Study Evaluating the Safety and Effects of MN-221 in Subjects With Moderate to Severe Asthma

A Randomized, Single-blind, Parallel Group, Placebo-controlled, Dose Rate Escalation Study Evaluating the Safety and Effects of MN-221 in Subjects Diagnosed With Moderate to Severe Asthma

The purpose of this study is to evaluate the safety and tolerability of MN-221 at two different dosing rates administered through a continuous infusion in subjects diagnosed with moderate to severe asthma.

Study Overview

• Status: Completed
• Conditions: Asthma
• Intervention / Treatment: Drug: MN-221; Drug: MN-221; Drug: Placebo

Detailed Description

This is a multi-center, randomized, single-blind*, parallel group, placebo-controlled, study with two dosing regimens in subjects diagnosed with moderate to severe asthma using MN-221 or placebo. Subjects will be randomized to receive MN-221 or placebo in a 3:1 ratio, MN-221:placebo. Subjects randomized to receive MN-221 will be dosed with active study drug at both dosing visits, and subjects randomized to the placebo arm will receive placebo at both dosing visits. Approximately 25 subjects diagnosed with moderate to severe asthma who have not received inhaled corticosteroid therapy within one month of Screen Visit 1 will be enrolled and will participate throughout the study.

Initial dose:

• 16 μg/min for 15 minutes followed by 8 μg/min for 105 minutes (2-hour infusion with a total dose of 1,080 μg MN-221 or Placebo)

Subsequent dose:

• 30 μg/min for 15 minutes followed by 15 μg/min for 45 minutes (1-hr infusion with a total dose of 1,125 μg MN-221 or Placebo)

There will be approximately a two to four week period between each dose regimen, during which time a safety review will be performed before proceeding to the next dose level. Subjects will be screened for eligibility and continued eligibility will be determined for each subject prior to administering each dose. After the initiation of the intravenous infusion of MN-221 or placebo, serial spirometry will be measured for approximately 24 hours.

For each dose evaluation period, subjects will be domiciled in the clinical research unit (CRU) for 2 nights, beginning on Day -1, one day before dosing. Determination of continued study eligibility will be made on Day -1 for each dose level. Day 1 will include study drug infusion and approximately a 24-hour observation period into Day 2 to allow safety monitoring, serial spirometry, and serum PK measurements. Subjects will be discharged from the CRU on Day 2. They will return to the CRU approximately 2-4 weeks later to participate in the subsequent dose group.

*This is a "modified" single-blind study in which the subject and Investigator are both blinded regarding the treatment arm.

• Study Type: Interventional
• Enrollment (Actual): 17
• Phase: Phase 2

Contacts and Locations

Study Locations

• United States
  • Kansas
    • Lenexa, Kansas, United States, 66219
      • PRA International
  • Massachusetts
    • North Dartmouth, Massachusetts, United States, 02747
      • Northeast Medical Research Associates
  • Missouri
    • Rolla, Missouri, United States, 65401
      • Clinical Research of the Ozarks
  • South Carolina
    • Greenville, South Carolina, United States, 29615
      • Greenville Pharmaceutical Research

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 18 years to 50 years (Adult)
• Accepts Healthy Volunteers: No
• Genders Eligible for Study: All

Description

Inclusion Criteria:

1. Male or female subjects;
2. Must sign an informed consent;
3. Diagnosis of asthma as defined by the American Thoracic Society for at least 3 months;
4. Must not be receiving inhaled corticosteroids for control within 1 month of study;
5. Must have an increase in FEV1 of at least 12% and 200 cc over the pre-albuterol FEV1 within 30 minutes after inhalation of up to 4 puffs of albuterol via a metered dose inhaler;
6. Non-smoker for at least 6 months and in good health;
7. Female subjects must have a negative pregnancy test;
8. Male and female subjects of child-bearing potential (not surgically sterile or post menopausal) must be abstinent or agree to use contraceptive regimens throughout the study;
9. Subjects receiving allergy desensitization therapy can participate as long as they have been on a stable maintenance dose for ≥ 6 months;
10. Subject must be willing and able not to use inhaled bronchodilators for 6 hours before and until 24 hours after each study drug administration at all study visits; and
11. Subject must demonstrate mental and physical ability and willingness to follow all study-specific instructions pertaining to the scheduling of study visits and assessments.

