Establishing Normative Values for Thermal Detection and Pain Threshold Established by the Psi Method

The study aims to compare different methods to assess thermal detection ability in diabetic patients, as a way to monitor and diagnose neurological complications of diabetes mellitus.

Study Overview

• Status: Suspended
• Conditions: Small Fiber Neuropathy
• Intervention / Treatment: Diagnostic test: Thermal QST (CDT, WDT, CPT, HPT) with the method of limits; Diagnostic test: Thermal QST (cold and warm detection, heat pain) with the psi method; Diagnostic test: Neurological examination

Detailed Description

Diabetic polyneuropathy is a frequent complication of diabetes mellitus. The impairment of peripheral nerve fibre function can be very variable, predominantly affecting large-diameter fibres (subserving touch), small-diameter fibres (subserving thermonociception), or both.

Thermal detection threshold evaluation can be used to quantify the extent of function loss (hypoesthesia) and, to a lesser extent, gain (hyperesthesia) in patients with thermonociceptive impairments. They are important features of quantitative sensory testing (QST) protocols (Rolke, Baron, et al., 2006; Rolke, Magerl, et al., 2006) and are pivotal to the determination of sensory phenotypes (Baron et al., 2017; Raputova et al., 2017). Their role is particularly important in the diagnostic workup of neuropathies affecting small fibers (i.e., the subgroup of primary afferents responsible for thermonociception and autonomic functions) such as painful diabetic neuropathies (Terkelsen et al., 2017; Tesfaye et al., 2010).

Currently, clinical measurements of thermal detection thresholds are mainly performed using the method of limits (Fruhstorfer, Lindblom, & Schmidt, 1976), in which a continuous heating or cooling ramp (usually at a slow rate, 1°C/s in the case of the DFNS QST protocol (Rolke, Magerl, et al., 2006)) is applied to the skin of the patient who is instructed to press a button as soon as he/she feels a warm or cold sensation. The detection threshold is then considered to be the temperature reached at the moment the patient pressed the button. The method of limits has been known for a long time to be methodologically biased due to its reliance on the reaction time (Yarnitsky & Ochoa, 1991), which lead to an overestimation of the threshold value corresponding to the temperature change that occurred between detection and it's signalling by a motor response. This is problematic as reaction times are under the influence of decision and motor reaction response speeds which may be affected by factors irrelevant to the assessment of sensory discrimination, such as cognitive or motor impairments.

A methodologically sounder approach for threshold measurement is the method of levels or constant stimuli (Kingdom & Prins, 2010). A number of preselected stimulus intensities are presented a number of times in random order and the subject is asked whether he/she felt each stimulus. Unlike the method of limits, this approach is not biased by decision speed and motor function. Furthermore, this method enables the fitting of a psychometric function (probability of detection as a function of stimulus intensity) to the results, therefore moving thermal detection performance assessments from the outdated High Threshold Theory framework to that of the currently leading Signal Detection Theory (Kingdom & Prins, 2010). Whereas High Threshold Theory conceptualized detection as an ON/OFF process (below threshold, no detection occurs, above threshold detection always occurs), Signal Detection Theory sees detection as a probabilistic process (each stimulus intensity is associated with a probability of detection). This theoretical framework implies to redefine the threshold as the stimulus intensity for which detection probability equates 0.5. In addition to the threshold, the psychometric function is also defined by its slope, i.e. the rate at which detection probability changes around the value of the threshold. . Unfortunately, the method of levels has some important drawbacks. First, it is time consuming as it requires collecting responses to a large amount of stimuli (usually several hundreds) (Gescheider, 1997). Second, the range of stimulus intensities must be approximately centered on the actual threshold value and cover the transition range of detection probability.

