Clinical Study of Tumor Polypeptide DC-CTL in the Treatment of Solid Tumors (DC-CTL)

This clinical study is a real-world exploratory study to observe the safety and initial effectiveness of DC-CTL cells combined with conventional anti-tumor therapy in patients with colorectal cancer, esophageal cancer, lung cancer, liver cancer, breast cancer and other solid tumors.

Study Overview

• Status: Not yet recruiting
• Conditions: Solid Tumor
• Intervention / Treatment: Biological: Tumor polypeptide pool induced DC-CTL cell injection

Detailed Description

This clinical study is a real-world exploratory study to observe the safety and initial effectiveness of DC-CTL cells combined with conventional anti-tumor therapy in patients with colorectal cancer, esophageal cancer, lung cancer, liver cancer, breast cancer and other solid tumors. In this study, DC-CTL cells were injected once a day for two consecutive days, 1-2 days after study participants received a conventional antitumor regimen, and subcutaneous injections of DC cells were administered before a second infusion of DC-CTL cells. A total of about 4-6 cycles of cell infusion are required, and the specific number of infusion and the composition of the peptide pool are determined by the investigator based on clinical needs and test results. After the cells have been transfused, their safety is examined first, and their effectiveness is evaluated during follow-up. According to the needs, this study plans to enroll about 20-200 patients with solid tumors such as colorectal cancer, esophageal cancer, lung cancer, liver cancer, and breast cancer. If the disease progresses during treatment, it is evaluated comprehensively by the investigator according to the clinical situation to determine whether the patient can be withdrawn from the study. Study participants in the follow-up phase who develop disease progression, begin other anti-tumor therapy without reaching disease progression after infusion, and voluntarily request to withdraw from the follow-up phase will be withdrawn from the follow-up phase and will be followed for survival only.

• Study Type: Interventional
• Enrollment (Estimated): 20
• Phase: Not Applicable

Contacts and Locations

• Study Contact: Name: Hui Zhao, PhD; Phone Number: 0371-63290608; Email: revogene@qq.com

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: Adult; Older Adult
• Accepts Healthy Volunteers: No

Description

Inclusion Criteria:

1. Age ≥18 years old, gender is not limited;
2. Patients clinically diagnosed with solid tumors such as colorectal cancer, esophageal cancer, lung cancer, liver cancer, breast cancer, etc., who need routine anti-tumor therapy;
3. KPS score ≥80 (Appendix 1), expected survival ≥3 months;
4. If imaging is available, the target lesion can be measured by conventional CT or MRI≥10 mm, and the short diameter of lymph node ≥15mm;
5. Liver function: TBIL≤3ULN, AST, ALT ≤2.5ULN; Renal function: Cr≤1.25ULN; Blood routine: WBC ≥ 4.0×109/L, Hb ≥90 g/L, PLT ≥ 80×109/L;
6. Good cardiac function (LVEF≥ 50%);
7. The peripheral superficial veins of the study participants were smooth, which could meet the needs of intravenous infusion;
8. No history of other malignant tumors, except for cured carcinoma in situ and papillary thyroid carcinoma;
9. Study participants agree to use a reliable contraceptive method for contraception from the time of signing the informed consent to the entire clinical study;
10. Study participants agree to participate in the clinical study and sign the Informed Consent form.

Exclusion Criteria:

1. Pregnant or lactating women (women of childbearing age need to do pregnancy tests);
2. Had a serious, uncontrolled infectious disease (bacterial, viral, or fungal infection) within 4 weeks prior to enrollment;
3. have chronic active hepatitis, AIDS or syphilis;
4. have a serious autoimmune disease or immunodeficiency disease (such as systemic lupus erythematosus, inflammatory bowel disease, multiple sclerosis, rheumatoid arthritis, psoriasis);
5. Severe allergy;
6. serious mental disorders;
7. suffering from serious heart, liver, kidney dysfunction, serious uncontrollable diabetes and other diseases;
8. Patients who had systematically used large amounts of glucocorticoids in the 4 weeks prior to enrollment (except those who used inhaled hormones);
9. Those who have previously been treated with other gene products;
10. Other investigators consider it inappropriate to participate in the study, or other circumstances that affect the analysis of the results of this clinical study.

Study Plan

How is the study designed?

Design Details

• Primary Purpose: Treatment
• Allocation: N/A
• Interventional Model: Single Group Assignment
• Masking: None (Open Label)

Number of Arms

1

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
Experimental: Treatment; Biological: DC-CTL DC combined with CTL	Biological: Tumor polypeptide pool induced DC-CTL cell injection; In this study, DC-CTL cells were injected once a day for two consecutive days, 1-2 days after study participants received a conventional antitumor regimen, and subcutaneous injections of DC cells were administered before a second infusion of DC-CTL cells. A total of about 4-6 cycles of cell infusion are required

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
PFS	progression free survival time	From the time of the first cell transfusion to the time of the subject's death due to any cause, up to 2 years after the subject received the last treatment

Secondary Outcome Measures

Outcome Measure	Measure Description	Time Frame
OS	over all survival time	From the time of the first cell transfusion to the time of the subject's death due to any cause, up to 2 years after the subject received the last treatment

Collaborators and Investigators

• Sponsor: Zhengzhou Revo-Gene Technology Co., LTD
• Collaborators: The First Affiliated Hospital of Zhengzhou University
• Investigators: Principal Investigator: Yi Zhang, professor, The first affiliated hospital of Zhengzhou university

Study record dates

Study Major Dates

• Study Start (Estimated): March 11, 2025
• Primary Completion (Estimated): April 1, 2026
• Study Completion (Estimated): April 1, 2026

Study Registration Dates

• First Submitted: July 16, 2024
• First Submitted That Met QC Criteria: July 24, 2024
• First Posted (Actual): July 29, 2024

Study Record Updates

• Last Update Posted (Actual): March 25, 2025
• Last Update Submitted That Met QC Criteria: February 5, 2025
• Last Verified: February 1, 2025

More Information

Terms related to this study

• Additional Relevant MeSH Terms: Neoplasms
• Other Study ID Numbers: RG2401

Plan for Individual participant data (IPD)

• Plan to Share Individual Participant Data (IPD)?: NO

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: No
• Studies a U.S. FDA-regulated device product: No