Antibacterial Activity of Urea Against Ocular Bacteria

In Vitro Evaluation of Antibacterial Activity of Urea Solution Against Ocular Bacterial Isolates

Antibacterial properties of urea was tested against 35 isolates from ocular infections.

Study Overview

• Status: Completed
• Conditions: Urea in Blood; High
• Intervention / Treatment: Biological: Antibacterial Activity

Detailed Description

In vitro susceptibility testing was performed with commercially available antibiotic discs, homemade urea discs saturated with urea solution at a concentration of 96 mmol. The weight of urea in one drop = 0.37 mg, in one ml = 10.34 mg (5 microliter/ disc) discs and combined discs (antibiotic discs to which urea solution was added). Combined discs provided better in vitro susceptibility results than antibiotics alone.

Scanning electron micrographs of bacterial isolates incubated overnight with urea solution showed marked disruption of bacterial aggregative patterns and irregularities of their cell walls.

Western blotting showed marked reduction in total proteins in bacterial isolates incubated overnight with urea solution. These findings may explain the possible mechanisms for antibacterial activity of urea solution as a result of protein denaturation.

• Study Type: Observational
• Enrollment (Actual): 90

Contacts and Locations

Study Locations

• Egypt
  • Assiut, Egypt
    • Hassan Lotfy Fahmy

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 5 months to 65 years (Child, Adult, Older Adult)
• Accepts Healthy Volunteers: No
• Genders Eligible for Study: All

• Sampling Method: Probability Sample

Study Population

individuals with any ocular infection

Description

Inclusion Criteria:

• ocular infection

Exclusion Criteria:

• Local antibiotics treatment

Study Plan

How is the study designed?

Design Details

• Observational Models: Cohort
• Time Perspectives: Prospective

Number of groups / cohorts

1

Cohorts and Interventions

Group / Cohort	Intervention / Treatment
Antibacterial Activity of Urea; Activity of Urea Solution against Ocular Bacterial Isolates	Biological: Antibacterial Activity; Antibacterial Activity of Urea Solution against Ocular Bacterial Isolates

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Antibacterial effect of Urea Solution against Ocular Bacterial	Samples were both inoculated on Muller Agar (Sigma-Aldrich) plates and directly examined under the microscope using Gram stained smears. Inoculated plates were incubated at 37oC for 48 hrs. Gram negative isolates were sub-cultured on MacConkey's, Eosin Methelyene Blue (EMB) and Cystine Lactose Electrolyte Deficient (CLED) agar plates (Sigma-Aldrich). Gram positive isolates were sub-cultured on Mannitol Salt agar plates (Sigma-Aldrich).	6 month

Collaborators and Investigators

• Sponsor: Assiut University
• Investigators: Principal Investigator: Hassan 58 Lotfy, Professor, Assiut T

Study record dates

Study Major Dates

• Study Start (Actual): November 1, 2017
• Primary Completion (Actual): November 1, 2018
• Study Completion (Actual): November 1, 2018

Study Registration Dates

• First Submitted: July 7, 2019
• First Submitted That Met QC Criteria: July 11, 2019
• First Posted (Actual): July 16, 2019

Study Record Updates

• Last Update Posted (Actual): July 16, 2019
• Last Update Submitted That Met QC Criteria: July 11, 2019
• Last Verified: July 1, 2019

More Information

Terms related to this study

• Additional Relevant MeSH Terms: Kidney Diseases; Urologic Diseases; Uremia; Anti-Infective Agents; Anti-Bacterial Agents
• Other Study ID Numbers: 2323297

Plan for Individual participant data (IPD)

• Plan to Share Individual Participant Data (IPD)?: Undecided

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: No
• Studies a U.S. FDA-regulated device product: No