Effects of Short-term Energy Deficit on Muscle Protein Turnover
2016年3月14日 更新者：Stuart M. Phillips、McMaster University
Weight loss through energy restriction results in the loss of both fat and muscle mass.
Muscle mass is important for mobility, strength, glucose control and energy expenditure, and therefore the retention of muscle mass during energy restriction is an important goal.
The retention of muscle mass depends on the balance between muscle protein synthesis (MPS) and muscle protein breakdown (MPB).
It is well known that MPS is reduced during weight loss, however the effect of weight loss on MPB is currently a topic of debate.
The purpose of this study is to assess the effect of short-term (10 day) weight loss (40% energy deficit) on both MPS and MPB in order to gain insight into the mechanisms causing muscle loss during energy restriction.
In addition, the effect of resistance exercise and protein (both known to preserve muscle mass) on MPS and MPB will be examined
調査の概要
詳細な説明
An undesirable consequence of energy restriction is the loss of muscle mass.
Muscle mass is determined by the the rates of two processes: muscle protein synthesis and muscle protein breakdown.
It has been consistently shown that a reduced rate of muscle protein synthesis exists during energy restriction.
However, it is currently unclear whether an increase in muscle protein breakdown also contributes to muscle mass loss.
In addition, the effect of high protein diets and resistance exercise on muscle protein turnover during energy restriction, two interventions known to preserve muscle, remains to be investigated.We aim to demonstrate that the decrease in muscle protein synthesis is the main contributor to a negative protein balance during energy restriction.
In addition, we hope to show that a higher-protein diet (2.4g/kg/d versus 1.2g/kg/d) and the addition of resistance exercise can help to maintain the muscle protein synthesis rate and thereby improve negative protein balance.The present study will be designed as a parallel group randomized controlled trial.
Subjects will be given pre-packaged diets (Copper County Foods) to consume which will put them in a relative energy deficit of 40% per day.
Based on random assignment, these subjects will either consume an adequate protein diet (1.2g/kg/d) or a high protein diet (2.4g/kg/d) for 10 consecutive days.
Participants in the adequate protein diet will consume a ratio of 50:35:15 (carbohydrates:fat:protein) while subjects within the high protein diet will consume a ratio of 50:15:35 (carbohydrates:fat:protein).
Subjects will undergo testing at baseline and following a 10 day dietary and unilateral resistance exercise intervention.
Testing will consist of muscle and blood samples.
Muscle protein synthesis and muscle protein breakdown will be measured using stable isotope tracers that are orally ingested (deuterated water) or infused into an antecubital vein (labelled phenylalanine tracers) before and after the 10 day diet.
研究の種類
介入
入学 (実際)
24
段階
- 適用できない
連絡先と場所
このセクションには、調査を実施する担当者の連絡先の詳細と、この調査が実施されている場所に関する情報が記載されています。
研究場所
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Ontario
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Hamilton、Ontario、カナダ、L8S 4K1
- Exercise Metabolism Research Laboratory, McMaster Univeristy
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参加基準
研究者は、適格基準と呼ばれる特定の説明に適合する人を探します。これらの基準のいくつかの例は、人の一般的な健康状態または以前の治療です。
適格基準
就学可能な年齢
20年～30年 (大人)
健康ボランティアの受け入れ
はい
受講資格のある性別
男
説明
Inclusion Criteria:
- Between the ages of 20 and 30 years inclusive
- Have a BMI between 25 and 33 inclusive
Exclusion Criteria:
- Have a body mass index less than 25 kg/m2 or greater than 33 kg/m2.
- Vegan
- History of allergy, sensitivity, or strong dislike towards dairy protein
- Smoker or user of tobacco products
- High physical activity
- Have health problems such as: renal or gastrointestinal disorders, metabolic disease, heart disease, vascular disease, rheumatoid arthritis, diabetes, poor lung function, uncontrolled blood pressure, dizziness, thyroid problems, or any other health conditions for which volunteers are being treated that might put them at risk for this study.
- Taking anti-diabetic, anti-inflammatory, platelet inhibitor, or anti-coagulant medications
- Use of an investigational drug product within the last 30 days
- Have participated in an infusion protocol in the last year
- Do not understand English or have a condition the PI believes would interfere with the ability to provide informed consent, comply with the study protocol, or which might confound the interpretation of the study results or put volunteers at undue risk
研究計画
このセクションでは、研究がどのように設計され、研究が何を測定しているかなど、研究計画の詳細を提供します。
研究はどのように設計されていますか？
デザインの詳細
- 主な目的：基礎科学
- 割り当て：ランダム化
- 介入モデル：並列代入
- マスキング：独身
武器と介入
参加者グループ / アーム |
介入・治療 |
|---|---|
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実験的：High Protein
2.4g protein/kg/d.
The macronutrient composition will be 50:15:35 (carbohydrates:fat:protein) during Energy Restriction
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All meals will be provided for the duration of the energy restriction intervention and the energy deficit will be calculated based on estimated energy requirements by indirect calorimetry to provide 60% of energy requirements.
In addition, one leg will be assigned to perform resistance exercise for 5 days during the 10 day energy deficit.