To overcome these limitations, several adaptive procedures have been proposed. These procedures actively adjust the intensity of the presented stimuli depending on the previous responses of the subject (Kingdom & Prins, 2010). In the present study, we implemented for the first time the Psi method (a Bayesian adaptive algorithm proposed by Kontsevich and Tyler (1999)) to estimate the thresholds and slopes of the psychometric function for heat and cold detection. This algorithm associates each potential values of slope and threshold with a probability, updates this probability distribution based on the response recorded after each stimulus presentation (detected/not detected), and selects the next stimulus intensity so that the response to its presentation maximizes the entropy (i.e. the uncertainty around the values of slope and threshold) reduction.

In this study, we will test healthy controls with the conventional method of limit and the new psi method, in order to establish normative values for the new test.

• Study Type: Interventional
• Enrollment (Anticipated): 80
• Phase: Not Applicable

Contacts and Locations

Study Locations

• Belgium
  • Brussels, Belgium, 1200
    • Institute of Neuroscience

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 36 years to 75 years (Adult, Older Adult)
• Accepts Healthy Volunteers: No
• Genders Eligible for Study: All

Description

Inclusion Criteria:

-

Exclusion Criteria:

• Alcohol beverage intake >3 units/day
• Habitual substance abuse
• History of chemotherapy
• Scar or dermatological condition at the site of stimulation (forearm and hands, leg and foot)
• History of neurological, psychiatric or metabolic disorder other than Diabetes Mellitus (screening will be performed with the patient)
• Currently taking drugs that could induce neuropathy (screening will be performed with the patient)
• For healthy controls: Suffering of chronic pain

Study Plan

How is the study designed?

Design Details

• Primary Purpose: Diagnostic
• Allocation: N/A
• Interventional Model: Single Group Assignment
• Masking: None (Open Label)

Number of Arms

1

Arms and Interventions

Participant Group / Arm

Intervention / Treatment

Experimental: Main study; Several electrophysiological and behavioural tests will be performed to properly diagnose the patients/check that the healthy controls do not suffer of neuropathy.

Diagnostic test: Thermal QST (CDT, WDT, CPT, HPT) with the method of limits; The testing will be carried out using the classical method of limits, i.e. a continuous heating/cooling stimulus will be applied to the skin at a rate of 1°C/s, until the subject signals that he/she felt the targeted sensation by pressing a button (Rolke, Baron, et al., 2006). The temperature reached by the time the subject pressed the button will be considered the threshold. Baseline temperature will be set at 32°C. The stimuli will not go lower than 0°C and higher than 50°C.; Diagnostic test: Thermal QST (cold and warm detection, heat pain) with the psi method; The Psi algorithm is a method using Bayesian statistics to determine not only the threshold but also the slope of the psychometric function (relationship between the intensity of a stimulus and its detection probability; Kingdom & Prins, 2010). The algorithm selects the next stimulus intensity to be the most informative on the parameters of interest, based on the prior probability density. After each stimulation, the subject will be asked if he/she felt it (as painful) or not. Based on that answer, a posterior probability density is computed. This posterior is then used as prior for the next stimulation. The stimuli will last maximum 1 s and temperature will be kept between 0°C and 60°C.; Diagnostic test: Neurological examination; A standardized neurological examination will be performed to assess all the items included in the Utah Early Neuropathy Scale (UENS ) (Singleton et al., 2008) and the Toronto Clinical Neuropathy Score (TCNS)(Perkins, Olaleye, Zinman, & Bril, 2001). This will include: questions about symptoms (presence or absence of foot pain, numbness, tingling, weakness, imbalance and upper limb symptoms); evaluation of knees and ankles deep tendon reflexes; evaluation of pinprick, temperature, light touch, vibration (128 Hz tuning fork) compared to that of an unaffected sites (e.g., sternum); position sensation in the big toes; mapping of pinprick sensitivity in the lower leg; and evaluation of the active extension of the big toes. Pinprick evaluation will be performed using a disposable pin designed for that purpose (Medipin, The United-Kingdom).

