Influence of Preconceptional Obesity on the Myometrial Cells Differentiation Toward a Contractyl Phenotype (ObeDi)

Study of Role of Mononuclear Cells of Maternal Peripheric Blood in Human Myometrial Primar Cell Lines Differentiation Toward a Contratyl Phenotype: Influence of Preconceptional Obesity

The purpose of this study is to improve the understanding of cellular mecanism involved in delivery disorders in obese women, first by characterizing the hyperplasia phenomenon of myometrail cells induced by inflammatory stimulation (Il-1B), then by studying the role of blood mononuclear cells, of leptine and of obese women sera to interfere with the transition of myometrial cells toward hyperplasia phenotype.

Study Overview

• Status: Completed
• Conditions: Pregnancy Complication
• Intervention / Treatment: Other: Myometrium biopsy and blood samples

• Study Type: Interventional
• Enrollment (Actual): 38
• Phase: Not Applicable

Contacts and Locations

Study Locations

• France
  • Dijon, France, 21000
    • Clinique Sainte Marthe
  • Bourgogne-Franche-Comté
    • Dijon, Bourgogne-Franche-Comté, France, 21000
      • Maternité du CHU de Dijon

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 18 years and older (Adult, Older Adult)
• Accepts Healthy Volunteers: Yes

Description

Inclusion Criteria:

• Informed consent signed
• Patient affiliated with a social security scheme
• In front of for obstetrical reasons to give birth by caesarean section (scheduled caesarean section)
• Or a woman who is not obese in labour and needs to undergo a cesarean section
• No obvious infectious context
• Age > 18 years old

Exclusion Criteria:

• Obvious inflammatory or autoimmune diseases
• Suspected or confirmed infectious context, including virale infection VIH, VHC, VHB
• Coagulation disorders

Study Plan

How is the study designed?

Design Details

• Primary Purpose: Basic Science
• Allocation: Non-Randomized
• Interventional Model: Parallel Assignment
• Masking: None (Open Label)

Number of Arms

3

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
Experimental: pre-conceptional obesity with a scheduled cesarean section	Other: Myometrium biopsy and blood samples; The biopsy of myometrium is specially realised for the study in surgical unit after the release of the child, in women with normal pregnancy but necessitating a caesarean. The blood samples are realised, specially for the study, either during the medical exam before the ceasarean or specifically for the study.
Active Comparator: non-obese women with a scheduled cesarean section	Other: Myometrium biopsy and blood samples; The biopsy of myometrium is specially realised for the study in surgical unit after the release of the child, in women with normal pregnancy but necessitating a caesarean. The blood samples are realised, specially for the study, either during the medical exam before the ceasarean or specifically for the study.
Active Comparator: women entering labour; women entering labour for vaginal delivery and for vaginal delivery	Other: Myometrium biopsy and blood samples; The biopsy of myometrium is specially realised for the study in surgical unit after the release of the child, in women with normal pregnancy but necessitating a caesarean. The blood samples are realised, specially for the study, either during the medical exam before the ceasarean or specifically for the study.

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Measuring the expression level of markers (Gama actine, Desmine, Connexine-43, and Ocytocine receptor) by Westernblotting	Demonstrate in vitro the capacity of a Il-1B stimulus to induce the differentiation of the myometrial cells toward a hyperplasia phenotype, and that the answer depends on whether this is cells from normal weight women or obese women before conception.	up to 2 months

Collaborators and Investigators

• Sponsor: Centre Hospitalier Universitaire Dijon

Publications and helpful links

General Publications

• Barrichon M, Hadi T, Wendremaire M, Ptasinski C, Seigneuric R, Marcion G, Delignette M, Marchet J, Dumas M, Sagot P, Bardou M, Garrido C, Lirussi F. Dose-dependent biphasic leptin-induced proliferation is caused by non-specific IL-6/NF-kappaB pathway activation in human myometrial cells. Br J Pharmacol. 2015 Jun;172(12):2974-90. doi: 10.1111/bph.13100. Epub 2015 Mar 24.

Study record dates

Study Major Dates

• Study Start (Actual): April 6, 2012
• Primary Completion (Actual): January 15, 2016
• Study Completion (Actual): January 15, 2016

Study Registration Dates

• First Submitted: May 31, 2012
• First Submitted That Met QC Criteria: January 7, 2013
• First Posted (Estimated): January 8, 2013

Study Record Updates

• Last Update Posted (Actual): February 9, 2026
• Last Update Submitted That Met QC Criteria: February 5, 2026
• Last Verified: February 1, 2026

More Information

Terms related to this study

• Keywords: obesity; pregnancy; differentiation
• Additional Relevant MeSH Terms: Urogenital Diseases; Nutrition Disorders; Female Urogenital Diseases and Pregnancy Complications; Overnutrition; Body Weight; Overweight; Pathological Conditions, Signs and Symptoms; Nutritional and Metabolic Diseases; Signs and Symptoms; Obesity; Pregnancy Complications; Investigative Techniques; Specimen Handling; Clinical Laboratory Techniques; Diagnostic Techniques and Procedures; Diagnosis; Punctures; Surgical Procedures, Operative; Blood Specimen Collection
• Other Study ID Numbers: LIRUSSI-AOI-2011