Acute Exercise and Microvascular Function

Influence of Acute Exhausting Exercise on Microvascular Reactivity in Healthy Sedentary Subjects and Athletes

It is well recognized that chronic exercise protects against the development of cardiovascular diseases. However, the responses of vasculature to acute exercise (AE) are not well known and not consistent in literature. Acute exertional exercise can induce large, transient increases in arterial pressure and development of metabolic acidosis which can be associated with pro-inflammatory response involving oxidant stress and circulating cytokines known to impair endothelial function.

The aim of this study is to test the impact of a single exposure to exhausting training on microvascular reactivity in healthy sedentary subjects and athletes.

Study Overview

• Status: Unknown
• Conditions: Physical Exertion
• Intervention / Treatment: Other: Acute Exhausting Exercise (AE)

• Study Type: Interventional
• Enrollment (Anticipated): 30
• Phase: Not Applicable

Contacts and Locations

Study Locations

• Croatia
  • Osijek, Croatia, 31000
    • Recruiting
    • Faculty of Medicine Osijek, Laboratory for Clinical and Sport Physiology
    • Contact: Ana Stupin, MD, PhD; Phone Number: '385915134598; Email: anacavka@mefos.hr
    • Contact: Ines Drenjancevic, MD, PhD; Phone Number: '385912241406; Email: ines.drenjancevic@mefos.hr
    • Sub-Investigator: Marko Stupin, MD

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 18 years to 30 years (Adult)
• Accepts Healthy Volunteers: No
• Genders Eligible for Study: All

Description

Inclusion Criteria:

• healthy volunteers
• active athletes
• lean sedentary subjects

Exclusion Criteria:

• drugs that could affect the endothelium
• overweight
• hypertension
• coronary artery disease
• diabetes
• hyperlipidaemia
• renal impairment
• cerebrovascular and peripheral artery disease

Study Plan

How is the study designed?

Design Details

• Primary Purpose: Basic Science
• Allocation: Non-Randomized
• Interventional Model: Parallel Assignment
• Masking: None (Open Label)

Number of Arms

2

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
Experimental: Sedentary Subjects; Healthy lean sedentary subjects.	Other: Acute Exhausting Exercise (AE); A single progressive rowing training to maximal exhaustion (AE).
Experimental: Athletes; Active athletes.	Other: Acute Exhausting Exercise (AE); A single progressive rowing training to maximal exhaustion (AE).

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Skin microcirculation post occlusive reactive hyperemia	Cutaneous microvascular blood flow will be measured by Laser Doppler Flowmetry in response to vascular occlusion (post occlusive reactive hyperemia - PORH) before and after acute exhausting training.	Post occlusive reactive hyperemia - PORH, recorded by Laser Doppler Flowmetry will be measured immediately after acute exhausting training.
Skin microcirculation acetylcholine induced dilation	Cutaneous microvascular blood flow will be measured by Laser Doppler Flowmetry in response to iontophoresis of acetylcholine (acetylcholine induced dilation, AChID) before and after acute exhausting training.	Acetylcholine induced dilation (AChID), recorded by Laser Doppler Flowmetry will be measured immediately after acute exhausting training.

Collaborators and Investigators

• Sponsor: Josip Juraj Strossmayer University of Osijek
• Investigators: Principal Investigator: Ines Drenjancevic, MD, PhD, Faculty of Medicine, Josip Juraj Strossmayer University of Osijek

Publications and helpful links

General Publications

• Cavka A, Cosic A, Jukic I, Jelakovic B, Lombard JH, Phillips SA, Seric V, Mihaljevic I, Drenjancevic I. The role of cyclo-oxygenase-1 in high-salt diet-induced microvascular dysfunction in humans. J Physiol. 2015 Dec 15;593(24):5313-24. doi: 10.1113/JP271631. Epub 2015 Dec 7.
• Cavka A, Jukic I, Ali M, Goslawski M, Bian JT, Wang E, Drenjancevic I, Phillips SA. Short-term high salt intake reduces brachial artery and microvascular function in the absence of changes in blood pressure. J Hypertens. 2016 Apr;34(4):676-84. doi: 10.1097/HJH.0000000000000852.
• Phillips SA, Das E, Wang J, Pritchard K, Gutterman DD. Resistance and aerobic exercise protects against acute endothelial impairment induced by a single exposure to hypertension during exertion. J Appl Physiol (1985). 2011 Apr;110(4):1013-20. doi: 10.1152/japplphysiol.00438.2010. Epub 2011 Jan 20.

Study record dates

Study Major Dates

• Study Start: February 1, 2016
• Primary Completion (Anticipated): May 1, 2017
• Study Completion (Anticipated): December 1, 2017

Study Registration Dates

• First Submitted: March 18, 2016
• First Submitted That Met QC Criteria: March 29, 2016
• First Posted (Estimate): April 4, 2016

Study Record Updates

• Last Update Posted (Estimate): April 4, 2016
• Last Update Submitted That Met QC Criteria: March 29, 2016
• Last Verified: March 1, 2016

More Information

Terms related to this study

• Keywords: exercise; microvascular function; laser Doppler flowmetry
• Other Study ID Numbers: 215861071403

Plan for Individual participant data (IPD)

• Plan to Share Individual Participant Data (IPD)?: No

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: No
• Studies a U.S. FDA-regulated device product: No
• product manufactured in and exported from the U.S.: No