during 15 min time trial (TT)	15 Minitues

Secondary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Alertness	Visual analog scale	1 Hour
Motivation	Visual analog scale	1 Hour
Rate of perceived exertion	Visual analog scale	1 Hour
Gas analysis	Analysis of expired gas	1 Hour

Collaborators and Investigators

• Sponsor: University of Exeter
• Collaborators: OpenFit LLC

Study record dates

Study Major Dates

• Study Start (Actual): December 2, 2021
• Primary Completion (Actual): August 31, 2022
• Study Completion (Actual): September 30, 2022

Study Registration Dates

• First Submitted: January 10, 2022
• First Submitted That Met QC Criteria: January 27, 2022
• First Posted (Actual): February 9, 2022

Study Record Updates

• Last Update Posted (Actual): July 31, 2025
• Last Update Submitted That Met QC Criteria: July 28, 2025
• Last Verified: July 1, 2025

More Information

Terms related to this study

• Other Study ID Numbers: 2021-M-38

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: No
• Studies a U.S. FDA-regulated device product: No