Acute Impact of Different Foods and Beverages on Exercise Induced Oxidative Stress and Inflammation

The aim of this research project is to explore the acute impact of specific foods and beverages, which have been shown to be associated with inflammatory processes, mainly in epidemiological studies, on inflammatory and oxidative stress parameters in healthy humans following a high intensity physical workout.

Study Overview

• Status: Enrolling by invitation
• Conditions: Inflammatory Response; Oxidative Stress; Oxidative Stress Induction
• Intervention / Treatment: Other: Nutrition

Detailed Description

The aim of this research project is to explore the acute impact of specific foods and beverages, which have been shown to be associated with inflammatory processes, mainly in epidemiological studies, on inflammatory and oxidative stress parameters in healthy humans following a high intensity physical workout. The choice of food will mainly be based on the food groups from the Empirical Dietary Inflammatory Index. For that purpose, seventy-two healthy volunteers will be recruited to perform several interventions in order to assess the impact of different nutrient groups or single- foods on oxidative and inflammatory markers. They will be divided into the three groups beverage consumption, plantbased foods and protein sources. The intense physical activity serves to induce a physiological stress response in the body, known to be accompanied by an increase in oxidative and inflammatory stress parameters. The idea is that anti- inflammatory or antioxidant nutrients could potentially attenuate this stress response in dependence on their anti- inflammatory or antioxidant potential. The operability of this concept has already been tested. The main benefit of this approach is to get a deeper insight into short term effects of single foods or beverages on inflammatory and prooxidative processes in humans by using short term intense physical activity as a stress model. One major practical aspect of this approach is to assess dietary components with respect to their pro- or anti-inflammatory-/oxidative stress potential regarding e.g. non-communicable diseases such as type 2 diabetes, coronary heart disease or the metabolic syndrome. Furthermore, it could help to reduce inflammation or oxidative stress during or following high intense physical exercise in order to reduce the stress response during training or competition in order to enhance regeneration and to foster the training gain in sports.

• Study Type: Interventional
• Enrollment (Estimated): 24
• Phase: Not Applicable

Contacts and Locations

• Study Contact: Name: Markus Gassner, MSc; Phone Number: +436502007014; Email: markus.gassner@univie.ac.at
• Study Contact Backup: Name: Daniel König, Univ Prof; Phone Number: +43-1-4277-59130; Email: daniel.koenig@univie.ac.at

Study Locations

• Austria
  • Vienna, Austria, 1070
    • University of Vienna

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 18 years to 40 years (Adult)
• Accepts Healthy Volunteers: Yes

Description

Inclusion Criteria:

• female human subjects
• aged 18-40 years
• no chronical diseases
• not more than 120 minutes of physical activity per week
• no regular resistance training within the past 6 months
• BMI 18.5 - 24.99 kg/m²
• body fat percentage of at least 20%
• Exclusion Criteria:
• contraindications against physical activity
• anemia
• fructose or lactose intolerance
• smoking
• medications with influence on ROS levels or inflammation
• allergy or intolerance against intervention food/beverages.

Study Plan

How is the study designed?

Design Details

• Primary Purpose: Prevention
• Allocation: Randomized
• Interventional Model: Crossover Assignment
• Masking: None (Open Label)

Number of Arms

3

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
Experimental: Impact of GT, coffee, OJ, PGJ, milk, SSB on exercise induced oxidative stress&inflammation; single consumption of one cup of green tea (GT), coffee, orange juice (OJ), pomegranate juice (PGJ), milk and sugar sweetened beverage (SSB) 2 hours prior to a 30-min- HIIT resistance circuit exercise in healthy human subjects	Other: Nutrition; acute single dose nutrition intervention
Experimental: Impact of r&p meat, cheese, eggs, tofu, salmon, on exercise induced oxidative stress&inflammation; single consumption of one proportion of red meat (r meat), full fat cheese, eggs, tofu, salmon and processed meat (p meat) 2 hours prior to a 30-min- HIIT resistance circuit exercise in healthy human subjects	Other: Nutrition; acute single dose nutrition intervention
Experimental: Impact of choc, bcc, fl-oil, blueb., r&w bread on exercise induced oxidative stress&inflammation; single consumption of one proportion of dark chocolate (choc), broccoli (bcc), flaxseed oil, blueberries, white bread and whole grain bread (r = refined & w = whole grain) 2 hours prior to a 30-min-HIIT resistance circuit exercise in healthy human subjects	Other: Nutrition; acute single dose nutrition intervention

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
ROS change	ROS (change from baseline = after overnight fasting to post exercise (=after 2.5 hours)	change from baseline to 2.5 hours
MDA change	MDA (change from baseline = after overnight fasting to post exercise (=after 2.5 hours)	change from baseline to 2.5 hours
IL-6 change	IL-6 (change from baseline = after overnight fasting to post exercise (=after 2.5 hours)	change from baseline to 2.5 hours
hs-CRP change	hs-CRP (change from baseline = after overnight fasting to post exercise (=after 2.5 hours)	change from baseline to 2.5 hours

Secondary Outcome Measures

Outcome Measure	Measure Description	Time Frame
estradiol	17-beta-estradiol	baseline

Collaborators and Investigators

• Sponsor: University of Vienna
• Investigators: Principal Investigator: Markus Gassner, MSc, University Assistant; Study Director: Daniel König, Univ Prof, Professor of sports nutrition

Study record dates

Study Major Dates

• Study Start (Actual): May 17, 2022
• Primary Completion (Estimated): December 31, 2024
• Study Completion (Estimated): December 31, 2024

Study Registration Dates

• First Submitted: January 26, 2022
• First Submitted That Met QC Criteria: February 7, 2022
• First Posted (Actual): February 16, 2022

Study Record Updates

• Last Update Posted (Actual): December 5, 2023
• Last Update Submitted That Met QC Criteria: November 28, 2023
• Last Verified: November 1, 2023

More Information

Terms related to this study

• Additional Relevant MeSH Terms: Pathologic Processes; Inflammation
• Other Study ID Numbers: 2021-00743

Plan for Individual participant data (IPD)

• Plan to Share Individual Participant Data (IPD)?: NO

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: No
• Studies a U.S. FDA-regulated device product: No