パールエイジ研究。家族における腸内細菌の多世代伝播とその安定性。
腸内細菌の多世代伝播と家族内での安定性
Investigators will recruit up to 4-generations of human family cohorts, in order to characterize the microbiome and its changes across different generations.
調査の概要
状態
条件
詳細な説明
Interest in the gut microbiome from both the scientific community and public has increased exponentially over the last decade. Yet our knowledge of the microbes in the gut microbiome, their persistence and dispersal to new human hosts is still surprisingly ill-defined.
Investigators hypothesize that some gut microbes persist over multiple generations and are adapted to varying degrees to their human hosts and families, making them potentially important members of the human gut. Bacterial strains can persist in the human host for years, with maternally-transferred strains known to persist longer in infants than environmentally acquired ones. It is conceivable that a given strain can colonize a host for several vertical generations, as already shown for Bifidobacterium using cultured isolates. Yet investigators currently lack knowledge of microbial persistence over multiple generations, and the proposed PEARL-AGE cohort is the first study investigating multi-generation persistence of gut bacteria (to our best knowledge).
The PEARL-AGE project will investigate microbial transfer and the evolution of microbes in family members from different generations. Investigators will recruit siblings, fathers/guardians, grandparents, and great-grandparents to fully capture vertical (between generations) and horizontal (same generation) microbial transmission across multiple generations, as well as tracking parallel microbial evolution in multiple family members. This will substantially increase our understanding of microbial transmission, long-term microbial persistence through generations and between cohabiting family members and siblings.
研究の種類
入学 (推定)
連絡先と場所
研究場所
-
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Norfolk
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Norwich、Norfolk、イギリス、NR4 7UQ
- Quadram Institute Biosciences
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参加基準
適格基準
就学可能な年齢
- 子
- 大人
- 高齢者
健康ボランティアの受け入れ
受講資格のある性別
サンプリング方法
調査対象母集団
Investigators are recruiting the family members of PEARL (ClinicalTrials.gov ID: NCT03916874) study participants, a longitudinal study to understand how gut microbes contribute to maintaining health during pregnancy and early life already underway at QIB (Quadram Institute Bioscience). Investigators will extend this cohort, by recruiting family members of the PEARL mother to form the PEARL-AGE cohort, extending it from two generations to four generations.
説明
Inclusion Criteria:
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The following relations of the PEARL mother/baby are eligible for inclusion:
- Husband/partner of a PEARL mother, their biological parents and grandparents.
- PEARL mother's biological parents and grandparents (great-grandparents to the PEARL baby).
- Co-habiting non-biological fathers of the baby such as stepfathers and partners of the PEARL mother, their parents and grandparents.
- A biological sibling of the PEARL baby or a step-sibling who is currently co-habiting with the PEARL baby.
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Adult participants must be able to consent for themselves and able to understand the questionnaire.
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Child participants must have a parent and/or guardian to provide assent on their behalf.
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Must be living in England.
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Must be willing to accommodate a small cool box to store and transport the biological samples until they are collected, if not having immediate access to the PEARL study freezer.
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Vulnerable participants such as participants with mild cognitive impairment may be included as long as they have full support from their family members.
Exclusion Criteria:
- Not related/affiliated to a mother currently participating in the PEARL study, or the cohabiting partner, or father of the PEARL infant. If a PEARL participant has withdrawn from the PEARL study or is no longer participating, investigators will not approach the PEARL participant for recruitment of their family members.
- Step-fathers or long-term partners that have been cohabiting with the PEARL mother for less than three months.
- Those currently in prison.
- Those who are unable to provide written informed consent.
- Living with or related to a member of the Research Study team.
研究計画
研究はどのように設計されていますか？
デザインの詳細
- 観測モデル：COHORT
- 時間の展望：PROSPECTIVE
この研究は何を測定していますか？
主要な結果の測定
結果測定 |
メジャーの説明 |
時間枠 |
|---|---|---|
|
Characterizing microbial transfers in multigenerational families and subsequent stability of microbial colonization.
時間枠：2026
|
High-resolution metagenomics of strains tracked over time in individuals and families, relative to collected metadata (family closeness, medical history, etc) Associating microbiome stability to human genetic loci. |
2026
|
二次結果の測定
結果測定 |
メジャーの説明 |
時間枠 |
|---|---|---|
|
Characterizing gut microbial evolution and adaptation in human host families.
時間枠：2026
|
Comparative genomics of metagenome assembled genomes (MAGs) per individual. Identifying newly introduced mutations in microbial genomes. Observing parallel evolution (e.g., loss or gain of the same genes in bacteria). Associating human genotypes to microbial adaptation. |
2026
|
その他の成果指標
結果測定 |
メジャーの説明 |
時間枠 |
|---|---|---|
|
Describing the prokaryotic and eukaryotic genome-level diversity within the human gut
時間枠：2024
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Newly developed DNA extraction protocols for enrichment of specific microbial clades. |
2024
|
|
Identify predictors of within-family gut microbe transfer rates
時間枠：2026
|
Correlation coefficient of collected metadata (questionnaire derived) to observed shared microbes Correlate parallel colonization's of sibling to family questionnaire metadata (e.g. distances, closeness, habits, ..) Correlate genetic factors (immune system, etc) to microbial sharing/colonization rates |
2026
|
協力者と研究者
協力者
捜査官
- 主任研究者：Falk Hildebrand, PhD、Quadram Institute Bioscience
研究記録日
主要日程の研究
研究開始 (実際)
一次修了 (推定)
研究の完了 (推定)
試験登録日
最初に提出
QC基準を満たした最初の提出物
最初の投稿 (実際)
学習記録の更新
投稿された最後の更新 (実際)
QC基準を満たした最後の更新が送信されました
最終確認日
詳しくは
この情報は、Web サイト clinicaltrials.gov から変更なしで直接取得したものです。研究の詳細を変更、削除、または更新するリクエストがある場合は、register@clinicaltrials.gov。 までご連絡ください。 clinicaltrials.gov に変更が加えられるとすぐに、ウェブサイトでも自動的に更新されます。