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Fitted psychometric functions for cold detection obtained with the psi method	The alpha (threshold; °C) and beta (slope, °C^-1) terms of the fitted logistic equation	baseline
Fitted psychometric functions for warm detection obtained with the psi method	The alpha (threshold; °C) and beta (slope, °C^-1) terms of the fitted logistic equation	baseline
Fitted psychometric functions for cold pain obtained with the psi method	The alpha (threshold; °C) and beta (slope, °C^-1) terms of the fitted logistic equation	baseline
Fitted psychometric functions for heat pain obtained with the psi method	The alpha (threshold; °C) and beta (slope, °C^-1) terms of the fitted logistic equation	baseline
Cold detection threshold (protocol of the German Research Network on Neuropathic Pain - DFNS)	average of 3 measurement of the threshold with the method of limits, as described in the protocol of the German Research Network on Neuropathic Pain - DFNS	baseline
Cold pain threshold (protocol of the German Research Network on Neuropathic Pain - DFNS)	average of 3 measurement of the threshold with the method of limits, as described in the protocol of the German Research Network on Neuropathic Pain - DFNS	baseline
Warm detection threshold (protocol of the German Research Network on Neuropathic Pain - DFNS)	average of 3 measurement of the threshold with the method of limits, as described in the protocol of the German Research Network on Neuropathic Pain - DFNS	baseline
Heat Pain threshold (protocol of the German Research Network on Neuropathic Pain - DFNS)	average of 3 measurement of the threshold with the method of limits, as described in the protocol of the German Research Network on Neuropathic Pain - DFNS	baseline

Other Outcome Measures

Outcome Measure	Measure Description	Time Frame
Utah Early Neuropathy Scale	The UENS is a score derived from standard neurological examination items (evaluation of ankles deep tendon reflexes; evaluation of vibration sensation (128 Hz tuning fork); evaluation of allodynia; evaluation of position sensation in the big toes; mapping of pinprick sensitivity in the lower leg; and evaluation of the active extension of the big toes), ranging from 0 to 42.	baseline
Toronto clinical neuropathy score	The UENS is a score derived from standard neurological examination items (questions about symptoms (presence or absence of foot pain, numbness, tingling, weakness, imbalance and upper limb symptoms); evaluation of knees and ankles deep tendon reflexes; evaluation of pinprick, temperature, light touch, vibration (128 Hz tuning fork) compared to that of an unaffected sites (e.g., sternum); position sensation in the big toes), ranging from 0 to 19.	baseline

Collaborators and Investigators

• Sponsor: Université Catholique de Louvain
• Collaborators: Cliniques universitaires Saint-Luc- Université Catholique de Louvain
• Investigators: Principal Investigator: André Mouraux, MD, PhD, Université Catholique de Louvain

Study record dates

Study Major Dates

• Study Start (Actual): October 10, 2020
• Primary Completion (Anticipated): July 1, 2022
• Study Completion (Anticipated): January 1, 2023

Study Registration Dates

• First Submitted: July 23, 2020
• First Submitted That Met QC Criteria: October 26, 2020
• First Posted (Actual): November 2, 2020

Study Record Updates

• Last Update Posted (Actual): October 8, 2021
• Last Update Submitted That Met QC Criteria: September 30, 2021
• Last Verified: September 1, 2021

More Information

Terms related to this study

• Additional Relevant MeSH Terms: Nervous System Diseases; Neuromuscular Diseases; Peripheral Nervous System Diseases; Small Fiber Neuropathy
• Other Study ID Numbers: PsiNorm

Plan for Individual participant data (IPD)

• Plan to Share Individual Participant Data (IPD)?: Yes
• IPD Plan Description: SAP will be available on the OSF repository by January 2021. Anonymized data will be freely available (stored on an OSF repository) as supporting material for publication.
• IPD Sharing Time Frame: SAP will be available on the OSF repository by January 2021. Anonymized data will be freely available (stored on an OSF repository) as supporting material for publication.
• IPD Sharing Access Criteria: No restriction
• IPD Sharing Supporting Information Type: Study Protocol; Statistical Analysis Plan (SAP)

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: No
• Studies a U.S. FDA-regulated device product: No