Exclusion Criteria:

1. Have significant cardiopulmonary, renal, hepatic, endocrine, metabolic, neurological or other systemic disease;
2. Received emergency treatment for asthma within 1 month, or hospitalized for asthma within 3 months;
3. Had an upper or lower respiratory tract infection within 3 weeks, or sinus infection within 7 days;
4. Have participated in a clinical study with an investigational drug, other than an MN-221 study, within 30 days;
5. Have history or evidence of drug or alcohol abuse within 2 years of study entry;
6. Female subjects who are pregnant or lactating;

7. Taking any of the following excluded asthma/allergy medications within the time periods indicated prior to the first study visit:

   • Oral, inhaled, or parenteral corticosteroids for 1 month prior to the first study visit.
   • Anti-IgE medication for 3 months prior to the first study visit.
   • Theophylline, long-acting bronchodilators, and anti-cholinergics for 2 weeks prior to the first study visit.
8. Taking leukotriene modifiers and not on a stable daily dosage for 4 weeks prior to the first study visit.
9. Taking antihistamines, nasal corticosteroids, or decongestants and not on a stable dose for 3 days prior to any dosing day.
10. Have a known allergy to MN-221 or any of the other components of the study drug (i.e. lactose);
11. Have a history of frequent episodes of orthostatic hypotension or any predisposition for orthostatic hypotension;
12. Have used any prescription or over-the-counter medication, vitamin or herbal supplement (except as listed in Inclusion Criterion 7 and Exclusion Criterion 7-10) within 7 days of study entry, or the expected need to use any unapproved prescription or over-the-counter medication or supplement seven days prior to the first study visit until completion of the study;
13. Taking any drugs or substances known to be strong inhibitors of cytochrome P450 enzymes within 10 days of study entry, or the expected need to use such drugs ten days prior to the first study visit until after completion of the study;
14. Taking any drugs or substances known to be strong inducers of cytochrome P450 enzymes within 28 days of study entry, or the expected need to use such drugs prior to completion of the study;
15. Adhering to any special diet that is not well balanced within 28 days of study entry;
16. Have donated (standard donation amount or more) blood or blood products (with the exception of plasma noted below) within 56 days of study entry;
17. Have donated plasma within 7 days of study entry;
18. Have any laboratory value outside the laboratory reference range that is considered to be clinically significant;
19. Have any abnormal vital signs (B/P, respiratory rate, heart rate) considered to be clinically significant;
20. Have positive results for drug and/or alcohol screen;
21. Have any clinically significant ECG abnormality, including a corrected QT interval (QTc) > 450 msec; and/or
22. Have any other medical condition or reason that, in the Investigator's opinion, makes the subject unsuitable to participate in this clinical trial.

Study Plan

How is the study designed?

Design Details

• Primary Purpose: Treatment
• Allocation: Randomized
• Interventional Model: Parallel Assignment
• Masking: Single

Number of Arms

2

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
Experimental: MN-221	Drug: MN-221; Initial dose: 16 μg/min for 15 minutes followed by 8 μg/min for 105 minutes (2-hour infusion with a total dose of 1,080 μg); Drug: MN-221; Subsequent dose: 30 μg/min for 15 minutes followed by 15 μg/min for 45 minutes (1-hr infusion with a total dose of 1,125 μg).
Placebo Comparator: PLACEBO; Placebo intravenous infusion with dosing volume equivalent to active treatment.	Drug: Placebo; Placebo intravenous infusion with dosing volume equivalent to active treatment.

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Time Frame
Number of Patients Reported to Have Adverse Events	Day 1 to Day 2

Secondary Outcome Measures

Outcome Measure	Measure Description	Time Frame
FEV1 Percent Predicted Changes From Base Line	The primary efficacy variable will be the change from baseline in FEV1, expressed as percent of predicted at hour 1 after the start of the infusion. Analysis of all other variables at all other time points will be considered secondary.	Day 1 to Day 2

Collaborators and Investigators

• Sponsor: MediciNova
• Investigators: Study Director: Michael Kalafer, MediciNova, Inc.

Publications and helpful links

General Publications

• Matsuda K, Makhay M, Johnson K, Iwaki Y. Evaluation of bedoradrine sulfate (MN-221), a novel, highly selective beta2-adrenergic receptor agonist for the treatment of asthma via intravenous infusion. J Asthma. 2012 Dec;49(10):1071-8. doi: 10.3109/02770903.2012.729631. Epub 2012 Oct 25.

Study record dates

Study Major Dates

• Study Start: February 1, 2008
• Primary Completion (Actual): July 1, 2008
• Study Completion (Actual): July 1, 2008

Study Registration Dates

• First Submitted: May 14, 2008
• First Submitted That Met QC Criteria: May 14, 2008
• First Posted (Estimate): May 16, 2008

Study Record Updates

• Last Update Posted (Estimate): December 19, 2011
• Last Update Submitted That Met QC Criteria: December 14, 2011
• Last Verified: November 1, 2011

More Information

Terms related to this study

• Keywords: safety; asthma; efficacy; randomized; placebo controlled; single blind; dose escalation; MN-221
• Additional Relevant MeSH Terms: Respiratory Tract Diseases; Immune System Diseases; Lung Diseases; Hypersensitivity, Immediate; Bronchial Diseases; Lung Diseases, Obstructive; Respiratory Hypersensitivity; Hypersensitivity; Asthma
• Other Study ID Numbers: MN-221-CL-005