Meteorin-like Levels in Individuals With Periodontitis

Evaluation of Meteorin-like Levels in Individuals With Healthy and Periodontal Disease

Metrnl has proven to be an inflammatory-related immunoregulatory cytokine and shown to play a role in the pathogenesis of human inflammatory diseases. Metrnl is highly expressed from the oral mucosa to the esophagus. Periodontitis is a chronic inflammatory disease that can start with localized inflammatory reactions created by the supporting tissues surrounding the teeth against microorganisms and then result in loss of teeth. The authors think that Metrnl may play a role in the periodontitis. The aim of this study is to compare the Metrnl, IL-1β, and IL-10 levels of healthy and periodontitis individuals.

Study Overview

• Status: Completed
• Conditions: Healthy; Periodontitis; Gingivitis
• Intervention / Treatment: Other: Saliva; Other: gingival crevicular fluid

Detailed Description

Periodontitis is a destructive chronic inflammatory disease that can start with localized inflammatory reactions created by the supporting tissues surrounding the teeth against microorganisms and then result in loss of teeth. Cytokines play an important role in inflammation that occurs in periodontal disease.

Interleukine -1β ( IL-1β ) levels are associated with periodontal destruction in the pathogenesis of periodontal disease. Cytokines such as Interleukine-10 (IL-10) and transforming growth factor- β (TGF-β) have been reported to decrease periodontal disease progression rate.

Gingival crevicular fluid shows the cellular response in the periodontium. The most important host-related ingredients are inflammatory markers, including cytokines, enzymes, and interleukins. Saliva analysis is used in the diagnosis of systemic conditions as well as in the diagnosis of oral pathologies.

Meteorin- like (Metrnl) is a protein that plays a role in natural and acquired immune-related functions and inflammatory responses, and expressed in barrier tissues (skin and mucosa) as well as various macrophage populations, from dendritic cells and some granulocytes. Metrnl has proven to be an inflammatory-related immunoregulatory cytokine and shown to play a role in the pathogenesis of human inflammatory diseases. Metrnl, which is highly expressed from the oral mucosa to the esophagus, may play a role in the onset of periodontitis and an effective marker in preventing the progression of destruction. We think that Metrnl plays an important role in periodontitis, an inflammatory disease. The aim of this study is to compare the Metrnl, IL-1β, and IL-10 levels of healthy and periodontitis individuals

• Study Type: Observational
• Enrollment (Actual): 60

Contacts and Locations

Study Locations

• Turkey
  • Eyalet/Yerleşke
    • İstanbul, Eyalet/Yerleşke, Turkey, 34668
      • Ebru Sağlam

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 18 years to 70 years (Adult, Older Adult)
• Accepts Healthy Volunteers: Yes

• Sampling Method: Probability Sample

Study Population

Individuals will be divided into three different groups, about 20-25 people in each group.

Description

Inclusion Criteria:

• systemically healthy
• individuals who does not have a disease under treatment,
• individuals without regular systemic medication

Exclusion Criteria:

• smoking
• receiving periodontal treatment in the last 6 months
• pregnancy
• menopausal or menstrual period

Study Plan

How is the study designed?

Number of groups / cohorts

3

Cohorts and Interventions

Group / Cohort	Intervention / Treatment
Periodontitis; Individuals with Periodontitis	Other: Saliva; individuals' saliva samples will be collected and transferred to eppendorf tubes. Until biochemical analysis is carried out will be stored in -80ºC.; Other Names: sampling; Other: gingival crevicular fluid; GCF samples of individuals will be collected by paper strips and transferred to eppendorf tubes. Until biochemical analysis is carried out will be stored in -80ºC.; Other Names: sampling
Gingivitis; Individuals with Gingival Inflammation	Other: Saliva; individuals' saliva samples will be collected and transferred to eppendorf tubes. Until biochemical analysis is carried out will be stored in -80ºC.; Other Names: sampling; Other: gingival crevicular fluid; GCF samples of individuals will be collected by paper strips and transferred to eppendorf tubes. Until biochemical analysis is carried out will be stored in -80ºC.; Other Names: sampling
Healthy; Individuals with Periodontally Healthy	Other: Saliva; individuals' saliva samples will be collected and transferred to eppendorf tubes. Until biochemical analysis is carried out will be stored in -80ºC.; Other Names: sampling; Other: gingival crevicular fluid; GCF samples of individuals will be collected by paper strips and transferred to eppendorf tubes. Until biochemical analysis is carried out will be stored in -80ºC.; Other Names: sampling

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
IL-1β	saliva and serum levels	through study completion, an average of 6 months
Metrnl	saliva and serum levels	through study completion, an average of 6 months
IL-10 levels	saliva and serum levels	through study completion, an average of 6 months

Secondary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Plaque Index	Silness-Löe	up to 20 weeks
Probing Depth	millimeter	up to 20 weeks
Bleeding on Probe	Ainamo&Bay	up to 20 weeks

Collaborators and Investigators

• Sponsor: Istanbul Saglik Bilimleri University
• Investigators: Study Chair: Ayşe Toraman, Dr., Saglik Bilimleri Universitesi; Principal Investigator: Ebru Sağlam, Dr, Saglik Bilimleri Universitesi; Study Chair: Serhat Köseoğlu, assoc prof, Saglik Bilimleri Universitesi; Study Chair: Mehmet Sağlam, assoc prof, İzmir Katip Çelebi University; Study Chair: Levent Savran, PhD, İzmir Katip Çelebi University

Study record dates

Study Major Dates

• Study Start (Actual): September 23, 2020
• Primary Completion (Actual): March 3, 2021
• Study Completion (Actual): March 3, 2021

Study Registration Dates

• First Submitted: May 19, 2020
• First Submitted That Met QC Criteria: May 21, 2020
• First Posted (Actual): May 27, 2020

Study Record Updates

• Last Update Posted (Actual): April 29, 2024
• Last Update Submitted That Met QC Criteria: April 25, 2024
• Last Verified: April 1, 2024

More Information

Terms related to this study

• Keywords: Meteorin-like
• Additional Relevant MeSH Terms: Infections; Stomatognathic Diseases; Periodontal Diseases; Mouth Diseases; Gingival Diseases; Periodontitis; Gingivitis
• Other Study ID Numbers: Sağlık Bilimleri Üniversitesi

Plan for Individual participant data (IPD)

• Plan to Share Individual Participant Data (IPD)?: NO

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: No
• Studies a U.S. FDA-regulated device product: No