Evaluation of IL-17, TNF-alpha, RANKL Levels in Gingival Cervicular Fluid

Evaluation of IL-17, TNF-alpha, RANKL Levels in Gingival Cervicular Fluid of Teeth With Periapical Lesions Diagnosed With Chronic Apical Periodontitis

Inflammatory process begins around root apex as a result of bacterial infection of pulp cavity in chronic apical periodontitis. Bone destruction can begin in apical region after immunological reactions at the end of inflammatory process, and radiolucent periapical lesion seems in this way. If bone destruction around apical region is in the rate of 30%, we can notice this difference eventually. Histological manifestations of periapical inflammation can be observed in the result of pulp necrosis and inflammation around apical region. Mocelular methods can determine the type of therapy in various diseases. Each region and tissue reserve specific host factors. Vast majority of pulpal inflammation was trigerred by microorganisms. Certain bacterial virulence factors may damage host tissue directly, other virulence factors can stimulate prolonged non-specific immune response causing tissue damage. In the last phase of infection, immunopathological destruction of pulp tissue is observed due to humoral response. IL-17 is an important inflammatory cytokine released from T cells of the immune system. TNF-alpha and RANKL are also mediators responsible for bone destruction metabolism. TNF is a cytokine mediating immunologic changes during periodontal disease. TNF induction stimulate secondary mediators taking part as chemotactic cytokines. TNF has two different types; TNF-alpha and TNF-beta. TNF-alpha is a polypeptide cytokine produced by macrophages and monocytes. TNF-alpha stimulates bone resorption . There has been limited researches analyzing GCF, blood and tissue of pulp, dentin-derived fluid and periapical fluid for molecular diagnosis so far. GCF is a fluid derived from gingival groove. This fluid contains a lot of host factors such as anticor, bacterial antigen, protein and cytokines. GCF sampling is a non-invasive method, it can be used to provide diagnostic information in all clinical cases. In this study, we will evaluate the levels of IL-17, TNF-alpha and RANKL in the gingival crevicular fluid of teeth with periapical lesion diagnosed with chronic apical periodontitis. Thus, it will be evaluated whether these markers can be used for diagnosis and follow-up of the disease in teeth with periapical lesions.

Study Overview

• Status: Not yet recruiting
• Conditions: Apical Periodontitis
• Intervention / Treatment: Diagnostic test: The teeth diagnosed with chronic apical periodontitis

Detailed Description

It is planned to include volunteer patients who came to Van YYU Faculty of Dentistry, Oral and Maxillofacial Radiology Department between February and March 2022 for routine examination. Patients who applied to Yüzüncü Yıl University Faculty of Dentistry for treatment and met the study criteria will be included in the study by signing an informed consent form. 40 patients diagnosed with chronic apical periodontitis and having teeth with radiographic periapical lesions will be included in the study. Patients with periodontitis will not be included in the study.

GCF samples will be taken from the teeth diagnosed with chronic apical periodontitis and the contralateral teeth (from healthy teeth). The tooth which is resource of GCF will be isolated with cotton pellets. After the supragingival plaque is carefully removed with a curette, the tooth will be air-dried for 10 seconds, and periopaper strips will be placed in the gingival sulcus until resistance is felt. Periopaper strips will be removed from the gilgival sulcus after 1 minute. The levels of IL-17, TNF-alpha and RANKL from the samples in the gingival crevicular fluid will be evaluated.

• Study Type: Observational
• Enrollment (Anticipated): 40

Contacts and Locations

• Study Contact: Name: Hacer Aydınyurt, PhD; Phone Number: 905304589710; Email: hacersahin@gmail.com
• Study Contact Backup: Name: Murat Tunca, Phd; Phone Number: 905337141230; Email: dtmurattunca@gmail.com

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 18 years to 45 years (Adult)
• Accepts Healthy Volunteers: No
• Genders Eligible for Study: All

• Sampling Method: Probability Sample

Study Population

It is planned to include volunteer patients who came to Van YYU Faculty of Dentistry, Oral and Maxillofacial Radiology Department for routine examination.

Description

Inclusion Criteria:

• Healthy persons between the ages of 18 and 45 years
• Mandibular molar teeth that were diagnosed with symptomatic irreversible pulpitis and periapical lesion

Exclusion Criteria:

• Patients who are taken analgesic inflammatory drugs with in the last 12 hours
• Pregnancy or lactation
• Teeth with periodontal diseases
• Teeth with sensitive to percussion and palpation
• Teeth with root resorption
• Teeth with immature/open apex

Study Plan

How is the study designed?

Design Details

• Observational Models: Case-Only
• Time Perspectives: Cross-Sectional

Number of groups / cohorts

2

Cohorts and Interventions

Group / Cohort	Intervention / Treatment
1; GCF samples will be taken from the teeth diagnosed with chronic apical periodontitis	Diagnostic test: The teeth diagnosed with chronic apical periodontitis; GCF samples will be taken from the teeth diagnosed with chronic apical periodontitis
2; GCF samples will be taken from the collateral teeth (healthy teeth) of the teeth diagnosed with chronic apical periodontitis.	Diagnostic test: The teeth diagnosed with chronic apical periodontitis; GCF samples will be taken from the teeth diagnosed with chronic apical periodontitis

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
IL-17 Level	Concentration of IL-17 in GCF	6 months
TNF-alpha	Concentration of TNF-alpha in GCF	6 months
RANKL	Concentration of RANKL in GCF	6 months

Collaborators and Investigators

• Sponsor: Yuzuncu Yıl University

Publications and helpful links

General Publications

• Newton CW, Hoen MM, Goodis HE, Johnson BR, McClanahan SB. Identify and determine the metrics, hierarchy, and predictive value of all the parameters and/or methods used during endodontic diagnosis. J Endod. 2009 Dec;35(12):1635-44. doi: 10.1016/j.joen.2009.09.033.
• Ricucci D, Siqueira JF Jr, Bate AL, Pitt Ford TR. Histologic investigation of root canal-treated teeth with apical periodontitis: a retrospective study from twenty-four patients. J Endod. 2009 Apr;35(4):493-502. doi: 10.1016/j.joen.2008.12.014.

Study record dates

Study Major Dates

• Study Start (Anticipated): February 1, 2022
• Primary Completion (Anticipated): March 30, 2022
• Study Completion (Anticipated): April 30, 2022

Study Registration Dates

• First Submitted: January 17, 2022
• First Submitted That Met QC Criteria: February 1, 2022
• First Posted (Actual): February 10, 2022

Study Record Updates

• Last Update Posted (Actual): February 10, 2022
• Last Update Submitted That Met QC Criteria: February 1, 2022
• Last Verified: February 1, 2022

More Information

Terms related to this study

• Keywords: TNF-alpha; RANKL; Gingival cervicular fluid; IL-17
• Additional Relevant MeSH Terms: Stomatognathic Diseases; Periodontal Diseases; Mouth Diseases; Jaw Diseases; Periapical Diseases; Periodontitis; Periapical Periodontitis
• Other Study ID Numbers: 2021/08-02

Plan for Individual participant data (IPD)

• Plan to Share Individual Participant Data (IPD)?: No

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: No
• Studies a U.S. FDA-regulated device product: No
• product manufactured in and exported from the U.S.: No