The Effect of Resistance Exercise on Inflammation in Young and Older Adults

The Acute Effect of High-load Resistance Exercise on Inflammation in Young and Older Adults

Healthy young and older adults will complete two trials in a randomized crossover counter-balanced order, including resistance exercise and sedentary control. During each trial, blood samples will be collected.

The investigators hypothesized that an acute bout of resistance exercise exert different inflammation responses in young and older adults.

Study Overview

• Status: Completed
• Conditions: Inflammatory Response; Aging
• Intervention / Treatment: Other: Resistance exercise

• Study Type: Interventional
• Enrollment (Actual): 16
• Phase: Not Applicable

Contacts and Locations

Study Locations

• Taiwan
  • Taipei, Taiwan, 162
    • National Taiwan Normal University

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 20 years to 80 years (Adult, Older Adult)
• Accepts Healthy Volunteers: No
• Genders Eligible for Study: Male

Description

Inclusion Criteria:

• Age: 20-80
• Healthy male
• Without experience of resistance training

Exclusion Criteria:

• No acute or chronic musculoskeletal symptoms
• Smoking
• Alcohol or drug abuse
• Fail to conduct resistance exercise

Study Plan

How is the study designed?

Design Details

• Primary Purpose: Treatment
• Allocation: Randomized
• Interventional Model: Crossover Assignment
• Masking: None (Open Label)

Number of Arms

2

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
No Intervention: Sedentary control; Subjects in control trial stayed sedentary during the period.
Experimental: High-load resistance exercise; Subjects in high-load trial performed 3 sets per exercise, 8 repetitions with load of 70%-1RM with 90 sec of rest between sets, followed by a fourth set to voluntary failure.	Other: Resistance exercise; 4 total resistance exercises that target all major muscle groups.

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Changes in biomarkers of inflammation	Biomarkers of inflammation will be measured such as IL-10 in pg/mL, IL-6 in pg/mL, TNF-α in pg/mL.	24-hour during each study intervention

Secondary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Borgs Ratings of Perceived Exertion (RPE)	Borgs RPE is a method of estimating exertion rating, based on a 6 to 20 rating scale (6 being the no exertion at all, 20 being maximal exertion).	1-hour during each exercise intervention
Changes in glucose	glucose in mg/dL	24-hour during each study intervention
Changes in metabolic parameters	Metabolic parameters will be measured such as TC in mg/dL, TG in mg/dL, and HDL in mg/dL.	24-hour during each study intervention
Changes in biomarkers of muscle damage	Biomarkers of muscle damage will be measured such as CK in U/L and LDH in U/L.	24-hour during each study intervention
Changes in molecular markers in peripheral blood mononuclear cells (PBMC)	Molecular markers in PBMC will be measured such as STAT3 in arbitrary units, IKK in arbitrary units, IkB in arbitrary units, p65 in arbitrary units, SOCS3 in arbitrary units, AMPK in arbitrary units, and SIRT1 in arbitrary units.	24-hour during each study intervention
Changes in complete blood count (CBC)	The white blood cell count and the count of each white blood cell type will be measured.	24-hour during each study intervention

Collaborators and Investigators

• Sponsor: National Taiwan Normal University
• Investigators: Principal Investigator: Hung-wen Liu, Ph.D., National Taiwan Normal University

Study record dates

Study Major Dates

• Study Start (Actual): October 1, 2021
• Primary Completion (Actual): December 31, 2022
• Study Completion (Actual): December 31, 2022

Study Registration Dates

• First Submitted: September 2, 2021
• First Submitted That Met QC Criteria: September 10, 2021
• First Posted (Actual): September 13, 2021

Study Record Updates

• Last Update Posted (Estimate): January 13, 2023
• Last Update Submitted That Met QC Criteria: January 12, 2023
• Last Verified: January 1, 2023

More Information

Terms related to this study

• Additional Relevant MeSH Terms: Pathologic Processes; Inflammation
• Other Study ID Numbers: 202012HM020

Plan for Individual participant data (IPD)

• Plan to Share Individual Participant Data (IPD)?: No

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: No
• Studies a U.S. FDA-regulated device product: No