COLON-IM : Microbiota and Immune Infiltrate in Normal, Dysplastic and Neoplastic Colorectal Tissue

COLON-IM : Prospective Cohort Study About Colorectal Environment : Microbiota and Immune Infiltrate in Normal, Dysplastic and Neoplastic Colorectal Tissue

The primary objective of COLON-IM is to describe colorectal tissue microenvironment (neutrophils infiltrate) of patients with benign or malignant colorectal lesion (from stage I to III according to Tumor Node Metastasis (TNM)/ Union for International Cancer Control (UICC) classification).

Study Overview

• Status: Recruiting
• Conditions: Colorectal Cancer

Detailed Description

Colorectal cancers (CRC) are the most common gastrointestinal cancers in Western countries (both Europe and the US) ; they are both associated with significant morbidity and mortality. The 5-year survival is around 63.5% (including all stages). Sporadic colon cancers make up 85% of all colorectal adenocarcinoma. Preneoplastic lesions accumulate alterations in genes that regulate cell growth.

Patients suffering from CRC have a modified intestinal flora compared to healthy people. A specified microbiota profiled combined to genetic and immunological characteristics should be involved in colorectal carcinogenesis. Recent data have showed that responses to immunotherapies are associated with presence of certain bacteria in intestinal flora which potentially boost immune antitumor response.

Immunotherapy is highly efficient in tumors with high mutation load and Microsatellite Instability-High (MSI-H). MSI-H tumors represent 15% of local colorectal tumors. Today, it is important to understand all immune agents roles in colorectal carcinogenesis in order to describe new interesting immune checkpoints to target.

Tumor cells and tissues can escape immune surveillance and immune defense by several mechanisms. All immune cells subtypes present in the tumor, at the invasive tumor front and/or in tertiary lymphoid structures constitute the "immune context". The immune microenvironment has been shown to play a crucial role in disease progression, maintenance and resistance to therapy. All immune cells could play a pro-tumor or an anti-tumor role. For example, lymphocyte infiltrate is described as a prognostic factor in colorectal cancer : a tool (Immunoscore) has been validated as prognostic tool and it provides independent and superior prognostic value to TNM classification. This immune classification measures the host immune response at the tumor site and helps to guide treatment strategies.

Adaptative immunity is well known ; however, innate immunity should also play an early role during carcinogenesis. Role of neutrophils in the tumor microenvironment remains controversial, with evidence for both pro- and anti-tumor roles.Tumour-associated neutrophils (TANS) have an antitumorigenic in early stage of carcinogenesis or a protumorigenic functions in metastatic stage. Evidences indicate that neutrophils manifest functional plasticity during oncogenic process. Neutrophil-to-lymphocyte ratio is associated with patient prognosis ; a high ratio seems to be associated with poor prognostic.

Intestinal microbiota and immune infiltrate play key roles in colorectal microenvironment. Their interdependent interaction and their impacts on colorectal neoplasia and treatments are not well known. The primary objective of COLON-IM is to describe colorectal tissue microenvironment of patients with benign or malignant colorectal lesion (from stage I to III according to TNM/UICC classification).

• Study Type: Observational
• Enrollment (Estimated): 400

Contacts and Locations

• Study Contact: Name: Gwenaelle GARIN; Phone Number: 04 26 55 68 24; Email: gwenaelle.garin@lyon.unicancer.fr
• Study Contact Backup: Name: Matthieu SARABI, Dr; Phone Number: 04 69 85 75 82; Email: matthieu.sarabi@lyon.unicancer.fr

Study Locations

• France
  • Lyon, France, 69008
    • Recruiting
    • Centre Leon Berard
    • Contact: Lucas DE CRIGNIS, Dr; Phone Number: 04 78 78 28 28; Email: Lucas.decrignis@lyon.unicancer.fr@lyon.unicancer.fr
  • Lyon, France, 69004
    • Recruiting
    • Clinique de l'Infirmerie Protestante
    • Contact: GARBIT Vincent, Dr; Phone Number: 04 72 00 71 65; Email: vincent.garbit@infirmerie-protestante.com
  • Lyon
    • Lyon, Lyon, France, 69007
      • Recruiting
      • Hopital Saint Joseph Saint Luc
      • Contact: MILOU Fabrice, Dr; Email: fmilou@saintjosephsaintluc.fr

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 18 years and older (Adult, Older Adult)
• Accepts Healthy Volunteers: No

• Sampling Method: Non-Probability Sample

Study Population

Cohort A : with benign or malignant colorectal lesion (from stage I to III according toTNM/UICC classification) eligible to surgery, not previously be treated with an anticancer systemic agent (any type) and not be previously exposed to radiotherapy.

