Skeletal Muscle and Adipose Tissue Responses After Acute Exercise: Comparing Effects of Three Different Intensities (3X)

The study will assess the exercise-induced changes in chromatin accessibility and gene expression in a cell type-specific manner in skeletal muscle from healthy adults in response to exercise at three different intensities - 1) low-intensity continuous exercise, 2) moderate-intensity continuous exercise, and 3) high-intensity interval exercise.

Study Overview

• Status: Completed
• Conditions: Cellular Responses Within Skeletal Muscle in Response to Exercise of Different Intensities; Relationship Between the Cellular Responses in Skeletal Muscle and the Changes in the Metabolomic Profile; Changes Within Adipose Tissue in Response to Exercise at the Three Different Intensities
• Intervention / Treatment: Behavioral: Low intensity exercise session; Behavioral: Moderate intensity exercise session; Behavioral: High intensity exercise session

• Study Type: Interventional
• Enrollment (Actual): 45
• Phase: Not Applicable

Contacts and Locations

Study Locations

• United States
  • Michigan
    • Ann Arbor, Michigan, United States, 48109
      • University of Michigan

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 18 years to 40 years (Adult)
• Accepts Healthy Volunteers: Yes

Description

Inclusion Criteria:

• Age:18-40
• Body Mass Index: 20-30 kg/m2
• Must engage in regularly planned aerobic-type exercise at moderate-vigorous intensity, 3 times per week for at least 30-60 minutes/session
• Women must have regularly occurring menses and must be premenopausal
• Subject willingness to opt-in for the Michigan Genomics Initiative (MGI) Repository

Exclusion Criteria:

• Evidence/history of cardiovascular or metabolic disease.
• Taking anti-coagulate medications or medications known to affect metabolism or inflammation
• Weight instability ≥ ±3kg in the last 6 months
• Tobacco or e-cigarette users
• Women must not be pregnant or actively lactating

Study Plan

How is the study designed?

Design Details

• Primary Purpose: Basic Science
• Allocation: Randomized
• Interventional Model: Parallel Assignment
• Masking: None (Open Label)

Number of Arms

3

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
Experimental: low-intensity continuous exercise; subjects will exercise at 30% maximal oxygen consumption (VO2max) for 45-60 minutes	Behavioral: Low intensity exercise session; subjects will exercise at 30% maximal oxygen consumption (VO2max) for 45-60 minutes
Experimental: moderate-intensity continuous exercise; subjects will exercise at 65% maximal oxygen consumption (VO2max) for 30-45 minutes	Behavioral: Moderate intensity exercise session; subjects will exercise at 65% maximal oxygen consumption (VO2max) for 30-45 minutes
Experimental: high-intensity interval exercise; subjects will completed 10x1minute exercise intervals at maximal oxygen consumption (VO2max) with 1 minute active recovery between	Behavioral: High intensity exercise session; subjects will completed 10x1minute exercise intervals at maximal oxygen consumption (VO2max) with 1 minute active recovery between

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
post-translational modifications of skeletal muscle oxidative enzymes	Western blot	30 minutes before exercise
post-translational modifications of skeletal muscle oxidative enzymes	Western blot	immediately after exercise
post-translational modifications of skeletal muscle oxidative enzymes	Western blot	60 minutes after exercise
post-translational modifications of Adipose tissue metabolic proteins	Western blot	15 minutes before exercise
post-translational modifications of Adipose tissue metabolic proteins	Western blot	75 minutes after exercise
mRNA expression of skeletal muscle oxidative enzymes	qPCR	30 minutes before exercise
mRNA expression of skeletal muscle oxidative enzymes	qPCR	immediately after exercise
mRNA expression of skeletal muscle oxidative enzymes	qPCR	60 minutes after exercise
mRNA expression of adipose tissue metabolic enzymes	qPCR	15 minutes before exercise
mRNA expression of adipose tissue metabolic enzymes	qPCR	75 minutes after exercise

Collaborators and Investigators

• Sponsor: University of Michigan

Study record dates

Study Major Dates

• Study Start (Actual): December 13, 2021
• Primary Completion (Actual): June 23, 2023
• Study Completion (Actual): June 23, 2023

Study Registration Dates

• First Submitted: April 8, 2022
• First Submitted That Met QC Criteria: May 3, 2022
• First Posted (Actual): May 9, 2022

Study Record Updates

• Last Update Posted (Actual): July 26, 2024
• Last Update Submitted That Met QC Criteria: July 25, 2024
• Last Verified: July 1, 2024

More Information

Terms related to this study

• Other Study ID Numbers: HUM00204857

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: No
• Studies a U.S. FDA-regulated device product: No