Aerobic Exercise on Platelet Mitochondrial Function

The Effects of Aerobic Exercise on Aerobic Fitness and Platelet Mitochondrial Bioenergetics

This study explores how exercise affects blood platelets and their role in clotting. Regular exercise can influence how efficiently clots form in the body. Intense exercise tends to increase platelet activity and the expression of certain molecules on their surface, while moderate exercise can decrease this activity. Since platelets are responsible for blood clotting, understanding how they function can be crucial.

Understanding Platelets:

Platelets are blood cells that help in clotting. The mitochondria within platelets help regulate their function. If these mitochondria don't work properly, it can lead to issues with clotting. By examining how exercise changes platelet mitochondria, the investigators can potentially identify important markers for health and disease progression.

Study Design:

This study compares two types of exercise routines: circuit training and stationary bicycle training.

Participants will be randomly assigned to one of the two groups:

Circuit Training Group: This involves moving through different exercise stations within a set time, targeting various body parts. It's tailored for those who might find regular exercise challenging, helping to reduce fatigue in any single area and improve overall fitness.

Bicycle Training Group: This involves exercising on a stationary bike. Both exercise groups will engage in high-intensity interval training for about 40 minutes, five times a week, over six weeks.

Assessment: Participants will undergo tests to measure endurance and physical function.

Blood Sampling: Blood will be drawn before and after the exercise tests, with 20 milliliters taken from the arm vein. Blood samples will also be collected at the start and end of a 6-week intervention, totaling 80 milliliters throughout the study. This helps the investigators analyze how platelets are affected by exercise.

This study is designed to help the investigators better understand how different types of exercise can benefit platelet function and overall health. Participanting in this research could contribute to the knowledge about how could exercise influence blood clotting and related health conditions. Participants' involvement will include exercise routines or maintaining your current lifestyle, along with simple blood tests. Participation is highly valuable and can help pave the way for new health insights and treatments.

Study Overview

• Status: Completed
• Conditions: Sedentary Lifestyle; Aerobic Exercise
• Intervention / Treatment: Other: EXERCISE TRAINING WITH OR WITHOUT MEDICATION

• Study Type: Interventional
• Enrollment (Actual): 36
• Phase: Not Applicable

Contacts and Locations

Study Locations

• Taiwan
  • Taoyuan, Taiwan, 333
    • Chang Gung University

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: Adult
• Accepts Healthy Volunteers: Yes

Description

Inclusion Criteria:

• Willing to sign a written informed consent form.
• Ages 18 to 28.
• No regular exercise habit (defined as exercising 2 times a week or less, for 60 minutes or less each time, for over a year).

Exclusion Criteria:

• Overweight (BMI > 24) or underweight (BMI < 19).
• Smoked within the past year, or consumed alcohol in the past month exceeding 60 grams of alcohol per occasion.
• Having metabolic, circulatory, respiratory, or immune-related diseases or risk factors such as diabetes, hyperlipidemia, hypertension, heart disease, clotting disorders, asthma, allergies, autoimmune diseases, etc.
• Long-term use of any nutritional supplements or medications (e.g., vitamins, pain relievers, aspirin, traditional Chinese medicine).
• Significant knee injuries within the past year (e.g., ligament damage, fractures).
• Students and laboratory staff taught, assessed, evaluated, tested, or appraised by the principal investigator of this study.

Study Plan

How is the study designed?

Design Details

• Primary Purpose: Basic Science
• Allocation: Randomized
• Interventional Model: Parallel Assignment
• Masking: None (Open Label)

Number of Arms

2

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
Experimental: Circuit training group	Other: EXERCISE TRAINING WITH OR WITHOUT MEDICATION; Participants in the exercise groups were trained five times a week for six weeks. The regimen began with a 5-min warm-up and ended with a 5-min cool-down, both at 40% of heart rate reserved (HRR). The circuit training participants were trained for three bouts of 10-min moderate-intensity exercise (60% of HRR) with 1-min rest intervals at a stationary ergometer, a rowing machine, and an elliptical trainer, respectively. The cycling training group was trained on an ergometer for three bouts of 10-min cycling on a stationary ergometer.
Active Comparator: Cycle ergometer group	Other: EXERCISE TRAINING WITH OR WITHOUT MEDICATION; Participants in the exercise groups were trained five times a week for six weeks. The regimen began with a 5-min warm-up and ended with a 5-min cool-down, both at 40% of heart rate reserved (HRR). The circuit training participants were trained for three bouts of 10-min moderate-intensity exercise (60% of HRR) with 1-min rest intervals at a stationary ergometer, a rowing machine, and an elliptical trainer, respectively. The cycling training group was trained on an ergometer for three bouts of 10-min cycling on a stationary ergometer.

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Platelet mitochondrial respiration	The mitochondrial OXPHOS and ET capacities in platelets were analyzed.	baseline and 2 days upon the completion of the 6-week intervention
Cardiopulmonary fitness	Graded Exercise Test was performed on an ergometer.	baseline and 2 days upon the completion of the 6-week intervention

Collaborators and Investigators

• Sponsor: Chang Gung Memorial Hospital

Study record dates

Study Major Dates

• Study Start (Actual): July 1, 2023
• Primary Completion (Actual): May 31, 2024
• Study Completion (Actual): May 31, 2024

Study Registration Dates

• First Submitted: March 19, 2025
• First Submitted That Met QC Criteria: March 20, 2025
• First Posted (Actual): March 27, 2025

Study Record Updates

• Last Update Posted (Actual): March 27, 2025
• Last Update Submitted That Met QC Criteria: March 20, 2025
• Last Verified: February 1, 2025

More Information

Terms related to this study

• Keywords: oxidative stress; physical exercise; thrombocytes; mitochondrial respiration
• Other Study ID Numbers: 202300538B0

Plan for Individual participant data (IPD)

• Plan to Share Individual Participant Data (IPD)?: UNDECIDED

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: No
• Studies a U.S. FDA-regulated device product: No
• product manufactured in and exported from the U.S.: No