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実験的：Adequate Protein
1.2g protein/kg/d.
The macronutrient composition will be 50:35:15 (carbohydrates:fat:protein) during Energy Restriction
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All meals will be provided for the duration of the energy restriction intervention and the energy deficit will be calculated based on estimated energy requirements by indirect calorimetry to provide 60% of energy requirements.
In addition, one leg will be assigned to perform resistance exercise for 5 days during the 10 day energy deficit.
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この研究は何を測定していますか？
主要な結果の測定
結果測定 |
メジャーの説明 |
時間枠 |
|---|---|---|
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Muscle protein turnover
時間枠：Baseline and 10 days
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Change in muscle protein synthesis and muscle protein breakdown before and after the weight loss diet will be assessed.
The relationship between high protein diets (parallel groups) and exercise (unilateral resistance exercise) on muscle protein turnover will also be examined.
Muscle protein synthesis and muscle protein breakdown will be measured with stable isotope infusions of labelled phenylalanine tracers before and after the 10 day diet.
In addition, long term muscle protein synthesis will be measured with orally ingested deuterated water.
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Baseline and 10 days
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二次結果の測定
結果測定 |
メジャーの説明 |
時間枠 |
|---|---|---|
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Molecular markers
時間枠：Baseline and 10 days
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Change in the the transcriptional expression of genes involved in the ubiquitin-proteasome pathway (main regulator of muscle protein breakdown) measured in muscle samples obtained at baseline and following the 10 day dietary intervention by quantitative polymerase chain reaction (qPCR).
These same samples will be analyzed by western blot for the expression of active and inactive isoforms of caspase-3, an important component of muscle protein breakdown.
Finally, immunoprecipitation and western blot will be used to examine the ubiquitination of translation initiation factors at baseline and following the 10 day intervention
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Baseline and 10 days
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協力者と研究者
ここでは、この調査に関係する人々や組織を見つけることができます。
スポンサー
出版物と役立つリンク
研究に関する情報を入力する責任者は、自発的にこれらの出版物を提供します。これらは、研究に関連するあらゆるものに関するものである可能性があります。
一般刊行物
- Areta JL, Burke LM, Camera DM, West DW, Crawshay S, Moore DR, Stellingwerff T, Phillips SM, Hawley JA, Coffey VG. Reduced resting skeletal muscle protein synthesis is rescued by resistance exercise and protein ingestion following short-term energy deficit. Am J Physiol Endocrinol Metab. 2014 Apr 15;306(8):E989-97. doi: 10.1152/ajpendo.00590.2013. Epub 2014 Mar 4.
- Pasiakos SM, Cao JJ, Margolis LM, Sauter ER, Whigham LD, McClung JP, Rood JC, Carbone JW, Combs GF Jr, Young AJ. Effects of high-protein diets on fat-free mass and muscle protein synthesis following weight loss: a randomized controlled trial. FASEB J. 2013 Sep;27(9):3837-47. doi: 10.1096/fj.13-230227. Epub 2013 Jun 5.
- Pasiakos SM, Vislocky LM, Carbone JW, Altieri N, Konopelski K, Freake HC, Anderson JM, Ferrando AA, Wolfe RR, Rodriguez NR. Acute energy deprivation affects skeletal muscle protein synthesis and associated intracellular signaling proteins in physically active adults. J Nutr. 2010 Apr;140(4):745-51. doi: 10.3945/jn.109.118372. Epub 2010 Feb 17.
- Hector AJ, Marcotte GR, Churchward-Venne TA, Murphy CH, Breen L, von Allmen M, Baker SK, Phillips SM. Whey protein supplementation preserves postprandial myofibrillar protein synthesis during short-term energy restriction in overweight and obese adults. J Nutr. 2015 Feb;145(2):246-52. doi: 10.3945/jn.114.200832. Epub 2014 Dec 17. Erratum In: J Nutr. 2015 Jun;145(6):1373.
研究記録日
これらの日付は、ClinicalTrials.gov への研究記録と要約結果の提出の進捗状況を追跡します。研究記録と報告された結果は、国立医学図書館 (NLM) によって審査され、公開 Web サイトに掲載される前に、特定の品質管理基準を満たしていることが確認されます。
主要日程の研究
研究開始
2015年6月1日
一次修了 (実際)
2016年1月1日
研究の完了 (実際)
2016年1月1日
試験登録日
最初に提出
2015年3月28日
QC基準を満たした最初の提出物
2015年3月28日
最初の投稿 (見積もり)
2015年4月1日
学習記録の更新
投稿された最後の更新 (見積もり)
2016年3月16日
QC基準を満たした最後の更新が送信されました
2016年3月14日
最終確認日
2016年3月1日
詳しくは
この情報は、Web サイト clinicaltrials.gov から変更なしで直接取得したものです。研究の詳細を変更、削除、または更新するリクエストがある場合は、register@clinicaltrials.gov。 までご連絡ください。 clinicaltrials.gov に変更が加えられるとすぐに、ウェブサイトでも自動的に更新されます。
Energy Restrictionの臨床試験
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