Cohort B : with localised colon/rectume adenocarcinoma, eligible to surgery, and treated by radiotherapy and/or pre-operative chemotherapy

Description

Inclusion Criteria:

• I1. Male or female patient 18 age or older at time of inform consent signature.
• I2. Patient Cohort A : with benign or malignant colorectal lesion (from stage I to III according to TNM/UICC classification) eligible to surgery, not previously be treated with an anticancer systemic agent (any type) and not be previously exposed to radiotherapy.

Cohort B : with localised colon/rectume adenocarcinoma, eligible to surgery, and treated by radiotherapy and/or pre-operative chemotherapy

• I3. Patient should be able and willing to comply with procedures as per protocol.
• I4. Patient should understand, sign, and date the written voluntary informed consent form prior to any protocol-specific procedures performed.
• I5. Patient must be covered by a medical insurance.

Exclusion Criteria:

• E1. Pregnant or breast-feeding female patient.
• E2. Prior treatment with : Any immunomodulatory treatment (streroids, immunosuppressive therapies) within 4 weeks prior inclusion, Any antibiotics within 8 weeks prior inclusion.
• E3. Patients with secondary malignancy unless this malignancy is not expected to interfere with the evaluation of study endpoints and is approved by the sponsor. Examples of the latter include: in-situ carcinoma of the cervix treated adequately, basal or squamous cell carcinoma of the skin. Patients previously treated for another cancer type and without evidence of relapse for at least 1 year are eligible.
• E4. Patient with inflammatory disease or autoimmune disease.
• E5. Patient under curatorship, guardianship or judicial protection.

Study Plan

How is the study designed?

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Characterization of colorectal tissue microenvironment of patients with locally colorectal neoplasia.	Rate of immune infiltrate in colorectal tissue	At surgery

Secondary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Transcriptome profiling by RNAseq of neutrophils associated with neoplastic and preneoplastic lesions.	mRNA level by RNAseq from neutrophils at different preneoplastic and neoplastic stages.	At surgery
Characterization of lymphocyte cells infiltrate in colorectal tissue (normal, neoplastic and preneoplastic).	Rate of lymphocyte cells in colorectal tissue (normal, neoplastic and preneoplastic).	At surgery
Evaluation of the correlation between immune infiltrate and clinical data, microsatellite status and clinical evolution.	Correlation between immune infiltrate (including neutrophils infiltrate) and clinical data (neoplastic lesion stage according to TNM/UICC classification and preneoplastic lesion (low or high dysplasia), microsatellite status, clinical outcome (survival without relapse and overall survival).	At surgery and through study completion, at least 42 months
Evaluation of cytokinic environment associated to neoplastic and preneoplastic lesions and evaluation of the correlation between cytokinic environment and clinical data, microsatellite status and clinical evolution.	Correlation between cytokinic environment and clinical data (neoplastic lesion stage according to TNM/UICC classification and preneoplastic lesion (low or high dysplasia), microsatellite status, clinical outcome (survival without relapse and overall survival)	At surgery and through study completion, at least 42 months
Identification of microbiota modifications in stools and mucosa during colic carcinogenesis	Sequences of the variable portions of the 16s ribosomal RNA gene	At surgery, at Month 6 post-surgery, at Month 9 post-surgery
Identification of potential molecular abnormalities in cancer cells from colorectal tissue and from blood samples.	Circulating tumor DNA and tumor DNA.	At surgery

Collaborators and Investigators

• Sponsor: Centre Leon Berard
• Investigators: Principal Investigator: Matthieu SARABI, Dr, Centre Leon Berard

Study record dates

Study Major Dates

• Study Start (Actual): May 2, 2019
• Primary Completion (Estimated): December 1, 2027
• Study Completion (Estimated): December 1, 2029

Study Registration Dates

• First Submitted: February 8, 2019
• First Submitted That Met QC Criteria: February 13, 2019
• First Posted (Actual): February 15, 2019

Study Record Updates

• Last Update Posted (Actual): April 24, 2026
• Last Update Submitted That Met QC Criteria: April 21, 2026
• Last Verified: April 1, 2026

More Information

Terms related to this study

• Keywords: Microbiota; Immune infiltrate; Microenvironment colorectal tissue
• Additional Relevant MeSH Terms: Neoplasms by Site; Neoplasms; Intestinal Diseases; Gastrointestinal Neoplasms; Digestive System Neoplasms; Digestive System Diseases; Gastrointestinal Diseases; Intestinal Neoplasms; Rectal Diseases; Colonic Diseases; Colorectal Neoplasms
• Other Study ID Numbers: ET18-276 (COLON-IM)

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: No
• Studies a U.S. FDA-regulated device product: No
• product manufactured in and exported from the U.S.: No