Circulatory Levels of Irisin in Response to Acute Resistance Training

Study the circulatory levels of irisin in response to one session of high-intensity resistance training in a group of healthy subjects.

Study Overview

• Status: Completed
• Conditions: Healthy
• Intervention / Treatment: Other: exercise training

Detailed Description

Objective: The purpose of this study is to study the circulating levels of irisin in response to acute high-intensity resistance training in a group of healthy young adults.

Sample: The sample will be comprised of adult men and women unpaid volunteers between the ages of 18 and 30. We will recruit 44 participants.

Pre- screening assessments: Informed consent, PAR-Q, Medical History and Exercise History will be used to determine health status and prior exercise experience. Menstrual cycle log will be required for female participants.

Pre-Intervention assessments (baseline):

• Anthropometric measurements and Body Composition: Height, triceps, thigh and calf skinfolds, biceps, thigh, hip, waist, and calf circumferences will be measured. Dual-energy x-ray absorptiometry (DEXA) will be performed to obtain body composition.
• Blood-related variables. Baseline glucose, lactate and hematocrit will be obtained from all participants prior to intervention. Lactate and Hematocrit will be obtained pre-intervention and post-intervention, and within 1 min post workout.

Irisin will be obtained from blood samples at baseline, during and after the training session. Irisin will be measured via a commercial enzyme-linked immunosorbent assay.

• Cardiopulmonary and Strength Fitness Testing. Cardiopulmonary fitness will be determined via maximum oxygen consumption testing. One maximum repetition (1-RM) will be used to assess the muscular fitness.
• Dietary Controls. Each participant will complete the Food Preference Questionnaire. In addition, the Automated Self-Administered 24-hour Recall (ASA24) will be collected for the day prior to the session.
• Familiarization. Participant will be instructed on the muscle conditioning techniques used for strength training so that the techniques can be mastered. The rate of perceived exertion (RPE) scale will also be used for familiarization during these sessions.
• Spontaneous Physical Activity: Daily activity level for 7 days will be collected.

Intervention: Resistance training will consist on a circuit training of 3 sets of 7 exercises focused on major muscle groups. Ten repetitions on each circuit at 70% of the maximum capacity will be performed. Acute intervention will consist of one session. Blood will be collected pre-session, at 45 min during the session, and immediately after the acute intervention exposure (post-session).

• Study Type: Interventional
• Enrollment (Actual): 28
• Phase: Not Applicable

Contacts and Locations

Study Locations

• United States
  • Texas
    • Lubbock, Texas, United States, 79423
      • Exercise and Sport Sciences Building

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 18 years to 30 years (Adult)
• Accepts Healthy Volunteers: Yes
• Genders Eligible for Study: All

Description

Inclusion Criteria:

• Participants between 18 and 30 years old
• Males and Females
• Healthy

Exclusion Criteria:

• Participants with known health problems such as cardiovascular, pulmonary or metabolic disease will be excluded.
• Pregnant females will be excluded from the study.
• Any adults found taking medications that affect endocrine or cardiovascular function will also be excluded from the study.
• Individuals found to be less than 110 pounds will also be excluded due to the collection of blood during this study.
• Participants that exercise more than three times per week or strength train more than twice per week will be excluded.
• Individuals that have poor venous access or have expressed a fear of needles or having blood taken will also be excluded.
• Cigarette smoking (current or those who quit within the previous 6 months
• Hypertension
• Overweight and obese
• Pre-diabetics

Study Plan

How is the study designed?

Design Details

• Primary Purpose: Basic Science
• Allocation: Randomized
• Interventional Model: Parallel Assignment
• Masking: None (Open Label)

Number of Arms

2

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
No Intervention: Control; No intervention
Experimental: Intervention; The acute intervention will consist of 1 session of training following the program: Resistance Training Exercises. Each participant in the intervention group will perform resistance-training exercises in the form of a circuit. The circuit will consist of 10 repetitions per exercise of 7 exercises: leg press, bent-over row, bench press, squats, dumbbell jump squats with raises, dead-lifts and weighted abdominal crunches, with approximately 30 sec of rest in between each exercise (based on the estimated time needed to move from one position to the next). Initial intensity 6-7 of RPE and ending the set at 9-10. Each participant will move through the circuit 3 times, with 2 to 3 minutes of rest between each round.	Other: exercise training; high-intensity resistance training

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Serum irisin levels	Changes in irisin levels baseline, 45 min (during the training session), and post-training session	1 day

Secondary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Body composition assessment, as measured by DEXA and anthropometry	Body composition at baseline prior to the training session	1 day
Lactate levels	Intensity of exercise at the end of the session (post-training session)	1 day
Physical Fitness levels as measured by 1 RM (repetition maximum) and Cardio-respiratory maximal test	Physical Fitness levels at baseline prior to the training session	1day
Blood Glucose levels	Blood Glucose levels at baseline prior to the training session	1 session (1 day)
Diet assessment: 24 hour recall and Food Preference Questionnaire	Dietary habits at baseline prior to the training session	1day
Hematocrit concentration	Hematocrit levels at baseline and post-training session	1 day
Physical Activity assessment, as measured by Accelerometry.	Quantification of baseline Physical Activity levels at baseline prior to the training session	1 week

Collaborators and Investigators

• Sponsor: Texas Tech University
• Investigators: Principal Investigator: Maria Fernandez del Valle, PhD, Texas Tech University

Study record dates

Study Major Dates

• Study Start: February 1, 2015
• Primary Completion (Actual): October 1, 2015
• Study Completion (Actual): December 1, 2015

Study Registration Dates

• First Submitted: February 3, 2015
• First Submitted That Met QC Criteria: June 18, 2015
• First Posted (Estimate): June 23, 2015

Study Record Updates

• Last Update Posted (Actual): July 25, 2019
• Last Update Submitted That Met QC Criteria: July 24, 2019
• Last Verified: July 1, 2019

More Information

Terms related to this study

• Keywords: Volunteers; resistance training; physical fitness; irisin
• Other Study ID Numbers: 504792A