Acute Response to Cigarette and E-cigarette Smoking Assesed in Exhaled Breath Condensate in Healthy Smokers of Traditional and E-cigarettes

The aim of the study is:

• to evaluate the acute response to traditional cigarettes on the respiratory system
• to evaluate the acute response to electronic cigarettes on the respiratory system
• to assess the relationship between the type of smoked cigarettes (traditional vs. electronic) and concentration of markers of airway inflammation released in healthy smokers

Material and methods:

• 29 healthy smokers of traditional cigarettes
• 29 healthy smokers of electronic cigarettes
• 29 healthy, nonsmoking volunteers

After 8 hours of smoking abstinence:

• the spirometry test
• the first collection of the exhaled breath condensate (EBC I)
• smoking a traditional / electronic cigarette
• the second collection of the exhaled breath condensate (EBC II) - one hour after smoking
• the third collection of the exhaled breath condensate (EBC III) - six hours after smoking
• the measurement of biomarkers of oxidative stress in the EBC (isoprostane-8, malondialdehyde MDA, IL-8) and pH of the EBC